We narrowed to 637 results for: erf

1. Adeno-associated Viruses (AAVs) for Genome Editing

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   ...cell types, now you know that AAVs can provide a powerful boost to genome editing experiments. We’re excited...

2. An Integrin Antibody Toolkit from IPI

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   ...integrins at non-ligand-binding sites and do not interfere with integrin function. Overall, the synthetic...

3. Transferable Skills Guide: Conflict Resolution

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   ...form of (hopefully constructive) conflict. As you perform the experiments to address a reviewer’s concerns...

4. Tips for Getting a Faculty Position

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   ...weeds. Check out Joanne Kamens's "10 Steps to a Perfect Science Talk." 4. The chalk talk, simple in principle...

5. CRISPR 101: Homology Directed Repair

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   ...targeted-break technology coupled with HDR is a powerful site-specific knock-in tool that can be harnessed...

6. New Optogenetic Tools for Cytoskeleton and Membrane Control

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   ...recruiting proteins to the cell membrane isn’t powerful enough, what about altering the chemical composition...

7. Antibodies 101: Single Chain Fragment Variables (scFvs)

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   ...functional scFvs; however, some individual scFvs performing better in one configuration than the other (Sandomenico...

8. Plasmids 101: Biotinylation

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   ...relatively small, water soluble molecule that does not interfere with the macromolecules it is added to. Biotin...

9. Transferable Skills Guide: Time Management

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   ...quick and useful tasks will swiftly give you the wonderful feeling of accomplishment while the longer tasks...

10. Using Phosphoserine to Study Protein Phosphorylation

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    ...generate non-phosphorylated recombinant protein and perform in vitro kinase reactions. Often, however, researchers...

11. Evolution of Lab Techniques

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    ...upon the incorporation of a ddNTP. Researchers perform four different reactions, one for each radiolabelled...

12. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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    ... restriction site for linearization. This is a perfect case for the use of Gibson assembly combined with...

13. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

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    ...of the  Brainbow multicolor labeling system - a perfect example of the continued utility of Cre-lox. Check...

14. Plasmids 101: Common Lab E. coli Strains

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    ...gene of interest, painstakingly cloned into the perfect empty backbone, made sure to add the right tags...

15. Is this the right place for me? 8 tactics for choosing a lab

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    ... Or is it teamwork that brings out your best performance and learning? Everyone’s different so give some...

16. Viral Vectors 101: AAV Variables That Matter

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    ...first amount that looks effective. Ultimately, the perfect “high titer” is one that optimizes gene expression...

17. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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    ...Michael, S., Kuleshova, E., Ojala, D., Huang, C.-C., Gerfen, C. R., Schiller, J., Dudman, J. T., Hantman, A...

18. Quick Guide to All Things Lentivirus

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    ...important to be aware that lentiviral particles are a powerful way to transduce cells - even your own! You should...

19. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

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    ...Orientation) or DO (Double-floxed Orientation)) is a powerful tool that provides precise temporal and spatial...

20. When Fidelity Matters: A frank discussion about ligase fidelity

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    ...polynucleotide ligase and RNA capping enzyme superfamily of covalent nucleotidyltransferases." Current...