We narrowed to 639 results for: erf

1. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

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   ...tagged protein because of its size. This type of interference is known as steric hindrance. For instance, ...

2. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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   ... intestinal crypt, and since then has become a powerful tool for understanding cell proliferation. Inducible...

3. Plasmids 101: Biotinylation

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   ...relatively small, water soluble molecule that does not interfere with the macromolecules it is added to. Biotin...

4. New Optogenetic Tools for Cytoskeleton and Membrane Control

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   ...recruiting proteins to the cell membrane isn’t powerful enough, what about altering the chemical composition...

5. An Integrin Antibody Toolkit from IPI

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   ...integrins at non-ligand-binding sites and do not interfere with integrin function. Overall, the synthetic...

6. Replacing Paper: Tips for Choosing an Electronic lab Notebook

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   ...notes etc. Detailed records about every action performed in the lab (user, date, time, action) are required...

7. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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   ...ease of use, 2) time savings, and 3) ability to perform single site or multi-site saturation mutagenesis...

8. Using Phosphoserine to Study Protein Phosphorylation

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   ...generate non-phosphorylated recombinant protein and perform in vitro kinase reactions. Often, however, researchers...

9. Evolution of Lab Techniques

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   ...upon the incorporation of a ddNTP. Researchers perform four different reactions, one for each radiolabelled...

10. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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    ... restriction site for linearization. This is a perfect case for the use of Gibson assembly combined with...

11. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

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    ...of the  Brainbow multicolor labeling system - a perfect example of the continued utility of Cre-lox. Check...

12. Plasmids 101: Common Lab E. coli Strains

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    ...gene of interest, painstakingly cloned into the perfect empty backbone, made sure to add the right tags...

13. Viral Vectors 101: AAV Variables That Matter

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    ...first amount that looks effective. Ultimately, the perfect “high titer” is one that optimizes gene expression...

14. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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    ...Michael, S., Kuleshova, E., Ojala, D., Huang, C.-C., Gerfen, C. R., Schiller, J., Dudman, J. T., Hantman, A...

15. Is this the right place for me? 8 tactics for choosing a lab

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    ... Or is it teamwork that brings out your best performance and learning? Everyone’s different so give some...

16. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

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    ...Orientation) or DO (Double-floxed Orientation)) is a powerful tool that provides precise temporal and spatial...

17. A History of Genome Engineering in Popular Culture

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    ...replicants which are genetically engineered by a powerful corporation to serve as slaves.  Meganucleases...

18. When Fidelity Matters: A frank discussion about ligase fidelity

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    ...polynucleotide ligase and RNA capping enzyme superfamily of covalent nucleotidyltransferases." Current...

19. Quick Guide to All Things Lentivirus

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    ...important to be aware that lentiviral particles are a powerful way to transduce cells - even your own! You should...

20. Typing CRISPR Systems

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    ...become a natural successor to traditional RNA interference, as it can target and degrade select RNA transcripts...