We narrowed to 903 results for: nes
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TypeBlog Post...2006, we saw an increase in popularity of empty backbones for shRNA expression, such as pLKO.1 puro and ...shRNA for use as a negative control, and empty backbones such as pBABE-puro for retroviral expression and...
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What's New in CRISPR - December 2019
TypeBlog Post...Yachie’s lab recently developed a base editor that combines the abilities of C-to-T and A-to-G base editors...TadA) to a catalytically impaired Streptococcus pyogenes Cas9. They called this editor Target-ACE (adenine... -
Seeing Red: Simple GFP Photoconversion
TypeBlog Post... dynamics can be undertaken using available GFP lines. Like many great scientific discoveries, this one...for microscopy enthusiasts! Addgene has empty backbones containing GFP variants used by Sattarzadeh et... -
Three Tips to Organize Your Lab Notebooks in the New Year
TypeBlog Post...you experiments, keep track of upcoming grant deadlines, and sync everything to your calendar. Ditch the...Tasks to create lists of your upcoming tasks, set deadlines, and arrange automatic email reminders. These ... -
Scientific Sharing in the Time of COVID-19: Databases and Resources
TypeBlog Post...involved with COVID-19 to researchers in other disciplines (bioinformatics, image analysis, AI…). How to...new Center for COVID-19 Response and Pandemic Preparedness (CCRP2) at Rutgers University not only supports... -
2023 and 2024: Reflections and Looking Ahead
TypeBlog Post...future. Over the years, we have benefited from the business rigor and efficiencies typically adopted by for-profit... Chonnettia Jones... -
Cre-ating New Methods for Site-specific Recombination in Drosophila
TypeBlog Post...and KD were shown to be functional in cultured Chinese hamster ovary (CHO) cells, as well as in cortical...activity regulation. Heidmann D, Lehner CF. Dev Genes Evol. 2001 Sep;211(8-9):458-65. doi:10.1007/s004270100167... -
Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner
TypeBlog Post...Public library. Being in the presence of scientific heroines is always inspiring. I was especially excited ...been able to use CRISPR/Cas9 to generate mice with genes knocked out in the insulin signaling cascades in... -
Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation
TypeBlog Post...site. In the SPARC system, PhiC31 irreversibly recombines one of the two competing attP sites to drive ...read-through transcripts of the effector gene. Effector genes, any gene that you want to control the expression... -
Plasmids 101: What is a plasmid?
TypeBlog Post...gene. Vital component for expression vectors: determines which cell types the gene is expressed in and... flexibility, versatility, safety, and cost-effectiveness enable molecular biologists to broadly utilize... -
Running for Rare Disease, Running for FOP, Running for AJ
TypeBlog Post... Rare Disorders (NORD). NORD raises money and awareness for rare disease and helps patients find the correct...an organization dedicated to raising funds and awareness of FOP. Please consider running with us or making... -
Addgene at the ASM Microbe 2016 Conference
TypeBlog Post...raffled off one of our highly sought-after "nice genes…" tshirts, and the lucky winner was Shagun Sharma...to be had at ASM Microbe as well as the serious business of science. Two symposia on Sunday afternoon were... -
Management for Scientists: Managing vs. Leading
TypeBlog Post...any goal or finishing any project. Doers are the ones who do the work Doers focus on understanding what...Marshall Goldsmith, with Mark Reiter Harvard Business Review 10 Must Reads: On Leadership (Articles ... -
Choosing a Good Mentor for Scientists
TypeBlog Post...postdocs do to ensure the advisors they choose will be ones that help them succeed after the training period... familiarity and confidentiality to ensure more honest answers. Ask people who work in the labs next door... -
Single-cell tracking of lineage and identity with CellTag
TypeBlog Post...partially reprogrammed and re-expressed fibroblast genes. Most cells ended up taking the ‘dead-end’ path....CellTag is a combinatory cell-indexing tool that combines scRNA-seq and lineage tracking to track the dynamics... -
Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi
TypeBlog Post...was then screened through successive rounds of mutagenesis for the brightest teal fluorescent proteins that...scientists from Florida State University, they used a mutagenesis approach and found that specific amino acid changes... -
New and Upcoming Viral Vectors - Spring 2019
TypeBlog Post...with different properties (for example, overall brightness or on/off kinetics) that makes them suitable ...pAAV.hSyn-FLEX.iGABASnFR) has good membrane localization and brightness. The F102G variant (pAAV.hSyn-FLEX.iGABASnFR.... -
CRISPR Challenges: Standardization and Homology Directed Repair
TypeBlog Post...Editing Consortium hopes to evaluate genome editing pipelines and generate a common lexicon for genome editing...non-homologous end joining (Alt-NHEJ), has been harnessed for CRISPR knock-in applications. CRISPR 101: ... -
Addgene Begins Distribution of Recombinant Antibodies
TypeBlog Post...derived were tested for specificity in knock-out lines or animals. Addgene will continue to add more antibodies...antibodies, for neuroscience research and other disciplines, over time. The resource may also expand to include... -
RUBY-Red Siliques
TypeBlog Post...fluorescent proteins. Selection using resistance genes usually involves killing the untransformed seedlings...then green offspring can be selected instead of red ones. Other innovative uses of RUBY are possible, for...