Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 1,045 results for: ins;

Showing: 311 - 320 of 1045 results

  1. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    ...the proteins they label. In fact, a recent study of 400 proteins found that 20% of tagged proteins did... protein must be tested carefully to ensure it retains the attributes of the native protein. Since this...notably high solubility, limited affinity for other proteins, and accessibility to an antibody. From their ...candidates, they selected 12 candidate protein domains from non-vertebrate systems. Preliminary testing...sophisticated interactions with multiple other proteins. Again, little to no change in cell morphology...Series. Get the Inntag Plasmids Tag endogenous proteins with CRISPR Fluorescently Tag Your Protein of ...

  2. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    ... protect you and your institution, and almost all universities and institutions require them for material...protective of both the providing institution and the receiving institution. Therefore, many technology transfer...transfers. The UBMTA was drafted by the National Institutes of Health (NIH) and adopted by the Association...benefits for both the providing and receiving institutions and their respective researchers. Check out ...knowing your rights – and the rights of your institution – are protected while we handle all the paperwork... to you, or we can send it directly to your institution’s MTA coordinator. Organizations that have opted...

  3. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ...DNA-binding molecule, such as Zinc-finger or TAL proteins, can be used. The Fujii lab demonstrated this ...spectroscopy studies allowed for the identification of proteins associated with the IRF-1 locus. Figure...was an intensive process. One method required insertion of a recognition sequence into the genomic DNA...that do not use dCas9, require Zinc-finger or TAL proteins targeting a specific genomic locus, which can ... addition to the identification of DNA binding proteins, this method can characterize nucleic acid-nucleic... D4Z4 array is modulated by DUX4-induced MBD3L proteins." Elife 7 (2018): e31023. PubMed PMID: 29533181...genomic regions and identification of associated proteins by engineered DNA-binding molecule-mediated chromatin...

  4. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type
    Blog Post
    ...constructing fusion proteins, check out our article: Fluorescent Proteins 101: GFP Fusion Proteins - Making the...factors. For example, FRET requires that fusion proteins be in close proximity and present in large enough...protein-protein interactions that uses split fluorescent proteins tethered to potential protein interaction partners...difficult with FRET, as the overexpression of fusion proteins it requires can lead to aberrant subcellular localization...localization of BiFC constructs should also be compared against those of their endogenous counterparts (by immunocytochemistry...call for a simple, yes-no answer — i.e., do two proteins interact or not? — FRET is preferable when the...Visualization of Interactions among bZIP and Rel Family Proteins in Living Cells Using Bimolecular Fluorescence...

  5. Typing CRISPR Systems

    Type
    Blog Post
    ...and understanding of CRISPR, and perhaps provide insights and ideas for your own research! Classification...bacteria and nearly 100% in archaea, though Class 1 remains largely underutilized compared to Class 2. Class...effector complex but are defined by the accessory Cas proteins in the Cascade complex. Despite a tenuous grasp... genes, Cas1 and Cas2, which are critical for inserting short fragments of foreign DNA into the CRISPR...CRISPR in more depth, the Jennifer Doudna lab maintains an encyclopedia of Class 2 CRISPR systems called...a CRISPR-associated transposase (CAST) that can insert large fragments of DNA. The V-F variants, which...discovery algorithms find new candidates for CRISPR proteins. A type VII is already on the horizon (Altae-Tran...

  6. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ...recombination is the process by which nearly all domains of life repair genomic damage, specifically double...sequence homologous to the chromosomal site of insertion. The linear PCR product is introduced into the...the cell by direct transformation. A given insert will typically contain both a protein modification sequence...imaging studies rely on direct fusion of fluorescent proteins (FPs) to a yeast gene of interest. These fluorescently...abundance, movement, and localization of individual proteins, but also investigate protein-protein interactions...interest, allowing for easy capture and detection of proteins and complexes, without the artifacts sometimes... PCR product from this collection will yield an insert with the format (protease site)-6xGly linker-(epitope...

  7. Hot Plasmids - August 2020

    Type
    Blog Post
    ...cleave affinity tags from purified recombinant proteins at small and large scale.  This new technique ...technique involves selection in the cytosol (instead of on the yeast extracellular surface) which enables tracking...demonstrated to work for evolution of other target proteins and might be useful for your studies.  Find the... Angela Abitua Genetically encoded fluorescent proteins are often used to label  specific structures, ...within cells. However, sometimes these fluorescent proteins might not localize to the right places, which ...to improve nuclear localization of fluorescent proteins, they found that adding a triple nuclear localization...in the cytosol of E. coli, however the nanobody proteins tend to get stuck in inclusion bodies. Takayuki...

  8. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    ...co-repressor important in development. Groucho family proteins are found in flies, worms, frogs, mice, and even...the Shivdasani Lab from the Dana Farber Cancer Institute! Sonic Hedgehog, an important regulator of embryonic...named by a postdoc in Cliff Tabin’s lab who took inspiration from a comic book his daughter was reading. SHH...synthetic transposon system that can be used to insert specific pieces of DNA into vertebrate chromosomes... et al., 1991, Jordan and Karess, 1997), which contains the regulatory regions that are necessary for ...spaghetti? I actually couldn’t find the specific origins of the name but some of our Addgenies had an idea...plasmid in our repository, plasmid 39624, that contains cmr.   tinman and bagpipe, two genes important...

  9. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...you produce viral vectors using viral envelope proteins from another virus to either restrict or broaden... but you use a different envelope glycoprotein instead of the wild-type glycoprotein. The pseudotyped ...protein is encoded on the envelope plasmid which contains a promoter, the envelope gene, and a polyA tail...virus:  Lowering cytotoxicity. Different envelope proteins have different levels of cytotoxicity and the ...pseudotyping methods with other viruses in a BSL-2 lab instead of a BSL-3 or BSL-4 lab. For more details, check...in serum (DePolo et al., 2000). Other envelope proteins for pseudotyping While VSV-G is the most commonly...for pseudotyping, there are many more envelope proteins that can be used for specific purposes as described...

  10. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...split protein like Cas9 is to use split inteins. Split inteins are a pair of naturally occuring polypeptides...toxicity. More AAV-based CRISPR systems Smaller Cas proteins SaCas9 isn't the only CRISPR enzyme that’s small...advantage that it can be turned off by anti-CRISPR proteins. The Sontheimer lab used NmeCas9 with AAV to edit...disease-causing loci in mouse liver.  Two other smaller Cas proteins include Cas12b and CasX. While there are no papers...papers yet published that deliver these Cas proteins with AAV, at just 1,108 and 986 amino acids, respectively...polypeptides that, when at the ends of two proteins, mediates protein trans-splicing, similar to an intron...developed a split intein spCas9-AAV toolbox that retains the gene-targeting capabilities of full-length ...
Showing: 311 - 320 of 1045 results