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  1. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    ... their materials. The new office in London is dedicated to helping them find their way to new scientific... then she has worked in the the UK, California, Africa, and Australia, viewing science as a global collaboration...

  2. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ...CRISPR array. The CRISPR array is composed of identical repeat sequences and variable spacer sequences...simplifying the CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer to the programmable...

  3. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    ...Their work involves coordination and can require technical mastery. Scientists need to be doers for a long...in the way of the doers and by ensuring good communication. A manager must both hire and fire to make sure...

  4. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...with the newest CRISPR technologies and their applications can be exhausting. A quick search for "CRISPR...puromycin selection. You could then confirm this purification by performing a genotyping assay (such as Sanger...

  5. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ...contains the proteins needed for the virus to replicate,  and the transfer plasmid which contains the ... sites in the ITRs. Regular Sanger sequencing typically fails to sequence through ITRs due to their secondary...

  7. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ...of autophagic substrates inside the lysosome. Historically, methods for studying autophagy focused on counting... autophagic activity, and are a more reliable indicator of autophagic activity. The aim of this post is...

  10. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ...positions posted on recruitment websites, this job specifically looked for a researcher with 3 years+ of cancer...on the marketing or business background of the applicant. However, although this position did look for ...

  11. How to Lead a Great Meeting

    Type
    Blog Post
    ... dialogue and community among participants, clarification - new and useful information, and invigoration...be the representative in the meeting and then communicate outcomes to the rest of their team. Here's a ...

  13. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...pipetting experience. However, after a few months of dedicated time at BosLab, he became our bacterial transformation... for discovering novel therapeutics. Dave is a chemical biologist with a Ph.D. from UNC-Chapel Hill and...

  14. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ...FiercePharma), or sign up to be alerted about their new publications via Pubmed, so you can congratulate them when...really understood what I was going through. (Parenthetically, when I called the lab to ask my benchmate ...

  15. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    ...Christopher Harvey’s lab. According to their publication (Chettih & Harvey, 2019), “this localization ...Recombinase AAV Targeting and manipulating specific, genetically-defined neuronal populations with intersectional...

  16. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ... the following, which are later printed for my physical lab notebook, along with any figures generated.... She is particularly interested in science communication and epigenetics. Follow her on twitter @kc_palozola...

  17. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...end joining (NHEJ). In these reporters, editing typically results in a change in the fluorescent readout...stimulated homology-directed repair. Journal of Biological Chemistry 292:6148–6162 . https://doi.org/10.1074...

  19. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...plasmid. You should also verify the strain and the efficacy of your competent cells (when they get old, they...constructs. His work was acknowledged in hundreds of publications including Cell, Nature, and other journals. ...

  20. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...downstream of the gene to be transcribed, and typically occur directly after any 3’ regulatory elements...rho-dependent and rho-independent. Rho factor is a helicase which assists RNA polymerase in the termination...

  21. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ... tool to identify a protein of interest in a complicated solution like cell lysate. But they can be a ...reacts with antibodies, so thorough washing is critical. Assess the stripping Now it’s time to check ...

  23. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...Also, it remains challenging to screen for the genetically modified strains that contain your newly tagged...Toolkit for High-Throughput CRISPR/Cas9 Gene Modification in Caenorhabditis elegans.” Genetics. 202(4)...

  24. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...Preger et al., 2020. Preger et al., Journal of Biological Chemistry 2020. https://doi.org/10.1074/jbc.RA120.01289...expression selectively in Parvalbumin containing cortical interneurons. We now provide ready-to-use AAV ...

  25. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ... to deaminate cytidine and used dRanCas13b (catalytically inactive Cas13) to target the cytosine deaminase...Addressing the potential use of RESCUE in therapeutic applications, the authors tested various dRanCas13b truncations...

  26. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...created by making some or all of the following modifications: 1) increased concentration of Taq polymerase...stretches of sequence could be used. However, a practical limitation is sequencing depth and cost. You need...

  27. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ... the open conformation (Cofsky et al., 2022), indicating that there is more to learn about this process...conformational state of the HNH domain also allosterically controls RuvC activity through a hinge region...
Showing: 931 - 960 of 1075 results