We narrowed to 710 results for: BASE;
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TypeBlog Post... in the envelope and are targets for complement-based killing by anti-alphaGal antibodies. The VSV-G envelope...
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Using Video to Share Your Science: We Share Science
TypeBlog Post...discipline are welcome. Up to this point most videos are based on research that has been published, but many discuss... -
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog Post...representing ~85% of the postdoctoral workforce. Based on our communications with and research on these... -
Tips for CRISPR Gene Editing in Mice
TypeBlog Post...should first develop primers to the approximately 500 base pair sequence surrounding your gRNA. Once amplified... -
Important Considerations in Optogenetics Behavioral Experiments
TypeBlog Post...could reveal something fascinating. Behavior is based primarily on careful observation of what an animal... -
AAV ddPCR Titration
TypeProtocol...The dilution series outlined in this protocol are based on an AAV titer range of 5E+12–5E+13 GC/mL, where... -
Plasmids 101: Expressing shRNAs for RNA Interference
TypeBlog Post... smaller, with the full sequence being around 60 base pairs. This is much shorter than miRNA plasmids,... -
March for Science
TypeBlog Post... the meaning of the word ‘fact’. Policy must be based on our most accurate interpretation of the world... -
Using Phosphoserine to Study Protein Phosphorylation
TypeBlog Post...fail to recapitulate the true steric and charge-based nature of the phosphoryl-modification; they are ...appreciated. PhosphoSitePlus, one of the most inclusive databases of post-translational modifications, identifies... -
CRISPR Library Amplification
TypeProtocol... but care must be taken to perform adequate NGS based QC to ensure no change in representation compared... -
Hot Plasmids: Summer 2024
TypeBlog Post...Genome-wide programmable transcriptional memory by CRISPR-based epigenome editing. Cell, 184(9), 2503–2519.e17. ... -
Finding Your Science Policy Path
TypeBlog Post...Think a university system who develops evidence-based university policy to combat sexual harassment in... -
Antibodies 101: Validation
TypeBlog Post...same conditions. Example: You want to sort cells based on the expression of a new receptor that is of interest...attempts by other means, such as through antibody databases like Antibodypedia or through your antibody provider... -
Treating Muscular Dystrophy with CRISPR Gene Editing
TypeBlog Post...WH, Reddy TE, Gersbach CA. Multiplex CRISPR/Cas9-based genome editing for correction of dystrophin mutations... -
Lentivirus ddPCR Titration
TypeProtocol...The dilution series outlined in this protocol is based on an LV titer range of 1E+05–1E+09 TU/mL, where... -
A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression
TypeBlog Post...interest. Determine injection titers and volumes based on the serotype Since each serotype also exhibits... -
Viral Vectors 101: Optogenetic Tools
TypeBlog Post...electrical activity to gene expression, and even CRISPR based gene editing. But, how do you decide which tools... -
Plasmids 101: Dimers and Multimers
TypeBlog Post...deficiency independently increase the frequency of base substitution in human cells. J Biol Chem. 2011 Mar... -
Finding Your Perfect Job After University
TypeBlog Post...Addgene’s main offices are in Cambridge MA, while I’m based in London) does bring its own challenges. I’ve had... -
Which Fluorescence Microscopy Technique is Best for Me?
TypeBlog Post.... Eggeling C, Willig KI, Sahl SJ, Hell SW. Lens-based fluorescence nanoscopy. Quarterly reviews of biophysics...