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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 641 results for: CAR

Showing: 621 - 640 of 641 results

  1. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ... process is shown graphically in the following cartoon, in which we are adding EcoRI and NotI sites to...Verify your Plasmid by Sequencing PCR based cloning carries a much higher risk for mutation than restriction...

  2. Western Blot

    Type
    Protocol
    ...microcentrifuge tube. Centrifuge for 5 min at 100 x g . Carefully remove supernatant. Resuspend cell pellet in appropriate...transfer stack. Set the Top Stack to one side and discard the white separator. Keep the Bottom Stack in the...

  3. Ligation Independent Cloning

    Type
    Protocol
    ...restriction sites from the final product (no "cloning scars"). A “stuffer” sequence allows for electrophoretic...

  4. Centrifugation

    Type
    Protocol
    ... When you are finished spinning your samples, carefully remove them from the centrifuge so as to not dislodge...

  6. Gibson Assembly Protocol

    Type
    Protocol
    ...almost any two fragments regardless of sequence. No scar between joined fragments. Fewer steps. One tube ...

  7. Colony Formation Titering Assay

    Type
    Protocol
    ...the stain. Wash cells 3x with 1 mL of PBS, being careful not to disturb the colonies. Count the colonies...

  10. Chemogenetics Guide

    Type
    Guide
    ...are currently three Gq DREADDs based on human muscarinic receptors: hM1Dq, hM3Dq, and hM5Dq, however, ...There are currently two Gi DREADDs based on human muscarinic receptors, hM2Di and hM4Di, and a Gi DREADD based...erythrocyte β-adrenergic receptor with a rat M3 muscarinic receptor. This DREADD was shown to have a small...DREADD, termed Rq(R165L), is based on the human M3 muscarinic receptor and has not been used in vivo . Figure...ligands, and signaling properties. DREADD Ligands Muscarinic-receptor based DREADDs were engineered to respond... all been explored as alternative ligands for muscarinic-receptor based DREADDs. Compound 21 has similar...FDA and EMA approved. One pitfall of a suite of muscarinic-based DREADDs that all respond to the same ligands...

  11. Adenovirus Guide

    Type
    Guide
    ...adenoviral (rAdV) vectors in order to deliver genetic cargo into cells. Most rAdV vectors are based on adenovirus...transgenes of interest. The size of the genetic cargo that can be included between the ITRs is limited... which not only frees up more space for genetic cargo but also improves safety. In order to produce rAdV...allowed for the inclusion of ~6.5 kb of genetic cargo in first-generation rAdV vectors. E3 is involved... possibility of accommodating ~36 kb of genetic cargo. Production of HCAdV vectors requires using an additional...of the HCAdV vector production is to ensure no carryover from the HV in final viral preparations. Several...period of time or when needing to package larger cargo. CRISPR-Cas9 Delivery As compared to other viral...

  12. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...viral (rAAV) vectors in order to deliver genetic cargo into cells. Recombinant AAV constructs are designed... transgene of interest. The size of the genetic cargo that can be included between the ITRs is limited...for viral vector production, rAAV vectors do not carry the genes to express Rep78 and Rep 68 proteins and...packaging plasmid used during production, as it carries the Cap region responsible for the production of... packaging capacity Another strategy to deliver cargo too large to fit within the rAAV capsid, such as...sequence at its 3' end, while the second vector carries the 3' portion of the gene with a splice acceptor...genes makes use of split inteins, proteins that carry out a naturally occurring process known as protein...

  13. CRISPR Guide

    Type
    Guide
    ...CRISPR plasmid will have all desired genomic edits carried out by Cas9. Multiplexing applications include ... and delivered to specific genomic locations to carry out unique tasks. We’ll discuss more Cas9 fusion...location in the genome, where the transposase then carries out integration of donor DNA. Both types of CASTs...with the sequence, so the edit is not considered “scarless”. Figure 7: Overview of CRISPR transposases Browse...that displays improved specificity, and editors carrying an ADAR truncation that retain RNA editing capabilities... Yang, L., Esvelt, K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013)...Jaenisch, R. (2013). One-Step generation of mice carrying mutations in multiple genes by CRISPR/CAS-Mediated...

  14. Educational Resources

    Type
    Guide
    ...Addgene’s materials, and career advice. Playlists include: Protocols Careers How-to Blog Read about plasmids... in molecular biology, laboratory research, and career development. eBooks Download any of our eBooks ...Viral Vectors 101 Fluorescent Proteins 101 Science Career Guide Videos Watch our collection of video content...Proteins CRISPR Viral Vectors Plasmid Technology Career Readiness Molecular Biology Learn about plasmids...

  15. Molecular Biology Reference

    Type
    Guide
    ... of Molecular Genetics The concept of genes as carriers of phenotypic information was introduced in the...fragments of double-stranded DNA that typically carry genes and can replicate independently from chromosomal...allowing for selection against any bacteria not carrying the plasmid. Additionally, the restriction enzyme...Concentration Ampicillin 100 mg/mL 100 µg/mL Carbenicillin* 100 mg/mL 100 µg/mL Chloramphenicol 25 mg/mL...µg/mL Tetracycline 10 mg/mL 10 µg/mL *Note: Carbenicillin can be used in place of ampicillin. Preparing...

  16. Optogenetics Guide

    Type
    Guide
    ...other species VChR1 Channelrhodopsin from Volvox carteri 570 Chronos High-speed, light-sensitive channel...channel from Chlamydomonas noctigama . ChrimsonR carries K176R mutation. 590 PsChR2 High-efficiency blue-shifted...Birdsey-Benson A, Cho YK, Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, et al. 2014. Independent optical excitation...tool for fast neural control derived from Volvox carteri. Nat Neurosci. 11(6):631-3. PMID 18432196...

  17. Addgene's eBook Collection

    Type
    Guide
    ...Overlay Select Science Career Guide November 2018 | First Edition About Science Career Guide November 2018...About The guide includes information on the many career options in science, advice on mentoring and management...

  18. Cloning

    Type
    Guide
    ...Restriction enzyme cloning also leaves behind a short scar in the DNA sequence and can be time consuming compared...entire cloning step (digestion and ligation) can be carried out in one reaction with a single type IIS restriction... the final product, with no undesired sequence ("scar") retained. A disadvantage of type IIS assembly ...

  19. Modular Cloning Guide

    Type
    Guide
    ...Cloning System and Toolkit Yeast Expression Elena Caro Bernat Plasmids for generating transcriptional units...Kit Bacterial Expression, CRISPR Veronica Godoy-Carter Plasmids for protein expression and CRISPRi experiments...

  20. Plan Your Experiment

    Type
    Guide
    ...save your lab valuable time and resources when carrying out CRISPR experiments and can be used as positive... producing stable cell lines, since the genetic cargo (in this case, CRISPR components) integrates into...
Showing: 621 - 640 of 641 results