We narrowed to 654 results for: CHI;

1. New Optogenetic Tools for Cytoskeleton and Membrane Control

   Type

   Blog Post

   ... stimulate and study these physical processes. Joachim Goedhart and colleagues in the Gadella Lab devised...

2. Antibodies 101: Introduction to Gating in Flow Cytometry

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   Blog Post

   ...expression, and viability. Gates are the parameters the machine uses to differentiate between variations in those...

3. Multiple Plasmids at a Low Price: Kits from Addgene

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   Blog Post

   ...purify proteins from E. coli, Bacillus subtilis, or Pichia pastoris depending upon your specific protein folding...

4. Site Directed Mutagenesis by PCR

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   Blog Post

   ... introduce a frame-shift mutation. This can be achieved using a restriction enzyme that recognizes a unique...

5. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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   Blog Post

   ...hurdles. The first is the delivery of the CRISPR machinery to all of the target cells. This goal may prove...

6. Treating Muscular Dystrophy with CRISPR Gene Editing

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   Blog Post

   ...editing faces the challenges above and more. One chief challenge is upregulating HDR in mature tissues,...

7. Plasmids 101: Common Lab E. coli Strains

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   Blog Post

   ...process of replicating your new plasmid: the Escherichia coli bacteria. It’s hard to count the number ...

8. Technologies Enabled by NanoLuc® Luciferase

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   Blog Post

   ... molecules. FRET sensors face challenges of photobleaching, autofluorescence, and, in the case of exciting...

9. Plasmids 101: Dimers and Multimers

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   Blog Post

   ... harboring large inserts are often dimers in Escherichia coli K-12. Plasmid. 1989 Mar;21(2):138-41. doi...

10. How to Design Your gRNA for CRISPR Genome Editing

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    Blog Post

    ...should not be undertaken lightly! Indeed, truly achieving the gold standard requires not one but two rounds...

11. Antibodies 101: Validation

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    Blog Post

    ... it’s important to know what you are aiming to achieve. In general, these are three core goals of antibody...

12. Plasmids 101: Using Transposons in the Lab

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    Blog Post

    ...target site (Fig. 3B). The INTEGRATE system can achieve ~100% integration of DNA up to 10 kB in size in...

13. A Guide to Getting Started in Undergrad Research

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    Blog Post

    ...researchers, as well as graduate students (your teaching assistants), about their experiences. Research...

14. Transferable Skills Guide: Teamwork

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    Blog Post

    ...knows how the physical kits are made and shipped; Chiara, the tech transfer expert who understands the somewhat...

15. Quick Guide to All Things Lentivirus

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    Blog Post

    ... PubMed PMID: 8602510. 3. Yasutsugu Suzuki and Youichi Suzuki (2011). Gene Regulatable Lentiviral Vector...

16. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

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    Blog Post

    ...edits without any reagent-grade endangered Martian chicory root, but considering this is a needle in a haystack...

17. Live and Let Dye: Self-Labeling Protein Tags

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    Blog Post

    ...ticker tape” method for intracellular recordings, switching between different colored ligands at different...

18. Sequencing Options for CRISPR Genotyping

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    Blog Post

    ...Mark J., et al. "Evaluation of TCR gene editing achieved by TALENs, CRISPR/Cas9, and megaTAL nucleases....

19. Custom CRISPR Screens & the Green Listed Software

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    Blog Post

    ... any of the alternative names works better. To achieve this you could e.g. copy the list of “Aliases” ...

20. Special Delivery: Fluorophore Targeting for FRET Studies

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    Blog Post

    ... 269-272. PubMed PMID: 9657724. 6. Kapanidis, Achillefs N., Yon W. Ebright, and Richard H. Ebright. "Site-specific...