We narrowed to 991 results for: Des

...transposon system is one such system which has been designed to perform gene transfer in vertebrates. The system...whose use is only likely to grow in the coming decades.  References 1. Kowarz E, et al. Optimized Sleeping...

...the measure of work is not just time spent at the desk, but it is worth it. This requires a focus on deliverables...characteristics of scientists in the work place as described by Sebastiano Massaro in his Nature Biotechnology...

...good and bad data, and knowing how to follow the design, execute, analyze, repeat pathway that is required...critically before diving in. Research projects are designed to train graduate students and postdocs to think...

...when the cells are grown on selective media, and provides those bacteria with a pressure to keep your plasmid...gene and acts as a marker for the mutation. When designing these types of experiments it is best practice...

...PDF at the end of this blog post. This document includes a list of questions to ask your potential advisors...some good pre-work you can find a lab that will deserve your hard work and an advisor that will be a lifelong...

...without end-point sequencing assays.  This tool was designed by putting together the coding sequence of a mCherry...MYC-CRISPR library targets E-boxes genome-wide. It was designed based on MYC-ChIP-seq data from several MYC-dependent...

...first NIH grant that Addgene has received. Addgene prides itself on being financially self-sustaining through..., Addgene’s interim Executive Director. “NABOR provides a new model for the creation and acquisition of...

...transport from the cell surface. Their toolkit includes a set of plasmids each containing an anti-GFP ...from A. victoria the functionalized nanobodies described here can be used to target any protein fused with...

...such as broccoli, cabbage and kale to name a few. Despite Arabidopsis’ utility to science, and its well-known...kicked off with the release of 20 education kits designed for use in K-12 and college-level instruction....

...one list can ever encompass them all. We can’t designate non-academic jobs as “alternative” anymore. ...Develop expertise areas quickly Jack/Jane of all trades More sources of training and resources within...

...the Addgene website. blog.addgene.org The blog provides tips for working with viruses including advice...on each viral prep’s specific material page and includes western blots as well as other expression data...

...of my review - "The manuscript by ZZZ et al., describes ... The authors share a wealth of data on ... ...Additionally, you can ask that data and resources described in the manuscript are accessible to the community...

...transduced using only protein. Read on for a description of the lab’s iTOP protein-only transduction method...transduction by osmocytosis and propanebetaine) to describe this method of protein transduction. Later studies...

...published in Nature Methods, Boyden and his team describe the discovery of a light-sensitive opsin from ...then, there has been a steady stream of papers describing new photosynthetic or photosensory molecules ...

...The natural CRISPR locus of a bacteria host encodes multiple guide RNAs (gRNAs) on a single array to ...applications, we provide detailed manual and online design examples of using Addgene plasmids hCtRNA_FT (#186715...

...visiting role is because the host lab or institute provides something that you can’t obtain at your current...skills and equipment! Preparing for your visit   Design and communicate a research plan Your time at the...

...biomass production techniques in the following decades, Phillips made the decision to release the K. phaffii...of K. phaffii lacks certain terminal mannose glycopeptides (which most other yeast possess) that are problematic...

...effects, both of happen with Cas13. CRISPR-Csm degrades both nuclear and cytoplasmic mRNA with 90-99% ...crystalizing the hfYFP structure, the group rationally designed GFP variants with similar capabilities. If you...

...to your community. For those of you who have the desire and ability to, some communities are looking for... results. Also consider using this framework to design your future experiments.  Use Addgene’s AAV Data...

...if you end up concentrating your AAV above your desired titer and want to dilute it, err on the side of...less buffer than you would to achieve the exact desired concentration to avoid over-diluting, and make ...