We narrowed to 756 results for: ELP
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TypeBlog Post...various promoters and other genetic manipulations to help restrict expression to a specific neuronal subtype...
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How to Deposit Your Plasmids with Addgene
TypeBlog Post...verified by next-generation sequencing. Please see the help center article, "Can I deposit a pooled DNA library... -
Prime Editing: Adding Precision and Flexibility to CRISPR Editing
TypeBlog Post...again? We’re glad you asked. Hopefully this table helps you keep them straight! Innovation Purpose... -
27 Hot Plasmids from 2016
TypeBlog Post...about depositing your new plasmids with Addgene to help expand the collection! The Jacks lab has made several... -
Adeno-associated virus (AAV) Plasmids
TypeCollection...without the use of a helper virus. Instead, specific gene products can be provided by helper plasmids (e.g.,...transfer plasmid). Helper Plasmid ID Plasmid Serotype Description PI 112867 pAdDeltaF6 All Helper plasmid for...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...Traditionally, AAV requires the presence of another "helper" virus, such as adenovirus or herpes virus, in ...replication. This requirement has been circumvented with “helper-virus free systems,” which enable the production...., pHelper) and specific packaging cell lines (e.g., HEK293 cells) during AAV production. Read our AAV...In order to generate infectious AAV particles, a helper plasmid (encoding adenovirus E4, E2A and VA) and... -
AAV Packaged on Request
TypeCollection...unshared. Help Center Check out Addgene’s Help Center, a searchable database of information to help you every...conditions of the applicable transfer plasmid. We will help you gain legal usage of the transfer plasmid from... more reproducible for scientists everywhere and help build a globally connected scientific community....distribution. Troubleshooting If your answer isn’t in the Help Center or you need more assistance with troubleshooting...troubleshooting, we want to hear from you! Email us at help@addgene.org .... -
Antibodies
TypeCollection...applications. View Guide Need Some Help? Browse some of our other resources to help you choose, store, or use ...distributes ready-to-use recombinant antibodies to help facilitate scientific research. We are building ... developed a variety of educational resources to help you choose and use antibodies, with an expert technical...Antibody Blog Posts Recipient Instructions Addgene Help Center Antibody Protocols Looking for Antibody Plasmids... -
All Antibodies
TypeCollection...our Suggest an Antibody form, or email help@addgene.org to help us improve our resources! Acknowledgments...Institutes of Health. Need Some Help? Browse some of our other resources to help you choose, store, or use ...Antibody Blog Posts Recipient Instructions Addgene Help Center Antibody Protocols... -
Antibody Production
TypeCollection...shown in your cart. Need Some Help? Browse some of our other resources to help you choose, store, or use ... on the antibody's catalog page or by contacting help@addgene.org . Some of the antibodies distributed...experiments were performed on your lot, please email help@addgene.org . Immunocytochemistry (ICC) Cells are...Antibody Blog Posts Recipient Instructions Addgene Help Center Antibody Protocols... -
DNA Service - Cloning Grade DNA
TypeCollection...plasmid available, but helps to inform us of which plasmids would be most helpful to requesting scientists...our Addgene Protocols for resources you may find helpful. Our Scientific and Customer Support Teams are ...are also available to provide assistance at help@addgene.org . Don’t See What You’re Looking For? At this... -
CRISPR Plasmids - Base Edit
TypeCollection...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource! Do you have ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!... -
mTOR Pathway
TypeCollection...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...The content for this page was generated with the help of David Sabatini . mTOR Pathway Color is used for...The content for this page was generated with the help of David Sabatini . Return to top mTOR Pathway -... -
CRISPR Plasmids - Prime Edit
TypeCollection...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...Engineering of the PE enzyme and related components have helped to improve the efficiency of editing, including...tools, check out our prime editing blog post . Need help designing a pegRNA? Try the DeepPrime (Link opens... -
Ras Pathway
TypeCollection...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...content and map for this page were generated with the help of Dominic Esposito and the Frederick National Laboratory... -
Adeno-associated virus (AAV) Guide
TypeGuide...between the transfer and the helper plasmid, and by providing the helper E1 gene in trans within the packaging...for the production and assembly of viral capsids. Helper plasmid — containing adenoviral genes not present...three production plasmids (transfer, packaging, and helper plasmid) are transfected into cells which then ...rAAV vectors. The E1 gene is not included in the helper plasmid and instead provided by the chosen cell...present as linear DNA molecules. In the absence of helper viruses, wild-type AAVs cannot enter the lytic .... The adenovirus E1A and E1B19K genes provide a helper function for transfection-based adeno-associated...outer capsid shell that protects the viral genome. Helper plasmid E4 in trans Adenoviral early gene that ... -
Neurodegeneration Research Collection
TypeCollection...assembled the following to help you find information and plasmid collections that may help with your neurodegeneration... rather than focusing on separate diseases, will help speed progress towards curing and treating them.... website and information resource dedicated to helping researchers accelerate discovery and advance development... -
Caltech Systemic Capsids
TypeCollection...preparation. Systemic Delivery AAV Capsid Choice Need help deciding which capsid to choose? The following decision...developed at the CLOVER Center at Caltech and can help guide your decision. Figure 1: Decision tree for...service, but lets us know what viruses would be helpful to the scientific community. Text Description for...Decision Tree The AAV decision tree graphic above helps you choose the best capsid for your cell type. First... -
Bacterial Expression Systems
TypeCollection...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...features. Addgene distributes many plasmids that can help researchers plan their experiments using bacterial... control over protein expression and activity, helping to conserve cellular resources otherwise spent ... -
Plasmids for Stem Cell Research
TypeCollection...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...different reprogramming methods with the goal of helping readers choose a strategy suited to their research...expression of key factors. The tables below will help you find plasmids available from Addgene that can... -
CRISPR Plasmids - Parasites
TypeCollection...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...The content for this page was generated with the help of Scott Lindner . Cut Fully functional CRISPR/Cas...