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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 629 results for: EMB

Showing: 621 - 629 of 629 results

  1. Molecular Biology Reference

    Type
    Guide
    ...reactions on different pieces of DNA to be able to assemble the whole puzzle. That’s where NGS (sometimes ...sequenced templates are then aligned to each other to assemble the entire sequence using bioinformatic methods...base pairs at a time. Nanopore uses specialized membranes containing nanopores (Figure 5). A motor protein... An electrical current runs through the porous membrane, and the changes in this current are recorded ...correlated with specific nucleotides and allow for the assembly of a complete sequence. Using this method, whole...

  2. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...and Cap regions necessary for the production and assembly of viral capsids. Helper plasmid — containing ... methods, including restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may have limited...infect cells and replicate autonomously. Human embryonic kidney 293T (HEK293T) cells are the most common...rAAV vector (∼5%). Gene fragments can also be reassembled via homologous recombination, when both sequences... infection risks occur with contact to mucous membranes or broken skin. Needle sticks and ripped gloves...

  3. Addgene's eBook Collection

    Type
    Guide
    ...Select Plasmids 101 September 2023 | Fourth Edition About Plasmids 101 September 2023 | Fourth ...Science Career Guide November 2018 | First Edition About Science Career Guide November 2018 | First...

  4. Adenovirus Guide

    Type
    Guide
    ...primarily encode for structural proteins needed to assemble icosahedral capsids and build new virions. Figure...by the packaging cell line, normally the human embryonic kidney 293 (HEK293) cell line. There is a rare... methods, including restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may have limited... infection risks occur with contact to mucous membranes or broken skin. The risk of exposure and reaction...

  5. Gamma-Retroviral Vector Guide

    Type
    Guide
    ... methods, including restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may have limited...Gamma-retroviral vectors can be packaged directly in human embryonic kidney 293T (HEK293T) cells (Figure 3A), similar... infection risks occur with contact to mucous membranes or broken skin. Needle sticks and ripped gloves...

  6. Lentiviral Vector Guide

    Type
    Guide
    ... methods, including restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may have limited...requires a production cell line, typically human embryonic kidney 293T (HEK293T) cells. Standard HEK293T ... infection risks occur with contact to mucous membranes or broken skin. Needle sticks and ripped gloves...

  7. Plan Your Experiment

    Type
    Guide
    ...read our three tips to improve HDR efficiency . Remember that HDR also requires donor DNA to serve as template...can transfect at high efficiency, such as human embryonic kidney 293 (HEK293) cells. For more difficult ...

  8. CRISPR Guide

    Type
    Guide
    ...against these foreign nucleic acids. Acr family members inhibit CRISPR by a variety of mechanisms, including...Mediated Genetic Screening in Mice with Haploid Embryonic Stem Cells Carrying a Guide RNA Library. Cell ...

  9. Chemogenetics Guide

    Type
    Guide
    ...cell surface receptors, GPCRs are seven-pass transmembrane proteins that bind a specific ligand. Ligand...
Showing: 621 - 629 of 629 results