... An overview of peptide synthesis. A ribosome interacts with mRNA and charged tRNAs to build a peptide...codon usage among different organisms. There are several codons that encode the same amino acid. Each organism... be harmful (Sauna and Kimchi-Sarfaty, 2011).  Overall, codon optimization may not always be the optimal...in vivo experiments and in biotechnology and therapeutics (Mauro and Chappell 2014).  Ultimately, it is...critical analysis of codon optimization in human therapeutics. Trends in Molecular Medicine 20:604–613  https...

.... Cas13a (previously referred to as C2c2), has several unique properties that further expand the CRISPR...other hand, uses only a short ~24 base crRNA that interacts with the Cas13a molecule through a uracil-rich...changes in the Cas13a molecule. Like Cas9, Cas13a tolerates single mismatches between the crRNA and target...interest (red) is amplified using Recombinase Polymerase Amplification (RPA) or Reverse-transcription ...knockdown to single gRNA expression. Although the collateral RNA cleavage response is well described in bacterial...

...plasmid-based experiments. What are control plasmids? Generally, control plasmids help to ensure that the observed...constitutively expresses a reporter protein (e.g., GFP or luciferase) that is either co-transfected with the test ...non-targeting control also assesses any effects of the general introduction of shRNA into your target cells. Positive...replicate controls: technical and biological. In general, technical replicates can be thought of as "plate...of Plasmid A in different aliquots of cells on several different days. Biological replicates control for...

...organized pipeline and accelerated the processing of our sequencing data.”  Recently, Seqera published a case...about our bioinformatics quest, which led us to Seqera Labs, a company that provides open source workflow...You can read it, and other case studies, on the Seqera website. ...

...communicator overall. Don’t Write in a Vacuum Your research papers should go through many iterations read ...Saturday Hack Day projects sought to make an interactive game that highlights just this point. As part... this, trainees need to be able to network and interact more with people who have taken a variety of career...write for a university publication, improving your general writing skills will help you in your academic publishing...

...coupling of Cre-lox with FLP-FRT recombination to generate additional recombination events, and the synthesis...and sea anemone mKate2.) They then successfully generated custom antibodies to each of these proteins and...analysis. To achieve even cell labeling, they generated farnesylated derivatives of the fluorescent proteins...previously visible with Brainbow-1 and -2. The general structure of Brainbow-1.0 is retained in Brainbow...constructs are stably maintained through at least six generations. In addition to the neuronal pThy1-Brainbow constructs...

... would thrive on this level of attention and interaction (and I did). Some of the information you will...advisor. They are always traveling, or too busy to interact regularly with the newer lab members. You must...careers?    Will you be encouraged to develop transferable skills by attending career events or being involved...your own choosing? Does the PI keep up on the literature and pass on relevant papers? Will you have access...resources on the Addgene blog Check out our transferable skills guide Learn about careers in science ...

...twice the length of the monomer (~11.6 kb). Image generated by SnapGene software.  Thanks to increasing...which has been used in plasmid vaccine and gene therapy development to examine plasmid topologies (e.g....stationary phase. Growing the culture at a lower temperature such as 30°C or optimizing the incubation time...Fishel RA, James AA, Kolodner R. recA-independent general genetic recombination of plasmids. Nature. 1981...294184a0. PMID: 7029308. Goebel W, Helinski DR. Generation of higher multiple circular DNA forms in bacteria...

...started by designing a novel topology, then iteratively generated 3D models and amino acid sequences that ...topology of protein” diagrams, which show the interactions between amino acids in an approximate, two-dimensional...crosslinked peptides proposed as a scaffold for a new generation of drugs, all developed with their protein design...

...ways of generating antibodies, for example through the use of less toxic adjuvants; 2) generating complex... The minimal fees from distribution funds our operation. Antibodies are glycoproteins that are part of...increased avidity to high profile antigens; and 4) generating mAbs against new cancer biomarkers. We believe...

...) (Miller et al., 2005). Although this tight interaction is not covalent, later work showed that introducing...ER and mitochondria reveals dynamic organelle interactions. a) Labeling scheme. b) COS-7 cell ER labeled...without any cloning at all! As mentioned above, several variants of these tags have been developed over...https://doi.org/10.1021/cb900062k Gautier, A., Juillerat, A., Heinis, C., Corrêa, I. R., Kindermann, M.... Pick, H., Vogel, H., & Johnsson, K. (2003). A general method for the covalent labeling of fusion proteins...

...not right for the development of phage-based therapeutics. After a brief stint at the venture capital ...Novophage's focus. The company went from developing therapeutics to developing phage-based tools for detecting...develop tests for food products as opposed to therapeutics 15:15 - 16:26 : Means for testing for pathogens...for Dr. Koeris to transition from developing therapeutics to developing a diagnostic? 36:53 - 38:00 : ...

...are required to ensure that you get full library coverage in pooled library screens. Availability and Use...metalloproteinase-9 secretion, and identifying lysine methyltransferase substrates. We’re incredibly happy that lentiCRISPR...of the many plasmids being distributed to and accelerating research in labs all around the world. We thank...Browse Our CRISPR Posts Learn about Using CRISPR to Generate Knockouts Read Some Common Ways to Deliver CRISPR...

...of different conformations and thus potentially interact with and alter the proteins they label. In fact...chaperone Ydj1, both of which display sophisticated interactions with multiple other proteins. Again, little ...indicating that they compromised protein-protein interactions. If you’re looking to a tag a protein, inntags...tags of choice for examining protein-protein interactions, particularly in single-molecule or high-throughput...

... the key component of most CRISPR systems and generates double strange breaks at target sites 5' of the...your 20 nucleotide guide sequence of choice (for generation of a gRNA that will target SpCas9 to the approrpriate... expression post transfection by western blot. Overall, this is a versatile mammalian expression vector...Browse Our CRISPR Posts Learn about Using CRISPR to Generate Knockouts Read Some Common Ways to Deliver CRISPR...

...DayCon. DayCon is a one-day conference aimed at the general public that provides an accessible introduction...conference-style experience that was accessible to the general public both in terms of cost and content level....of disease, cancer, and viral infections. An interactive lunch with student presenters and board members... of shorter, more narrowly focused lectures, interactive panels, and a student poster session. All DayCon...

...available at Addgene, also gives the option to stack several expression cassettes onto a single T-DNA in subsequent...rounds, dramatically reducing the time required to generate multi-construct transgenic plants. In the most...choice, BsaI, which can be very sensitive to temperature fluctuations. He says their lab learned the hard...into the public domain in the process. “We had several motivations for making GreenGate available through...

...towards the objective is collected and focused onto a camera for visualization. Illuminating a sample in the...is excited at one time and captured in a single camera exposure. This is much faster than relying on raster...fast, sensitive detector such as a scientific CMOS camera. If plasma membrane dynamics are the main focus... excitation occurs through the fluorophore’s interaction with an evanescent energy field. Lightsheet microscopes... to a higher photon flux via the more efficient camera detector and lack of a light-reducing pinhole. ...

...s how science progresses after all, with each generation of scientists standing on the shoulders of their...Technology Policy issued a memorandum calling on Federal agencies with over $100 million in annual R&D expenditures...support increased public access to the results of federally-funded research. 2)      In June, the White House...Bohannon published an article describing a sting operation in which he submitted a phony paper to a list ...

...then formulate a plan to further develop your transferable skills and reach your career goals.      Although...subject matter discussed at the meeting, but the overall tone of the meeting is almost more important. As...Wunderlich Z, Estrada J, Park J, Bragdon MD, Lopez Rivera F, Biette KM, DePace AH. Mol Cell. 2015 Jun 4;58... What Makes a Good Manager Anyway Developing Transferable Skills During Science Training ...