We narrowed to 672 results for: FIE

1. Plasmids 101: E. coli Strains for Protein Expression

   Type

   Blog Post

   ... as inclusion bodies, and can be recovered and purified using a special protocol. Alternatively, reducing...

2. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

   Type

   Blog Post

   ...regards to integration, and how researchers have modified them to prevent this integration.  Lentiviruses...

3. Antibodies 101: Beyond Surface Labeling

   Type

   Blog Post

   ...done on live cells. For cellular proliferation, esterified dyes that can enter cells freely are typically...

4. CRISPR 101: Homology Directed Repair

   Type

   Blog Post

   ...with homologous DNA to the target sequence to be modified. In general, the insertion sites of the modification...

5. Hot Biosensors 2022: Year-End Roundup

   Type

   Blog Post

   ...vectors (here and here).  Figure 3: JEDI-2P was identified from a high-throughput screen, selected for increased...

6. Transferable Skills Guide: Conflict Resolution

   Type

   Blog Post

   ...compromise could leave everyone feeling vaguely unsatisfied. In arbitration, there is more of a winner and...

7. Filming Science Videos in the Age of Social Distancing

   Type

   Blog Post

   ...finally found my footing with the company. I’d identified the ways to get the most out the resources we...

8. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

   Type

   Blog Post

   ... we tested a strategy that was discussed in the field at the time but had never been systematically analyzed...

9. March for Science

   Type

   Blog Post

   ...evidence of climate change, the safety of genetically modified organisms (GMOs), the absence of a link between...

10. Five Popular Model Organisms

    Type

    Blog Post

    ...quiescent and proliferative pancreatic β-cells identified by HOTcre mediated labeling." Proceedings of ...

11. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type

    Blog Post

    ... The localization of the fusion protein can be verified and it can be compared to what is expected based...

12. Five Popular Model Organisms, Part 2

    Type

    Blog Post

    ... Science is a constantly evolving and demanding field, requiring a variety of biological and molecular...

13. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type

    Blog Post

    ...cloning of your gRNA of interest. Figure 1: Simplified schematic of agrobacterium-mediated transformation...

14. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type

    Blog Post

    ...worldwide. As we’ve previously seen in the CRISPR field, the lessons learned in pursuing one technological...

15. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type

    Blog Post

    ...Gibson assembly. In this case the vector was PCR amplified to contain regions of homology with the bacterial...

16. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type

    Blog Post

    ...Tabebordbar et al. show that muscle stem cells are modified by this approach, increasing the possibility that...

17. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type

    Blog Post

    ...fluorescent proteins, with recombination events specified by three loxP variants, but the principles remain...

18. Plasmids 101: Common Lab E. coli Strains

    Type

    Blog Post

    ... Inactivating mutations in specific genes are signified with a minus sign (-) as is typically standard...

19. AAV Production in HEK293 Cells

    Type

    Protocol

    .... You are now ready to purify your prep . The clarified supernatant can be kept overnight at 4 °C before...

20. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type

    Blog Post

    ...there are up to thirteen natural serotypes of AAV identified so far, numbered AAV1, AAV2, and so on. In addition...