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We narrowed to 665 results for: FIE

Showing: 621 - 640 of 665 results

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Filming Science Videos in the Age of Social Distancing

Type

Blog Post

...finally found my footing with the company. I’d identified the ways to get the most out the resources we...
Five Popular Model Organisms

Type

Blog Post

...quiescent and proliferative pancreatic β-cells identified by HOTcre mediated labeling." Proceedings of ...
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

Type

Blog Post

... The localization of the fusion protein can be verified and it can be compared to what is expected based...
March for Science

Type

Blog Post

...evidence of climate change, the safety of genetically modified organisms (GMOs), the absence of a link between...
CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

Type

Blog Post

...cloning of your gRNA of interest. Figure 1: Simplified schematic of agrobacterium-mediated transformation...
CRISPR 101: Homology Directed Repair

Type

Blog Post

...with homologous DNA to the target sequence to be modified. In general, the insertion sites of the modification...
Antibodies 101: Beyond Surface Labeling

Type

Blog Post

...done on live cells. For cellular proliferation, esterified dyes that can enter cells freely are typically...
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

Type

Blog Post

... we tested a strategy that was discussed in the field at the time but had never been systematically analyzed...
Hot Biosensors 2022: Year-End Roundup

Type

Blog Post

...vectors (here and here). Figure 3: JEDI-2P was identified from a high-throughput screen, selected for increased...
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

Type

Blog Post

...worldwide. As we’ve previously seen in the CRISPR field, the lessons learned in pursuing one technological...
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

Type

Blog Post

...Gibson assembly. In this case the vector was PCR amplified to contain regions of homology with the bacterial...
Treating Muscular Dystrophy with CRISPR Gene Editing

Type

Blog Post

...Tabebordbar et al. show that muscle stem cells are modified by this approach, increasing the possibility that...
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

Type

Blog Post

...fluorescent proteins, with recombination events specified by three loxP variants, but the principles remain...
Plasmids 101: Common Lab E. coli Strains

Type

Blog Post

... Inactivating mutations in specific genes are signified with a minus sign (-) as is typically standard...
How to Design Your gRNA for CRISPR Genome Editing

Type

Blog Post

...modification. Whole-genome sequencing of cells modified by CRISPR indicates that the consequences of off-target...
Antibodies 101: The Basics of Western Blotting

Type

Blog Post

...sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with...
Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

Type

Blog Post

...researchers to share data thereby enabling you to do modified analyses on published data if you need to. Similarly...
Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

Type

Blog Post

... that it requires a high-concentration, column-purified DNA template. Many thanks to Channabasavaiah...
Viral Vectors 101: AAV Variables That Matter

Type

Blog Post

..., and 9 in two different brain regions. Image modified from Aschauer et al., 2013, Figure 1. Used under...
Viral Vectors 101: AAV Serotypes and Tissue Tropism

Type

Blog Post

...there are up to thirteen natural serotypes of AAV identified so far, numbered AAV1, AAV2, and so on. In addition...

Showing: 621 - 640 of 665 results

Results per page:

Filming Science Videos in the Age of Social Distancing

Five Popular Model Organisms

Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

March for Science

CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

CRISPR 101: Homology Directed Repair

Antibodies 101: Beyond Surface Labeling

3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

Hot Biosensors 2022: Year-End Roundup

Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

Treating Muscular Dystrophy with CRISPR Gene Editing

Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

Plasmids 101: Common Lab E. coli Strains

How to Design Your gRNA for CRISPR Genome Editing

Antibodies 101: The Basics of Western Blotting

Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

Viral Vectors 101: AAV Variables That Matter

Viral Vectors 101: AAV Serotypes and Tissue Tropism

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