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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 856 results for: HA

Showing: 621 - 640 of 856 results

  1. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...target is known to be absent naturally or in which it has been knocked-out/knocked-down. “No primary”, pre-... reliable in the future.  We hope that this post has shed some light on immunofluorescence. The technique...

  2. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...Cytotoxicity can also be cell type dependent: if a cell has few receptors for a specific envelope protein, it...lentiviral vector to be transported retrograde and also has higher neuronal transduction efficiency and a better...

  3. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...editing systems in bacteria. Our pCas/pTargetF system has been one of the most popular genomic editing systems...Yang lab.   In conclusion, the MUCICAT system has the following advantages: Fast and site-specific...

  5. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ...high for both plasmids, so each plasmid “thinks” it has a higher copy number than it actually does, and neither...large, low copy virulence plasmids. This strategy has been successful in displacing, or “curing” the virulence...

  6. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...lab to split my cells later. Eating with friends has a somewhat immediate deadline - even the best of ...can be quite difficult. In my experience, planning has 3 components: A mechanism for blocking off time ...

  8. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...instances where it does. 1) When the backbone vector has multiple BbvCI sites. If these sites have opposite...example like this. 2) When your gene of interest has a BbvCI site(s). If so, the nicking mutagenesis protocol...

  10. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...mycoplasma contamination. Mycoplasma contamination has been shown to arise from a variety of sources such...line for mycoplasma and discard it if the culture has become overly confluent or too old. Ensuring that...

  11. Addgene's Website Update

    Type
    Blog Post
    ...soon as the homepage opens. You’ll notice it now has two options: “Search Repository” and “Deposit Materials...

  14. CRISPR Library Amplification

    Type
    Protocol
    ... evenly with a sterile spreader until all liquid has been absorbed by the agar. This usually takes 1-2...
Showing: 621 - 640 of 856 results