We narrowed to 877 results for: HA

...fact, they’ve been following a set of clues that has led them to their biggest discovery yet: Addgene ...

...at Missouri University of Science and Technology, has been teaching the microbiology lab every year for...

...newest viral vector service, AAV Packaged on Request  has seen a fantastic response from the research community...

...multiple sites in cultured human cells. While it has not yet been demonstrated, they say the vectors are...

...technology and rapid improvements in the field, it has recently become possible to introduce DSBs and concomitant...efficiency across different stem cell types. This has facilitated easy generation of clonal knock-in lines...or try out an alternative tag (3xFLAG, Myc, V5, or HA). Tag-specific PCR genotyping can tell if HDR completely...

...as they pass our standard QC process and the MTA has been approved. The spreadsheet option is recommended...cloned into the vector backbone). If your plasmid has more than one insert, click "Save and Add Another...primers can be used to verify it. If your plasmid has multiple inserts, enter details for each insert individually...

...combining the function of both editors into one? This has been done by fusing the adenine and cytosine editing...deaminases with a Cas9 nickase (Zhang et al., 2020). It has increased CBE activity and reduced RNA off-targeting...engineering and directed evolution of the TadA domain has yielded base editors that are equally efficient at...which uses a different mechanism than base editing, has allowed scientists to replace entire sequences of...

...where she studied diabetes and metabolism. Melina has worked at Genetics Institute in Cambridge, MA and...

...therapeutics to treat neurodegenerative diseases. He has earned a B.S. in microbiology and subsequently obtained...

...takes to make one plasmid and how much time Addgene has saved researchers. Assuming 5 hours of hands-on time...

... and microbe cookies! 🤗 🤗 🤗 We hope everyone has a safe and festive Holiday season. 🙌 🥂 🎄 🎉 😷...

...Alex is the first MD PhD we've had on the podcast, has worked extensively with CRISPR, and recently became...

... of excitement at Addgene. Our new viral service has begun to pick up steam (we already have over 70 orders...

...2004 to today, you can see how plasmid distribution has grown. Additionally, more and more scientists outside...

...bridge helix. Created with BioRender.com.   Cas9 has two major lobes, called NUC (for nuclease) and REC...gray). Target DNA strand, light gray.   Once it has found a PAM site, Cas9 needs to check for complementarity...orange) and HNH (red) domains, while the REC lobe has been hidden for visibility. A RuvC loop caps the ...

...these adaptive bacterial and archaeal immune systems has led to extreme sequence divergence that requires ...landmark publication in 2013. Since that point, it has become the most engineered Cas enzyme, with adaptations... CRISPR type that exclusively targets RNA. Cas13 has become a natural successor to traditional RNA interference...

...lab to split my cells later. Eating with friends has a somewhat immediate deadline - even the best of ...can be quite difficult. In my experience, planning has 3 components: A mechanism for blocking off time ...

... virulence (Tollefson et al., 1996) – cell death has been reported when using vectors, and immune inactivation...the infection to sometimes result in cell death, has led to interest in using adenoviral vectors for vaccine...

...eBook, How to Get a Faculty Position. This edition has substantial expansions on the chalk talks and Zoom...I also incorporated example documents. This part has generated a lot of positive feedback. Many of these...

...applicable in DSB-induced genome engineering, it has biological importance for the repair of breaks that...directed repair and genome engineering CRISPR-Cas9 has revolutionized the genome engineering world and made...