We narrowed to 771 results for: LIS

1. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

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   ...negligible level. One major reason we chose to publish the concept and likely capabilities of RNA-guided...

2. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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   ...purification; the whole cloning process is so simplistic once you have your parts. That was the motivation...

3. Tips for Using BLAST to Verify Plasmids

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   ...alignment tools available. Try these resources for lists of alternatives to BLAST: ExPASy - http://www.expasy.org...

4. Which Fluorescent Protein Should I Use?

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   ...pH changes (e.g. pHluorin, pHTomato). Keep this list handy as you plan your next experiment or to hand...

5. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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   ...genome engineering in bacteria, alongside more established tools such as Cas9-recombineering, CRISPRa/i,...

6. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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   ...embed_player" overrideable=False, type='scriptV4', hide_playlist=True, viral_sharing=False, embed_button=False...

7. Summer SciComm Series: Modes of Communication

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   ...answered the above questions, you can create your short list of options and then pick whichever is most accessible...

8. Plasmids 101: Choosing an Antibiotic Resistance Gene

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   ... toxic to dividing cells. The other antibiotics listed below affect continuous cellular processes (e.g...

9. DNA Purification Without a Kit

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   ...adapted from the 1979 Patterson Protocol. When published, the Patterson Protocol was shown to purify DNA...

10. Droplet Digital PCR for AAV Quantitation

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    ... of parameters influencing droplet rain in the Listeria monocytogenes prfA assay-reduction of ambiguous...

11. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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    ... selected and ranked based on the previously established sgRNA rules and  potential on- and off-target...

12. Plasmids 101: Colony PCR

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    ...restriction enzyme digest; however colony PCR can accomplish the same thing in less time and for less money...

13. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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    ... the viral mRNAs and minigenome. This can be accomplished in multiple ways: transfecting with an additional...

14. Tips for Screening with Yeast Two Hybrid Systems

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    ...to yeast transformation protocols also helped establish the yeast two-hybrid (Y2H) system as a robust ...

15. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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    ...grant situation. So, young researchers need to publish good publications in a short period of time and...

16. Hot Plasmids - August 2020

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    ...mTurquoise2, mNeonGreen, and mScarlet-I for labeling a list of specific structures within cells. For example...

17. Plasmids 101: Plasmid Incompatibility

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    ...2012) Bacterial plasmid partition machinery: a minimalist approach to survival. Current Opinion in Structural...

18. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

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    ...safe-targeting guides as a control will result in a smaller list of true positive hits than when using non-targeting...

19. Neuronal labeling with Spaghetti Monster

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    ...appear to be very effective probes for finely visualising sub-cellular structures or low-abundance proteins...

20. Analyzing CRISPR Editing Results with ICE from Synthego

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    ... a graphical representation of the results and a list of the analyzed samples (see below).   If the sample...