Lab Automation at Addgene
Type
Blog Post
... of pushing them in one by one. It seems very simplistic and it isn’t electronic but it is one of the ...
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Tips for Technical Support Calls
TypeBlog Post...take to get an answer, consider asking to avoid unrealistic expectations. And finally, provide feedback... -
Gaining Leadership Skills Volunteering at a Professional Organization
TypeBlog Post...were held during the weekend. I attended multiple CLIs and benefited greatly by improving my skills and... -
Experimenting in an Open Source lab: from CRISPR to Cats
TypeBlog Post...makerspaces around the world. Hackerspaces.org maintains a list of active spaces near you. Learning about C. ... -
Hot Plasmids and Viral Preps - May 2021
TypeBlog Post...synthetic human scFV library and characterized them by ELISA, homogeneous time resolved fluorescence, and suspension... -
3D Printing Meets CRISPR Cas9
TypeBlog Post...could make a model of Cas9 based on the structure published by Martin Jinek’s group. We had already made an... -
Cloning Mammalian Cells with the Agarose Method
TypeBlog Post...protein, we have used Western blots, dot blots, ELISAs, and enzyme assays to select both over- and under-expressing... -
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies
TypeBlog Post... stocks that are available to everyone. Flybase lists what stocks are available at Bloomington, but you... -
The AAVantages of AAV in CRISPR Screens
TypeBlog Post..., M., Farouni, R., Lagler, S., Kalamakis, G., Lischetti, U., Jabaudon, D., & Platt, R. J. (2023). Transcriptional... -
Common Injection Routes in Mice
TypeBlog Post... for you — and the mice. While not an exhaustive list, these can include what’s being injected, the type... -
Production of Virus in Insect Versus Mammalian Cells
TypeBlog Post...Tompkins, G. J., & McCawley, P. (1977). The establishment of two cell lines from the insectspodoptera ... -
Magnetic Control of Proteins: More than a Dream
TypeBlog Post...range of topics. He embraces open science, self-publishing directly on his website (and often posting updates... -
Transferable Skills Guide: Managing a Team
TypeBlog Post... Equally as important is to be able to actively listen - this can be hard - but it is the best way to ... -
Addgene's Cancer Collection Pages Connect the Research Community
TypeBlog Post...gene of interest will direct the investigator to a list of all available Addgene plasmids he or she can ... -
Plasmids 101: Optimizing Plasmid Yields
TypeBlog Post...handy guide from OpenWetWare for a more extensive listing of common E. coli strains. Increase the volume ... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog Post...dimeric FP could ruin your day (and experiment) are listed below. Solutions to avoid these all too common ... -
Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards
TypeBlog Post...negligible level. One major reason we chose to publish the concept and likely capabilities of RNA-guided... -
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More
TypeBlog Post...purification; the whole cloning process is so simplistic once you have your parts. That was the motivation... -
Tips for Using BLAST to Verify Plasmids
TypeBlog Post...alignment tools available. Try these resources for lists of alternatives to BLAST: ExPASy - http://www.expasy.org... -
Which Fluorescent Protein Should I Use?
TypeBlog Post...pH changes (e.g. pHluorin, pHTomato). Keep this list handy as you plan your next experiment or to hand...