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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 851 results for: Met

Showing: 621 - 640 of 851 results

  1. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...companies have dedicated technical support teams, sometimes comprised of several layers of support: A front-line...listen to the many individual stories and get (sometimes refreshing) insights into how other scientists...

  2. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...editors, keep in mind that the generation of indel sometimes may also results in RE site destruction. Many... editing in rice and uses base editors and HDR methods for rice crop improvement in the laboratory of ...

  5. Hot Plasmids - November 2023

    Type
    Blog Post
    ..., then the other, a dual-enrichment and mass spectrometry workflow identifies proteins bearing both labels... of proximity labeling in living cells. Nature Methods, 20(6), 908–917. doi: 10.1038/s41592-023-01880-...

  6. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ..., or pretty much anyone applying the scientific method to a question. Videos from any discipline are welcome...the University of Maine, about her research on plummeting embryo survival rates. Video on We Share Science...

  8. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...Tends to resist promoter down regulation due to methylation or deacetylation. Ubc General expression mRNA... General expression Promoter of the arabinose metabolic operon Inducible by arabinose and repressed catabolite...

  9. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...reservoirs, chronic inflammation, and negative metabolic effects of ART. Why can’t the body mount an immune...Obstacles for CRISPR HIV-1 therapy Both of these methods represent exciting advances in preclinical HIV-...

  10. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...equipment (RT-PCR, Microscopy, Fluorescence Scopes, Flowcytometers, etc.). After identifying all the major equipment...their success. Therefore, assume positive intent. Sometimes, “no we don’t have the funds,” really means they...

  11. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...technology, and the user may need to optimize numerous parameters to generate the desired edit. The wrench: Gene...universal scoring system for selecting a gRNA, as the method of producing the guide (synthetic, in vitro transcription...

  13. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...milestone for the molecular biologist was to devise methods to manipulate it. The term genetic engineering ...history of CRISPR Read about other genome editing methods Resources on Addgene.org Visit our CRISPR guide...

  14. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ... constructs that compose genetic circuits and metabolic pathways. For many MoClo systems, the individual...interested in recombinant protein purification and metabolic engineering. If instead you want to build genetic...

  15. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...through multiple cell divisions, indicating that this method produces stable edits. However, this system is ... effects, ABEs also compare favorably to other methods. In a head-to-head comparison with Cas9, Cas9 modified...
Showing: 621 - 640 of 851 results