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We narrowed to 882 results for: NSI

Showing: 621 - 640 of 882 results

  1. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ... capture large field-of-views (6). For more comprehensive reviews on several other LSFM configurations...Bradke laboratories later developed the 3DISCO (3-dimensional imaging of solvent cleared organs) method of ...

  3. Lentivirus ddPCR Titration

    Type
    Protocol
    ...bleach. Safety Warnings Lentivirus is generally considered biosafety level 2+. Please ensure that you are... media and 11.1 µg/mL Polybrene. Mix the cell suspension well before seeding. Mix each well with a 1 mL...45° angle. Count to 20 while slowly and gently dispensing the droplets down the side of the tube. Place... 95 10 2 1 Denaturation 94 0.5 2 40 Annealing/Extension 60 1 2 40 Enzyme Deactivation 98 10 2 1 Hold 4...

  4. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...non-dividing cells. The first lentiviral vector system consisted of three plasmids: the packaging, envelope, and...capacity of a lentiviral vector but for which transient transgene expression is sufficient. Lentiviral...

  6. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...students. But he also found that his teaching responsibilities left him with limited time for one-on-one ...notes the importance of acknowledging student apprehensions about “failure” (protocols not working, hypotheses...

  7. Tips for arabidopsis transformation

    Type
    Blog Post
    ...that is wide enough to dunk your plant’s flowers inside. 50 mL falcon tubes or plastic weigh boats are ...from a colony on a plate will reach an optimal density in 18-24 hours. I then add 0.5 mL of this starter...

  10. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...enzymes do not contain the RuvC and HNH domains responsible for DNA cleavage, so they cannot directly edit.... PspCas13b does not require a PFS, and it is sensitive to mismatches in target RNA from bases 12-26 of...

  12. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...For these reasons, we are in the process of transitioning our titering method from qPCR to ddPCR. Droplet...titer your AAV by ddPCR. Droplet digital PCR is a sensitive assay, so cross contamination into a no template...

  14. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...connections between biology and ancestral groups or, by extension, race. Many researchers do large-scale CRISPR ...potential for bias but to actively look for and consider bias as a confounding factor when interpreting...

  15. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...Saccharomyces cerevisiae in 1989 and are still used extensively to screen for molecular interactions in the cell...post-translationally modified, co-expression of the enzyme responsible for the modification in the assay host strain...

  17. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...technical roles. Therefore, institutions are responsible for ensuring that either all affected scientists...and Brandeis universities have stated that they consider postdocs paid on stipends to be FLSA exempt, and...

  19. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...d like to manipulate in mind. Now it’s time to consider where you would like to attack this gene. Should...sequences into HEK293T cells. The fluorescence intensity was classified into four groups (4; brighter than...

  20. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ...with BioRender.com. Sanger sequencing was considered the gold standard until about 2008 when a new...novo assembly within Geneious gives us additional insight into the assembly. Geneious will show how reads...
Showing: 621 - 640 of 882 results