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We narrowed to 1,023 results for: NTS

Showing: 621 - 640 of 1023 results

  1. Science as Art

    Type
    Blog Post
    ...following the trend and encouraging departments to showcase the work of students and staff by holding scientific...method to explore the inner structures of delicate plants and other organisms without destroying the original...organisms on your lab bench? Let us know in the comments.    Additional Resources on the Addgene Blog ...

  2. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    ... is a database that provides information about reagents, funding and volunteer opportunities, protocols... hopefully help guide policies and ultimately treatments in a timely fashion.”  With this in mind, Bourque...including datasets and platforms for discussing new developments, and more. As researchers around the world continue...community work together to share open protocols, reagents, and other initiatives for #COVID19 research. ...

  3. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

    Type
    Blog Post
    ...and hide individual vectors (shapes defined by 2D points connected by lines and curves) that you’ve drawn...Layers are useful for drawing figures with lots of elements. It took some playing around to figure out all...images to the Biorender workspace. Free Biorender accounts can store 5 workspaces at a time and workspaces...resource not mentioned? Tell us about it in the comments section!  Additional resources on the Addgene...

  4. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...forms of antibodies and had begun developing Fab fragments and scFvs. But the potential for an even smaller... they compare to other antibodies and affinity reagents. But for a quick refresher, some of the advantages...target concave epitopes Suitability for in vivo experiments, due to their simple structure    sdAbs in research... camelid mice and llamas neutralize SARS-CoV-2 variants. Nature 595:278–282. https://doi.org/10.1038/s41586...

  5. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type
    Blog Post
    ...peak absorption wavelengths and light intensity requirements of different opsins, studies have shown that...generates periodic motor commands for rhythmic movements like spontaneous swimming. To do so, the lab expressed... behaviors, the researchers measured the tail movements of 5 day old fish. The larvae were immobilized... groups of neurons that elicited similar tail movements, they were able to narrow down the possible specific...

  6. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ... in vertebrates. The system is comprised of 2 components: 1) an SB transposase, the enzyme required for... and modifying various extinct transposases. Improvements have been made since then by increasing the ...Zsuzsanna Izsvak’s lab screened many SB tranposase variants for hyperactivity in mammalian cells. Their efforts...inducible expression vectors were developed into 16 variants with different selection markers (blasticidin,...

  7. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    ...Bozeman Sunday night, it was easy to spot meeting participants. They were the ones holding poster tubes (or...Zhang's introduction. His willingness to share reagents so freely with the academic community has clearly...CRISPR delivery systems, modifications to the RNA components, and challenging target genomes, such as the ...who contact us and want a guarantee that their experiments will work (yes, this happens): Science is hard...

  8. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ...antibiotics? Antibiotics are generally defined as agents that kill bacteria, or inhibit their growth. Although...be a bit of a gray area as some bacteriostatic reagents can kill bacteria when used at high concentrations... the mutation. When designing these types of experiments it is best practice not to use the same resistance...Blue White Screening to Simplify Your Cloning Experiments See Where You Can Find Antibiotic Resistance ...

  9. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    ...mammalian cells, likely due to high temperature requirements. To solve this problem, Gao et al. searched ...potential for off-target effects. In Gao et al.’s experiments, a single mismatch at any position of the DNA...using NgAgo? Let us know how it’s working in the comments section! Read About NgAgo User's First Impressions... Cas9 Homolog Cpf1 Check Out High Fidelty Cas9 Mutants Browse All Genome Engineering Posts Additional...

  10. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type
    Blog Post
    ...error rates compared to other RTs. MarathonRT represents a major step forward in facilitating techniques...understanding thereof have the potential to provide improvements to existing technologies based on polymerase...segment Nanobodies are single domain antibody fragments typically engineered from heavy-chain only antibodies... C-BERST (dCas9–APEX2 biotinylation at genomic elements by restricted spatial tagging), to confirm the...

  11. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    .... In neutral environments, dimeric Keima is excited at 440 nm and in acidic environments at 586 nm. Regardless...mechanism as Rosella: GFP is quenched in acidic environments, while mRFP is relatively stable and fluorescences...18094608. Sargsyan, A., et al. "Rapid parallel measurements of macroautophagy and mitophagy in mammalian...

  12. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...if we can help them share and distribute other reagents. Virus distribution came up time and time again...this frustration and getting researchers to the experiments they’re interested in faster by producing, titering...you out. Of course, lentiviruses are used in experiments other than just those involving CRISPR and so...first two can serve as controls in a variety of experiments, while rtTA can be used to activate expression...

  13. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ...origin of replication (ori) and all of its control elements. The ori is the place where DNA replication begins...lot of your plasmid? Is the gene toxic in high amounts? It is always good to keep in mind that plasmids... medium copy numbers can still express massive amounts of protein given the proper promoter and growth...something clarified? If so please, post in the comments.  References Chatterjee A, Sun SB, Furman JL, ...

  14. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    ...selection strategy for their C. elegans/CRISPR experiments, but this strategy required the use of unhealthy...Other labs have used PCR-based screening to detect mutants, but this method limits screening to a few hundred...hundred worms, making the detection of rare events difficult. Dickinson et al. sought to create a new and.... This novel, rapid, and user-friendly system represents another leap forward for the CRISPR/Cas9 revolution...

  15. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ...Presentation Experience”, “Career Achievements”, and “Academic Achievements”. Leaning my CV more towards me... business friendly CV that would focus on my achievements, capabilities, courses and office skills. I ...matched it with my CV and added more business-like elements. At this point you may be asking yourself, "Why...

  16. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ... to treat human genetic diseases, and several treatments are currently being tested in clinical trials...recombination in cultured intestinal stem cells of patients with cystic fibrosis (8).   The difference with...correction in adult stem cells isolated from two patients with cystic fibrosis. They were able to demonstrate...Intestinal Stem Cell Organoids of Cystic Fibrosis Patients." Cell Stem Cell. 13, 653-658 (5 December 2013...

  17. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type
    Blog Post
    ...first sent a pre-workshop questionnaire to the participants to learn about what they were looking to learn... content. In the middle of the workshop, the participants took part in a protocol drawing exercise. The...workshop also used a shared note-taking doc where participants could share their notes together. Figure...viewed publicly and stored in one place if the participants used the conference hashtag after the conference...

  18. Science Career Options

    Type
    Blog Post
    ...When preparing to graduate from university, many students are confronted with the question ‘what now?’ This...sectors such as forensics currently have too many applicants compared to the number of available roles). Make...useful to search online job websites to see what requirements or qualifications are needed for the role, as...you get your foot in the door. Match the job's requirements to the content in your Resume and Cover Letter...

  19. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ...others to see what you actually found, to providing reagents in an accessible and consistent way so other researchers...methods sections, including clear information about reagents, and why other publishers and organizations have...in their communication or making their data and reagents readily available. What if that could change? ...scientific enterprise will benefit. We’ll have fewer students and postdocs sitting frustratedly in reproducibility...

  20. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    ... the fluorescent toolbox Modern neuroscience experiments often rely on multiplexing, where multiple fluorescent...green, yellow, and/or red sensors in multicolor experiments. With OCaMP, researchers can image multiple brain...F0 (the measure of signal change during calcium events), Greater brightness, Less signal distortion from...above. The number of action potential (AP) spiking events are indicated below the trace; asterisk indicates...
Showing: 621 - 640 of 1023 results