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We narrowed to 114 results for: NTS

Showing: 1 - 20 of 114 results

  1. CRISPR Plasmids - Plants

    Type
    Collection
    ... CRISPR Plants CRISPR Plasmids: Plants Browse CRISPR Plasmids By Function...have been designed for use in plants. CRISPR...following CRISPR plasmids have been designed for use in plants. Cut Fully functional CRISPR/Cas enzymes will introduce... Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand breaks in...strategies reduce unwanted off-target effects. Nickase mutants can also be used with a repair template to introduce...

  2. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...Phosphoprotein Reagents Rinehart Lab Phosphoprotein Reagents Phosphoprotein Synthesis Reagents References ... for Improved 2015 Phosphoprotein Reagents (DOCX, 525.9 KB) Reagents (ca. 2014) described in: Heinemann...Protocol for Reagents (ca. 2014) from the Rinehart lab: Users Guide for 2014 Phosphoprotein Reagents (PDF, 691.3...the Rinehart and Issacs labs at Yale have made improvements to this system by genomically recoding E.coli...system, pSerOTS-C1* (V70) (Plasmid #188537) . These reagents are described in detail in Mohler et al. (2023...for laboratories performing phosphoproteomic experiments in need of reference spectra or phosphopeptide...phosphopeptide standards for quantitative assessments. The Mode #1 phosphosite library can be generated with either...

  3. Zinc Finger Consortium Reagents

    Type
    Collection
    ...describing these reagents are provided below: Zinc Finger Genome Engineering Reagents Zinc Finger Arrays...OPEN) Reagents - Engineer zinc finger arrays via OPEN method Modular Assembly Engineering Reagents - Engineer...Find reagents for engineering and expressing zinc finger proteins deposited by Zinc Finger Consortium... Zinc Finger Consortium Zinc Finger Consortium Reagents You may also like... Keith Joung Lab plasmids ...Daniel Voytas have deposited at Addgene various reagents for engineering and expressing zinc finger proteins...Genome Engineering Technologies TALEN Engineering Reagents CRISPR Plasmids...

  4. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...function in zebrafish, plants, and human cells... Kits Zinc Finger Consortium OPEN Reagents Zinc Finger Consortium...Consortium: OPEN Reagents (Kit # 1000000013 ) Depositing Labs: Keith Joung This kit consists of plasmids...plasmids Detailed Information Protocols & Resources Contents of Kit Original Publication Oligomerized pool ... request from the Joung lab) and the following reagents (listed below and available individually or as...as an “OPEN Accessory Reagent Set”). These reagents are also valuable research tools for using the bacterial...used to engineer ZFNs that function in zebrafish, plants, and human cells ( Maeder et al., Mol Cell 2008...

  5. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...Kleinstiver BP, Pattanayak V, Prew MS, Tsai SQ, Nguyen NT, Zheng Z, Joung JK. 2016. High-fidelity CRISPR-Cas9...Kleinstiver BP, Prew MS, Tsai SQ, Topkar VV, Nguyen NT, Zheng Z, Gonzales AP, Li Z, Peterson RT, Yeh JR,...specialized Cas proteins snip foreign DNA into small fragments approximately 20 bp in length and paste them into...These edits can include mutations seen in human patients, protein tags, or loxP/FRT sites, among others... High fidelity Cas9s: Three high fidelity Cas9 variants , SpCas9-HF, eSpCas9, and HypaCas9 display very...Streptococcus , Streptomyces , and others) Drosophila Plants (monocots and dicots) C. elegans Yeast ( S. cerevisiae...

  6. Immunology Research Plasmids and Resources

    Type
    Collection
    ...NTF4 neurotrophin 4 NT-4/5, NT4, NT5, NTF5 NTS neurotensin NMN-125, NN, NT, NT/N, NTS1 NUDT6 nudix (nucleoside...Antibodies produced by B cells activate immune components that also lead to the destruction of pathogens...

  7. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...GRAB biosensors for neuropeptides VIP, NPY, CCK, NTS, UCN, SST, and CRF A tool kit of highly selective...Quantitative measurements of membrane charges A FRET sensor enables quantitative measurements of membrane...designed to specifically localize to cellular compartments or organelles. Addgene’s database includes many...that many of the articles linked below include variants with different binding affinities or negative-...imaging activity in neuronal populations and microcompartments. Nat Methods. 2019 Jul;16(7):649-657. Douglas...astrocytes (GCaMP6f), cytosolic Conditions and constraints for astrocyte calcium signaling in the hippocampal... Calcium Yellow protein calcium sensor jYCaMP1 variants (Constitutive or Cre-dependent) jYCaMP: an optimized...

  8. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ... made up of microtubules, actin filaments, and intermediate filaments. These structures give the cell ...GFP-cortactin Actin Filaments Cortactin EGFP Anna Huttenlocher 32907 pEGFP-rNFM Intermediate Filaments (neuronal...Intermediate Filaments (neuronal) Nefm EGFP Anthony Brown 11908 pEGFP-N1 alpha-actinin 1 Actin Filaments alpha-actinin...Actin Filaments Cortactin mCherry Christien Merrifield 36201 pLifeAct-mTurquoise2 Actin Filaments LifeAct...pLifeAct_mScarlet_N1 Actin Filaments LifeAct mScarlet Dorus Gadella 85055 pLifeAct_mScarlet-H_N1 Actin Filaments LifeAct ... Actin Filaments ITPKA mTurquoise2 Dorus Gadella 58473 pEGFP-C1 F-tractin-EGFP Actin Filaments F-tractin...mCherry-Lifeact-7 Actin Filaments LifeAct mCherry* Michael Davidson 54610 mEGFP-Lifeact-7 Actin Filaments LifeAct EGFP...

  9. Antibody Guide

    Type
    Collection
    ...antibody fragments consisting of two scFvs bound to a CH3 domain. Fab fragments - Fab fragments are created... called the antigen binding fragments (Fab). The tips of the Fab fragments contain highly variable regions...Affinity Reagents There are several alternatives to antibodies, ranging from antibody fragments (shown ...Structure Isotypes Antigen Specificity Other Affinity Reagents Handling Visualization Signal Amplification Application...Multiplex Assays Selection Validation Affinity reagents are molecular tools used in the lab to specifically...foreign bodies, are the most commonly used affinity reagents. Antibodies are Y-shaped proteins, naturally produced...interest is introduced to the animal, which then mounts an immune response to it. Once the immune response...

  10. Plant Plasmids and Resources

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    Collection
    ...in monocot and dicot plants - Yao-Guang Liu. Plasmids for prime editing in plants (including rice and ...Addgene's plant resources: Find plasmids for use in plants. Plasmid...tools that were specifically designed for use in plants. These resources include tools for research using...useful toolkits and plasmid collections for use in plants. The tables below highlight plasmids spanning a...selection of plasmids expressing different functional variants of Cas9 and empty gRNA backbones. A few examples...- Yinong Yang. The MoClo CRISPR/Cas Toolkit for Plants includes CRISPR/Cas nucleases, base editors, gRNA...assembly for fast generation of multiple Arabidopsis mutants - Niko Geldner. CRISPR-Cas9 vector with GRF4-GIF1...

  11. CRISPR Guide

    Type
    Collection
    ...multiple Cas9 variants, including high fidelity Cas9s, Cas9s with various PAM requirements, and other Cas...scale edits by integrating segments of DNA flanked by specific repeat elements into the genome. CRISPR-associated...mechanism Engineered CRISPR systems contain two components: a guide RNA ( gRNA or sgRNA ) and a CRISPR-associated...mechanisms of CRISPR and some of the ways the CRISPR components are leveraged to achieve specific goals, such...the Cas enzyme. Basic Mechanisms of CRISPR The requirements for a CRISPR knockout assay are simple. You’...Cut) Multiplex Genome Engineering Many CRISPR experiments involve editing more than one gene. In these ...RuvC and HNH, can be altered to reduce cleavage events to a single cut (nick) or no cuts. Cas9 nickase...

  12. Plan Your Experiment

    Type
    Collection
    ...proteins available, and researchers can adapt these experiments for different organisms. This page provides a...CRISPR in your research and setting up CRISPR experiments. We will focus on making single edits using CRISPR...using CRISPR in other organisms. As with most experiments, you first need to have a biological question...you are ready to start designing the specific components (Cas enzyme and guide RNA) for your experiment...genetic manipulations require different CRISPR components. Selecting the genetic manipulation you plan ...plan to perform will help you narrow down which components you’ll need for a given experiment. In the table... window from the PAM sequence, and base editor variants often have different optimal editing windows. ...

  13. Antibody Plasmid Collection

    Type
    Collection
    ...antibody components into plasmids and expressing these in bacteria, mammalian cells, yeast, plants, or insect...size. These recombinant antibody fragments can be used in experiments such as immunoprecipitation and ...antibodies as Fab' fragments, isotype-switched chimeric antibodies, and Fc-silent mutants. Functional diversification...microscopy. Two commonly used plasmid-based antibody fragments are scFvs and single-domain antibodies (also known..., scientists often use plasmids encoding scFv fragments that bind to the GCN4 peptide, from the popular...the generation of chicken monoclonal antibody fragments by phage display. J Immunol Methods. 2000 Aug ...dimerisation and detection of recombinant antibody fragments in the form of scFvs A simple vector system to...

  14. Luciferase Plasmid Collection

    Type
    Collection
    ...luciferase a popular tool for scientists. Regulatory elements such as promoters, enhancers and untranslated ...secreted and more stable, enabling time course experiments, but is too dim for some applications. NanoLuc...Creating ORF-Firefly luciferase gene fusions in plants. Renilla luciferase under the control of a CMV ...white screening can be used for screening of transformants in cloning process. Lynne Postovit 64034 pGL4Luc-RLuc...fragment for split-luciferase complementation assay in plants using Gateway cloning. Use with 174051 Alberto ...fragment for split-luciferase complementation assay in plants using Gateway cloning. Use with 174050 Alberto ...Transcriptional unit for firefly luciferase expression in plants (GoldenBraid 2.0). Diego Orzaez 68215 pEGB 35S:...

  15. Bacterial Expression Systems

    Type
    Collection
    ...bimolecular fluorescence complementation experiments in plants. The Zinc Finger Consortium Modular Assembly...plasmids that can help researchers plan their experiments using bacterial expression systems. Read below...proteins of interest to transcriptional regulatory elements that will turn on gene expression upon protein–protein...generation of FRET biosensors for ratiometric measurements. The MoBiFc Toolkit (Addgene #1000000222) is...Plasmids Reporter plasmids can be used to detect events and molecules in and around a bacterium. Plasmids...Chromoproteins: Colorful Proteins For Molecular Biology Experiments Return to top Genome Engineering Modifying the...cellular resources otherwise spent on producing high amounts of protein. Whether you're investigating basic ...

  16. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...C-terminal fragments and placed under the control of different promoters. Expression of both fragments in the... gene expression, and find plasmids for your experiments. Plasmid...Site Canonical Sequence Structure (Length) Common Variants Cre loxP ATAACTTCGTATAgcatacatTATACGAAGTTAT 13...system allows scientists to utilize recombination elements such as Cre to turn off the expression of one ...combined in multiple, independent recombination events in a single experimental system, enabling multi-layered...lineage-specific drivers can also help reduce off-target events and the systemic effects of Cre toxicity. Leakiness...iCre variant, reduces the number of recombination events but can improve specificity in targeted neuronal...

  17. Lentivirus Plasmids

    Type
    Collection
    ... Guide for more information about: Lentiviral components 2nd versus 3rd generation lentiviral systems ...feature of lentivirus used in research is that the components necessary to produce an infectious viral particle...called packaging and envelope plasmids) encode components of the viral capsid and envelope and are used...transfer plasmids along with additional packaging components Trono 63586 psPAX2-D64V 2nd Packaging For making...that encodes genetic material that the researcher wants delivered to specific target cells. This genome ... See article for more cre-lox shRNA expression variants. Jacks 19319 pLJM1-EGFP 3rd CMV-driven EGFP fusion... 11619 pLB 3rd Modification of pLL3.7; Genetic elements known to prevent epigenetic silencing were added...

  18. Tetracycline Inducible Expression

    Type
    Collection
    ...tetracycline-regulated gene expression and find plasmids for your experiments using tet- or dox-inducible systems....system has been engineered with a variety of improvements. A synthetic Tet Response Element (TRE) typically...been designed by combining tet O sequences with elements like the H1 RNA promoter. And, most importantly...Tet-Off and Tet-On. Read on to learn more about the components of tet systems and how to use them, or skip ahead...activation domain). For simplicity, other promoter elements are not shown. Tetracycline Off (Tet-Off) The ... Systems Subsequent efforts have improved the components of these systems for better efficiency, sensitivity...). rtTA3 and Tet-On 3G : further improved rtTA variants with lower background activity and greater sensitivity...

  19. Distribution to Industry

    Type
    Collection
    ...to be diverse in topology, size, and isoelectric points. Pooled Libraries Pooled Library name Type PI Description...payment, and billing. Industry Material Transfer Agreements Most Addgene orders must be accompanied by a ...protection for material providers. Addgene's deposit agreements allow Addgene to distribute plasmids on behalf...behalf of the depositing institutions. These agreements indicate the language which we are allowed to use...use for distribution. Please see the Sample Agreements on our Technology Transfer Information page for ...authorized signatory is someone who can execute legal documents on behalf of the recipient institution. Once the...addgene.org . After we review and approve the MTA documents, your order will be processed and shipped. Industry...

  20. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Empty backbones are plasmids containing only the elements needed for replication in the host cell and are...choosing what plasmid backbone to use, you have many elements to consider. Here is a guide to a selection of... 3x HA tag fusion with your gene of interest in plants Myc Epitope tag pKMyc - N-terminal Myc tag for ...-nosT - Transient expression vector for eGFP in plants Find FP tagging vectors in budding yeast pHT101... transient expression is sufficient for some experiments, scientists often want to create stable ...species Vectors for RNA-guided genome editing in plants Visit our CRISPR Plasmids and Resources collection... miRNA and 3' UTR mRNA regulation pIS0 - 3'UTR segments of target genes can be inserted into this firefly...
Showing: 1 - 20 of 114 results