We narrowed to 741 results for: REV
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TypeBlog Post...doi.org/10.1038/nmeth.3871 Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib...
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Meet an Addgenie: Insights into Grad School, Career Paths, and More
TypeBlog Post...when I was introduced to bioinformatics in the previous programs. I thought, ‘Let me pursue this, let ... -
Plasmids 101: Modular Cloning
TypeBlog Post...surprised by how multi-purpose it can be. Let’s review a list of MoClo applications. Genetic circuits ... -
Progress Towards a PAM-Free CRISPR
TypeBlog Post...and PAM-flexible nucleases! In this blog, we will review Cas enzymes, which have a flexible or non-existent... -
Hot Plasmids February 2024
TypeBlog Post...(NHP) brains. Systemic gene delivery capsids previously identified in rodents have not been widely successful... -
Viral Vectors 101: Calcium Sensors
TypeBlog Post...nucleus-targeted GCaMP6 with an extra apoCaM-binding motif to prevent aberrant binding of the biosensor to endogenous... -
Viral Vectors 101: Chemogenetics
TypeBlog Post.../364/6436/eaav5282.long Redfern CH, Coward P, Degtyarev MY, Lee EK, Kwa AT, Hennighausen L, Bujard H, ... -
Antibodies 101: Choosing the Right Isotype
TypeBlog Post...choice affect my experiment? In this post we will review some of the main factors that affect isotype choice... -
Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)
TypeBlog Post...Boguszewska, K., Szewczuk, M., Urbaniak, S. et al. Review: immunoassays in DNA damage and instability detection... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog Post...issues.” We then ask the depositing laboratory to review the discrepancies. If the depositing laboratory... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog Post...editing with a single Cas9-VPR in Saccharomyces cerevisiae. To control Cas9 activity and influence DNA repair... -
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells
TypeBlog Post...expression plasmids. These results are in line with previous research showing that the method of Cas9 and gRNA... -
Engaging with science and society at pgEd
TypeBlog Post...benefiting from the fruits of scientific research, and prevent technological advances from exacerbating existing... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog Post...investigations of the mammalian genome that can reveal how genetic alterations lead to changes in phenotype... -
Split-BioID: An Improved Method for Studying Protein-Protein Interactions
TypeBlog Post...pulldown step, such as AP-MS. This requirement often prevents the identification of weak and/or transient interacting... -
Lab Automation at Addgene
TypeBlog Post...decided on Beckman Coulter’s Biomek FXP. We had a previous relationship with Beckman Coulter and that certainly... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog Post...proteins available. Fortunately, several excellent reviews have collated data from various papers to make ... -
Plasmids for Endogenous Gene Tagging in Human Cells
TypeBlog Post...found within the homology areas of the plasmid to prevent the plasmid from being cut and destroyed by CRISPR... -
Tips for Technical Support Calls
TypeBlog Post... libraries were originally described in a peer-reviewed scientific publication. We love science, but we... -
Adapting Toehold Switches to Detect Zika Virus
TypeBlog Post...Synthetic Biology Posts Listen to Our Interview with Previous Collin's Lab Member and Sample 6 Cofounder Michael...