We narrowed to 741 results for: REV

1. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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   ...doi.org/10.1038/nmeth.3871 Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib...

2. Meet an Addgenie: Insights into Grad School, Career Paths, and More

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   ...when I was introduced to bioinformatics in the previous programs. I thought, ‘Let me pursue this, let ...

3. Plasmids 101: Modular Cloning

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   ...surprised by how multi-purpose it can be. Let’s review a list of MoClo applications. Genetic circuits ...

4. Progress Towards a PAM-Free CRISPR

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   ...and PAM-flexible nucleases! In this blog, we will review Cas enzymes, which have a flexible or non-existent...

5. Hot Plasmids February 2024

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   ...(NHP) brains.  Systemic gene delivery capsids previously identified in rodents have not been widely successful...

6. Viral Vectors 101: Calcium Sensors

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   ...nucleus-targeted GCaMP6 with an extra apoCaM-binding motif to prevent aberrant binding of the biosensor to endogenous...

7. Viral Vectors 101: Chemogenetics

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   .../364/6436/eaav5282.long Redfern CH, Coward P, Degtyarev MY, Lee EK, Kwa AT, Hennighausen L, Bujard H, ...

8. Antibodies 101: Choosing the Right Isotype

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   ...choice affect my experiment? In this post we will review some of the main factors that affect isotype choice...

9. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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   ...Boguszewska, K., Szewczuk, M., Urbaniak, S. et al. Review: immunoassays in DNA damage and instability detection...

10. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    ...issues.” We then ask the depositing laboratory to review the discrepancies. If the depositing laboratory...

11. Hot Plasmids and Viral Preps - March 2021

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    ...editing with a single Cas9-VPR in Saccharomyces cerevisiae. To control Cas9 activity and influence DNA repair...

12. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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    ...expression plasmids. These results are in line with previous research showing that the method of Cas9 and gRNA...

13. Engaging with science and society at pgEd

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    ...benefiting from the fruits of scientific research, and prevent technological advances from exacerbating existing...

14. Popular Retroviral Vectors and Their Uses in Scientific Research

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    ...investigations of the mammalian genome that can reveal how genetic alterations lead to changes in phenotype...

15. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

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    ...pulldown step, such as AP-MS. This requirement often prevents the identification of weak and/or transient interacting...

16. Lab Automation at Addgene

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    ...decided on Beckman Coulter’s Biomek FXP. We had a previous relationship with Beckman Coulter and that certainly...

17. Fluorescent Proteins 101: Introduction to FRET

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    ...proteins available. Fortunately, several excellent reviews have collated data from various papers to make ...

18. Plasmids for Endogenous Gene Tagging in Human Cells

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    ...found within the homology areas of the plasmid to prevent the plasmid from being cut and destroyed by CRISPR...

19. Tips for Technical Support Calls

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    ... libraries were originally described in a peer-reviewed scientific publication. We love science, but we...

20. Adapting Toehold Switches to Detect Zika Virus

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    ...Synthetic Biology Posts Listen to Our Interview with Previous Collin's Lab Member and Sample 6 Cofounder Michael...