We narrowed to 741 results for: REV

1. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

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   ...pulldown step, such as AP-MS. This requirement often prevents the identification of weak and/or transient interacting...

2. Lab Automation at Addgene

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   ...decided on Beckman Coulter’s Biomek FXP. We had a previous relationship with Beckman Coulter and that certainly...

3. Plasmids 101: The Wide World of Natural Plasmids

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   ...Potential Applications in Biotechnology. Critical Reviews in Biotechnology, 24(4), 155–208. https://doi.org...

4. Hot Plasmids - October 2020

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   ...adult-born granule cells over time that were not previously possible.   Bensussen S, et al., iScience 2020...

5. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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   ...doi.org/10.1038/nmeth.3871 Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib...

6. Meet an Addgenie: Insights into Grad School, Career Paths, and More

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   ...when I was introduced to bioinformatics in the previous programs. I thought, ‘Let me pursue this, let ...

7. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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   ...expression plasmids. These results are in line with previous research showing that the method of Cas9 and gRNA...

8. Fluorescent Proteins 101: Introduction to FRET

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   ...proteins available. Fortunately, several excellent reviews have collated data from various papers to make ...

9. Plasmids for Endogenous Gene Tagging in Human Cells

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   ...found within the homology areas of the plasmid to prevent the plasmid from being cut and destroyed by CRISPR...

10. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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    ...Boguszewska, K., Szewczuk, M., Urbaniak, S. et al. Review: immunoassays in DNA damage and instability detection...

11. Plasmids 101: Modular Cloning

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    ...surprised by how multi-purpose it can be. Let’s review a list of MoClo applications. Genetic circuits ...

12. Progress Towards a PAM-Free CRISPR

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    ...and PAM-flexible nucleases! In this blog, we will review Cas enzymes, which have a flexible or non-existent...

13. Hot Plasmids February 2024

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    ...(NHP) brains.  Systemic gene delivery capsids previously identified in rodents have not been widely successful...

14. Viral Vectors 101: Calcium Sensors

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    ...nucleus-targeted GCaMP6 with an extra apoCaM-binding motif to prevent aberrant binding of the biosensor to endogenous...

15. Viral Vectors 101: Chemogenetics

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    .../364/6436/eaav5282.long Redfern CH, Coward P, Degtyarev MY, Lee EK, Kwa AT, Hennighausen L, Bujard H, ...

16. Antibodies 101: Choosing the Right Isotype

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    ...choice affect my experiment? In this post we will review some of the main factors that affect isotype choice...

17. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    ...issues.” We then ask the depositing laboratory to review the discrepancies. If the depositing laboratory...

18. Hot Plasmids and Viral Preps - March 2021

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    ...editing with a single Cas9-VPR in Saccharomyces cerevisiae. To control Cas9 activity and influence DNA repair...

19. Tips for Technical Support Calls

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    ... libraries were originally described in a peer-reviewed scientific publication. We love science, but we...

20. Adapting Toehold Switches to Detect Zika Virus

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    ...Synthetic Biology Posts Listen to Our Interview with Previous Collin's Lab Member and Sample 6 Cofounder Michael...