We narrowed to 783 results for: SCR;

1. Antibodies 101: Beyond Surface Labeling

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   ...the same). Examples of such targets include transcription factors, histones, and DNA repair enzymes. Note...

2. An Integrin Antibody Toolkit from IPI

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   ...collection includes 26 unique antibodies that bind discrete subunits or particular subunit pairs. Some of ...

3. New Optogenetic Tools for Cytoskeleton and Membrane Control

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   ...plasma membrane. In previous publications, they described tools to control formation of lamellipodia and...

4. Tips for Getting a Faculty Position

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   ...feedback. Many of these documents are too vaguely described in other sources of guidance or the document examples...

5. Fluorescent Proteins 101: When GFP lets you down

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   ...probes are based on cofactor binding proteins as described above. The smallest tags are peptides that are...

6. 10 Steps to a Perfect Science Talk

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   ...listeners to be thinking “What happens next?”  Describe how you pushed to a solution or overcame obstacles...

7. Thoughts on the Future of Research 2015 Boston Symposium

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   ...PI on the panel even suggested projecting your manuscript onto a wall and reading it out loud. While it...

8. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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   ... capsid of another. Formally, pseudotypes are described with two numbers separated by a slash: the first...

9. CRISPR 101: Off-Target Effects

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   ...differences. If you are performing CRISPR library screens, the same principle holds true - the higher the...

10. Viral Vectors 101: Optogenetic Tools

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    ...In 2005, Boyden et al. described the first light-activated tool for controlling neuronal activity, channelrhodopsin...

11. Antibodies 101: Multiplex Immunofluorescence

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    ...between the targets. Or, it might be sufficient to describe or classify the labeled structures visually; for...

12. Generating Mouse Models Using CRISPR/Cas9

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    ...selection offered by mouse ESCs. For example, we often screen only 15-25 mice when generating knockout models...

13. Transferable Skills Guide: Teamwork

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    ...members could be recruited, but certainly not conscripted. There’s also no end-game here, unless we manage...

14. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

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    ...fluorescent donors and acceptors in their product descriptions, or technical manuals. Specific applications...

15. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

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    ...transdifferentiation is usually carried out by expressing transcription factors specific to the lineage of the target...

16. A Needle in a Base-Stack: Cas9 Structural Biology

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    ...Fact! Even though the Cas9:RNA complex is well described in the closed conformation, recent electron microscopy...

17. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

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    ...institution: This criterion is consistently under scrutiny. USCIS wants you to prove that without your critical...

18. CRISPR 101: Cytosine and Adenine Base Editors

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    ...named ABEmax. In 2020, two papers were published describing additional ABEs evolved from ABE7.10 that have...

19. Technical Design of a Western Blot

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    Blog Post

    ...using previously published antibodies, check the manuscript and supplementary figures for validation data...

20. CRISPR Guide

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    ...integration of donor DNA. Genome-Wide Screens Using CRISPR In a genetic screen, a population of cells with mutations...Targeting dCas9 to transcription start sites is sufficient to repress transcription by blocking initiation..., G. M. (2013). CAS9 transcriptional activators for target specificity screening and paired nickases for...in your lab, from knockouts to pooled library screening. Science...CRISPR Basics Small Edits Large Edits Genome-Wide Screening Cas9 Fusion Tools Cas9 Alternatives Resources ...approaches like zinc finger nucleases (ZFNs) or transcription-activator-like effector nucleases (TALENs) required...technologies, making CRISPR perfect for genome-wide screens. This guide will provide a basic understanding ...