We narrowed to 876 results for: lic
-
TypeBlog Post...instances, variations of the product’s specific application instructions or “optimized steps” can be linked...customers may tell all sorts of details about the application, just not the ones that matter. Therefore, if...
-
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program
TypeBlog Post...community is no small task. The EDGE program solicitation requires funded labs to rapidly disseminate ...dissemination of information and materials. Many groups publicized workshops on their organismal systems for scientists... -
Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names
TypeBlog Post...transposon system including those from the original publication, Ivics et al 1997. Spaghetti Squash, a regulator...bunch of cookie monster eyes.” Check out the publication to see for yourself. We have one plasmid in our... -
Lab to Office Culture Shock
TypeBlog Post...involves a few key features that can be difficult to replicate in other work settings: The sense of being the... scientist position at Addgene, the skeptical applicant asked if she would partake in “critical thinking... -
Supporting Reproducibility with a Connected ELN
TypeBlog Post... vehicle for making research data available for public access, review, and query. Gates Open Research ... both accept deposit of data associated with publications into one of five general purpose open source... -
New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression
TypeBlog Post... the bacteria were injected, limiting their applicability. They also did not scatter ultrasound nonlinearly...promoter pBAD and a low-copy number origin of replication (p15A) worked best under a wide range of inducer... -
Bioinformatics at Addgene
TypeBlog Post... from depositing laboratories and associated publications, but our quality control (QC) standards have...can be easily shared with others without any complicated setup. We created, tested, and debugged each ... -
Hot Plasmids - August 2020
TypeBlog Post...protein evolution By Joanne Kamens Mateo Sanchez and Alice Ting have used one biotechnology tool to optimize...cultured and FACS sorted. Image from Mateo Sanchez and Alice Ting. Sanchez MI et al., Nat methods. 2019. https... -
Replacing Paper: Tips for Choosing an Electronic lab Notebook
TypeBlog Post...priorities in most cases include IP protection, publications and funding. If any of these three pillars crashes...working for a large institution or company, site licenses might be available as well. In most cases this... -
Multiple Plasmids at a Low Price: Kits from Addgene
TypeBlog Post...polycistronic E. coli expression constructs. Some applications of plasmids from this kit include the production...variety of functions via different fusions. Some applications of PUF protein fusions reported in the literature... -
Transferable Skills Guide: Time Management
TypeBlog Post... progress. For this, I always make sure I have explicit goals in mind for the endpoints of a task or project...instance, if I don’t have draft blog post ready for publication for tomorrow, I’d better upload that post from... -
Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping
TypeBlog Post...proteins A year after BioID became available, the Alice Ting Lab demonstrated that engineered ascorbate ... consists of a humanized biotin ligase from A. aeolicus, which lacks the DNA-binding domain (233 amino... -
How to Negotiate a Successful Lab Start Up Budget
TypeBlog Post... most start-up P.I.’s. It doesn’t have to be complicated, or difficult to create this list. Start BEFORE...discounts, collaborations, access to internal award applications, etc. If this is the institute that can help... -
Plasmids 101: Common Lab E. coli Strains
TypeBlog Post...that carried out the labor-intensive process of replicating your new plasmid: the Escherichia coli bacteria... Most Popular Plasmids 101 Posts: Origin of Replication The Promoter Region – Let's Go! Mammalian Vectors... -
Technique: Probe Phage Genomes for Host Binding Proteins
TypeBlog Post... DNA from host cell nucleases (4). To further complicate matters, even when sequenced, phage genomes tend...Franklin L., et al. "Revisiting phage therapy: new applications for old resources." Trends in microbiology 23.4... -
Easi-CRISPR: Generating Knock-In and Conditional Mouse Models
TypeBlog Post...previously made mouse model. To avoid disrupting splice donor/acceptor sites, Miura et al. (2018) recommend...Used with permission under a Creative Commons 4.0 license. The research groups responsible for Easi-CRISPR... -
Technologies Enabled by NanoLuc® Luciferase
TypeBlog Post...et al (2). In this post, I’ll cover two great applications of the NLuc technology. The first couples NLuc...fluorescence. The paper reports a wide variety of applications of the Enhanced Nano-Lanterns including monitoring... -
CRISPR 101: Off-Target Effects
TypeBlog Post...sequencing. While this may be unnecessary for many applications, it is the only comprehensive method available...you’ve tried everything listed here. For some applications, this is ok, but it still must be controlled... -
Finding Your Perfect Job After University
TypeBlog Post...recession. As each advertised job received many applications, employers were requesting that only scientists...from other individuals projects, this can cause conflicts and favoritism, as well as affect morale and cause... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog Post...but, in numerous studies, optogenetics is used in slice physiology in order to directly show that, for example...22430017. 2. Fenno L, et al. The development and application of optogenetics. Annual review of neuroscience...