We narrowed to 884 results for: lic

...PIPs, resulting in a fluorescent readout.   Applications for bimolecular fluorescence complementation...resulting from the assayed PPI. This high-throughput application is much more straightforward to carry out because...

...missing so that we can make a new tube from a replicate sample. The transformation After the DNA has ...plasmid can grow. As the bacteria divide, they replicate and retain the plasmid. This allows us to make...

...instances, variations of the product’s specific application instructions or “optimized steps” can be linked...customers may tell all sorts of details about the application, just not the ones that matter. Therefore, if...

...submitted to Addgene have minor differences due to in silico variations and lack of verification. To address...Nanopore sequencing technology, bioinformatics and applications. Nature Biotechnology, 39(11), 1348–1365. https...

...community is no small task. The EDGE program solicitation requires funded labs to rapidly disseminate ...dissemination of information and materials. Many groups publicized workshops on their organismal systems for scientists...

...transposon system including those from the original publication, Ivics et al 1997. Spaghetti Squash, a regulator...bunch of cookie monster eyes.”  Check out the publication to see for yourself. We have one plasmid in our...

...involves a few key features that can be difficult to replicate in other work settings: The sense of being the... scientist position at Addgene, the skeptical applicant asked if she would partake in “critical thinking...

...protein evolution By Joanne Kamens Mateo Sanchez and Alice Ting have used one biotechnology tool to optimize...cultured and FACS sorted. Image from Mateo Sanchez and Alice Ting. Sanchez MI et al., Nat methods. 2019. https...

... the bacteria were injected, limiting their applicability. They also did not scatter ultrasound nonlinearly...promoter pBAD and a low-copy number origin of replication (p15A) worked best under a wide range of inducer...

... from depositing laboratories and associated publications, but our quality control (QC) standards have...can be easily shared with others without any complicated setup. We created, tested, and debugged each ...

... vehicle for making research data available for public access, review, and query. Gates Open Research ... both accept deposit of data associated with publications into one of five general purpose open source...

...be part of the answer. Problems with written publications Online tools like ResearchGate, GoogleScholar...Freedom: The Expansion of Data Sharing in Research Publications Resources at Addgene.org Check out our Protocol...

... progress. For this, I always make sure I have explicit goals in mind for the endpoints of a task or project...instance, if I don’t have draft blog post ready for publication for tomorrow, I’d better upload that post from...

...priorities in most cases include IP protection, publications and funding. If any of these three pillars crashes...working for a large institution or company, site licenses might be available as well. In most cases this...

...proteins A year after BioID became available, the Alice Ting Lab demonstrated that engineered ascorbate ... consists of a humanized biotin ligase from A. aeolicus, which lacks the DNA-binding domain (233 amino...

...polycistronic E. coli expression constructs. Some applications of plasmids from this kit include the production...variety of functions via different fusions. Some applications of PUF protein fusions reported in the literature...

... most start-up P.I.’s. It doesn’t have to be complicated, or difficult to create this list. Start BEFORE...discounts, collaborations, access to internal award applications, etc. If this is the institute that can help...

...that carried out the labor-intensive process of replicating your new plasmid: the Escherichia coli bacteria... Most Popular Plasmids 101 Posts: Origin of Replication The Promoter Region – Let's Go! Mammalian Vectors...

...previously made mouse model. To avoid disrupting splice donor/acceptor sites, Miura et al. (2018) recommend...Used with permission under a Creative Commons 4.0 license. The research groups responsible for Easi-CRISPR...

...sequencing. While this may be unnecessary for many applications, it is the only comprehensive method available...you’ve tried everything listed here. For some applications, this is ok, but it still must be controlled...