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Showing: 621 - 640 of 871 results
  1. Twenty Years of Addgene Sharing: CRISPR

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    Blog Post
    ... into Addgene’s CRISPR data? Read our recent publication, “The Expanding Dissemination and Distribution...support for, this and previous Addgene CRISPR publications.  References and resources References Pyhtila...
  2. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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    Blog Post
    ...targeting anywhere in an exon. But things get more complicated if you’d like to use homology directed repair...editing rates. This PAM flexibility should expand applications that require very specific targeting, like homology...
  3. 3D Printing Meets CRISPR Cas9

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    Blog Post
    ...moments. The models function as thinking tools – eliciting questions like “what does this model representt...or trying to explain your science to the general public, visit the Center for BioMolecular Modeling web...
  4. Tips for Using BLAST to Verify Plasmids

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    Blog Post
    ..., blastp, and blastx searches! Now, before you click the BLAST button, consider the Program Selection...may have noticed the “Algorithm parameters” link. Click on this link to view advanced BLAST options and ...
  5. AAVs in Retinal Gene Therapy

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    Blog Post
    ...muscular atrophy, neurodegenerative diseases and metabolic diseases are also underway and reported results...-1250015778 7. Yu W and Wu Z., 2018. In vivo Applications of CRISPR-Based Genome Editing in the Retina...
  6. Course-Based Undergraduate Research Experiences (CUREs)

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    Blog Post
    ...are often associated with rote memorization of metabolic pathways (precisely the type of exercise Einstein...mitochondrial MDH (hMDH2) with those from human cytosolic MDH (hMDH1). They then expressed the original ...
  7. Career Insights: Technical Support Specialist

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    Blog Post
    ...instances, variations of the product’s specific application instructions or “optimized steps” can be linked...customers may tell all sorts of details about the application, just not the ones that matter. Therefore, if...
  8. Hot Plasmids - August 2020

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    Blog Post
    ...protein evolution By Joanne Kamens Mateo Sanchez and Alice Ting have used one biotechnology tool to optimize...cultured and FACS sorted. Image from Mateo Sanchez and Alice Ting. Sanchez MI et al., Nat methods. 2019. https...
  9. Lab to Office Culture Shock

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    Blog Post
    ...involves a few key features that can be difficult to replicate in other work settings: The sense of being the... scientist position at Addgene, the skeptical applicant asked if she would partake in “critical thinking...
  10. Supporting Reproducibility with a Connected ELN

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    Blog Post
    ... vehicle for making research data available for public access, review, and query. Gates Open Research ... both accept deposit of data associated with publications into one of five general purpose open source...
  11. QC Sequencing Technologies at Addgene

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    Blog Post
    ...submitted to Addgene have minor differences due to in silico variations and lack of verification. To address...Nanopore sequencing technology, bioinformatics and applications. Nature Biotechnology, 39(11), 1348–1365. https...
  12. Bioinformatics at Addgene

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    Blog Post
    ... from depositing laboratories and associated publications, but our quality control (QC) standards have...can be easily shared with others without any complicated setup. We created, tested, and debugged each ...
  13. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...be part of the answer. Problems with written publications Online tools like ResearchGate, GoogleScholar...Freedom: The Expansion of Data Sharing in Research Publications Resources at Addgene.org Check out our Protocol...
Showing: 621 - 640 of 871 results