We narrowed to 862 results for: lic
-
TypeBlog Post...moments. The models function as thinking tools – eliciting questions like “what does this model representt...or trying to explain your science to the general public, visit the Center for BioMolecular Modeling web...
-
Cloning Mammalian Cells with the Agarose Method
TypeBlog Post..., and making sure we always maintain a backup replicates of each clone in case of contamination. We do...population of transfected clones. The technique is applicable to a wide variety of cell lines, from HEK to ... -
Tips for Using BLAST to Verify Plasmids
TypeBlog Post..., blastp, and blastx searches! Now, before you click the BLAST button, consider the Program Selection...may have noticed the “Algorithm parameters” link. Click on this link to view advanced BLAST options and ... -
Course-Based Undergraduate Research Experiences (CUREs)
TypeBlog Post...are often associated with rote memorization of metabolic pathways (precisely the type of exercise Einstein...mitochondrial MDH (hMDH2) with those from human cytosolic MDH (hMDH1). They then expressed the original ... -
AAVs in Retinal Gene Therapy
TypeBlog Post...muscular atrophy, neurodegenerative diseases and metabolic diseases are also underway and reported results...-1250015778 7. Yu W and Wu Z., 2018. In vivo Applications of CRISPR-Based Genome Editing in the Retina... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog Post...PIPs, resulting in a fluorescent readout. Applications for bimolecular fluorescence complementation...resulting from the assayed PPI. This high-throughput application is much more straightforward to carry out because... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog Post...missing so that we can make a new tube from a replicate sample. The transformation After the DNA has ...plasmid can grow. As the bacteria divide, they replicate and retain the plasmid. This allows us to make... -
New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression
TypeBlog Post... the bacteria were injected, limiting their applicability. They also did not scatter ultrasound nonlinearly...promoter pBAD and a low-copy number origin of replication (p15A) worked best under a wide range of inducer... -
Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names
TypeBlog Post...transposon system including those from the original publication, Ivics et al 1997. Spaghetti Squash, a regulator...bunch of cookie monster eyes.” Check out the publication to see for yourself. We have one plasmid in our... -
Lab to Office Culture Shock
TypeBlog Post...involves a few key features that can be difficult to replicate in other work settings: The sense of being the... scientist position at Addgene, the skeptical applicant asked if she would partake in “critical thinking... -
Supporting Reproducibility with a Connected ELN
TypeBlog Post... vehicle for making research data available for public access, review, and query. Gates Open Research ... both accept deposit of data associated with publications into one of five general purpose open source... -
Career Insights: Technical Support Specialist
TypeBlog Post...instances, variations of the product’s specific application instructions or “optimized steps” can be linked...customers may tell all sorts of details about the application, just not the ones that matter. Therefore, if... -
Bioinformatics at Addgene
TypeBlog Post... from depositing laboratories and associated publications, but our quality control (QC) standards have...can be easily shared with others without any complicated setup. We created, tested, and debugged each ... -
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program
TypeBlog Post...community is no small task. The EDGE program solicitation requires funded labs to rapidly disseminate ...dissemination of information and materials. Many groups publicized workshops on their organismal systems for scientists... -
Hot Plasmids - August 2020
TypeBlog Post...protein evolution By Joanne Kamens Mateo Sanchez and Alice Ting have used one biotechnology tool to optimize...cultured and FACS sorted. Image from Mateo Sanchez and Alice Ting. Sanchez MI et al., Nat methods. 2019. https... -
Using Video to Share Your Science: We Share Science
TypeBlog Post...be part of the answer. Problems with written publications Online tools like ResearchGate, GoogleScholar...Freedom: The Expansion of Data Sharing in Research Publications Resources at Addgene.org Check out our Protocol... -
Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping
TypeBlog Post...proteins A year after BioID became available, the Alice Ting Lab demonstrated that engineered ascorbate ... consists of a humanized biotin ligase from A. aeolicus, which lacks the DNA-binding domain (233 amino... -
Replacing Paper: Tips for Choosing an Electronic lab Notebook
TypeBlog Post...priorities in most cases include IP protection, publications and funding. If any of these three pillars crashes...working for a large institution or company, site licenses might be available as well. In most cases this... -
Transferable Skills Guide: Time Management
TypeBlog Post... progress. For this, I always make sure I have explicit goals in mind for the endpoints of a task or project...instance, if I don’t have draft blog post ready for publication for tomorrow, I’d better upload that post from... -
Multiple Plasmids at a Low Price: Kits from Addgene
TypeBlog Post...polycistronic E. coli expression constructs. Some applications of plasmids from this kit include the production...variety of functions via different fusions. Some applications of PUF protein fusions reported in the literature...