We narrowed to 760 results for: mpo
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TypeBlog Post...proteins, and membrane proteins. Although all viral components are available in bacterial expression plasmids...
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Hot Plasmids - May 2022
TypeBlog Post...expression in the liver after systemic delivery. Importantly, this is true in both mice and nonhuman primates... -
Hot Plasmids - February 2022
TypeBlog Post...CRISPR-Cas system for editing and gene activation, is composed of only 529 amino acids, less than half the size... -
From Lab Bench to Leadership: Transitioning into Management as a PhD
TypeBlog Post..., I recognized the need to shift my mindset to empower my team and allow myself to focus on my core responsibilities... -
Clear and Accessible Writing
TypeBlog Post..., and we’d love to hear yours as well. More importantly, you can use these four steps for any type of... -
CasPEDIA: A Functional Classification of Cas Enzymes
TypeBlog Post...how can I multiplex this enzyme? What other RNA components are required? CasPEDIA provides information on... -
Multiplex Genome Editing with CRISPR-Cpf1
TypeBlog Post...Cas9 and Cpf1 CRISPR Nucleases Cas9 Cpf1 Components crRNAtracrRNACas9 crRNACpf1 Endonuclease... -
Antibodies 101: Flow Cytometry Controls
TypeBlog Post...point for compensation (Fig. 1B-C). However, it’s important to note that because you’re using beads and not... -
A Novice's Perspective on Neuroscience - SfN2017
TypeBlog Post...FRET-based biosensing technique can be applied to an important neuronal signalling pathway - if you’ve got a ... -
GPCRs: How Do They Work and How Do We Study Them?
TypeBlog Post... cell membrane. Heterotrimeric G proteins are important molecular switches in signal transduction pathways... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog Post... Zhang lab has shared over 40,000 CRISPR-Cas9 components over the years on their own or through Addgene... -
Early Career Researcher Toolbox: Social Media for Scientists
TypeBlog Post...can fit into a researcher’s work day. It’s also important to remember that it’s not the amount of time you... -
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools
TypeBlog Post...enzyme and a small-molecule substrate to map the spatiotemporal distribution and interaction networks of specific... -
Screening for Successful Genome Editing with Digital PCR
TypeBlog Post...nuclease assays are completely “blind” to this very important difference (Figure 3). If you are interested... -
Plasmids 101: Methylation and Restriction Enzymes
TypeBlog Post...protect themselves from foreign DNA. The other component of these systems, methyltransferases, methylate... -
How to Keep a Lab Notebook for Bioinformatic Analyses
TypeBlog Post...your methods section about the edit that was temporarily used. Just be sure that the original code remains... -
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers
TypeBlog Post...versions of this out there. Addgene: Why was it important to you to make these plasmids available to other... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog Post... of endogenous genes and it consists of three components: 1. A nucleolytically inactive Cas9-VP64 fusion... -
Will You Be My Mentor? Finding and Asking for Mentoring Support
TypeBlog Post...mentors from your current organization but also important to build relationships outside your organization... -
Seven Tips for Using LinkedIn as a Scientist
TypeBlog Post...had a chance to proof read. This might not be important for a good friend, but if you are making a professional...