We narrowed to 760 results for: mpo

1. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

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   ...enzyme and a small-molecule substrate to map the spatiotemporal distribution and interaction networks of specific...

2. New and Upcoming Viral Vectors - May 2020

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   .... The advantages of optogenetics lie in the spatiotemporal control offered by the system that allows for...

3. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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   ...proteins, and membrane proteins. Although all viral components are available in bacterial expression plasmids...

4. Antibodies 101: Flow Cytometry Controls

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   ...point for compensation (Fig. 1B-C). However, it’s important to note that because you’re using beads and not...

5. From Lab Bench to Leadership: Transitioning into Management as a PhD

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   ..., I recognized the need to shift my mindset to empower my team and allow myself to focus on my core responsibilities...

6. GPCRs: How Do They Work and How Do We Study Them?

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   ... cell membrane. Heterotrimeric G proteins are important molecular switches in signal transduction pathways...

7. 7 Tips to Secure a STEAM Internship This Summer

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   ... and lighting for a smooth performance. Most importantly, make certain that everyone you live with is ...

8. Hot Plasmids - May 2022

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   ...expression in the liver after systemic delivery.  Importantly, this is true in both mice and nonhuman primates...

9. Hot Plasmids - February 2022

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   ...CRISPR-Cas system for editing and gene activation, is composed of only 529 amino acids, less than half the size...

10. Clear and Accessible Writing

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    ..., and we’d love to hear yours as well.  More importantly, you can use these four steps for any type of...

11. CasPEDIA: A Functional Classification of Cas Enzymes

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    ...how can I multiplex this enzyme? What other RNA components are required? CasPEDIA provides information on...

12. A Novice's Perspective on Neuroscience - SfN2017

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    ...FRET-based biosensing technique can be applied to an important neuronal signalling pathway - if you’ve got a ...

13. Multiplex Genome Editing with CRISPR-Cpf1

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    ...Cas9 and Cpf1 CRISPR Nucleases   Cas9 Cpf1 Components crRNAtracrRNACas9 crRNACpf1 Endonuclease...

14. Screening for Successful Genome Editing with Digital PCR

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    ...nuclease assays are completely “blind” to this very important difference (Figure 3). If you are interested...

15. Plasmids 101: Methylation and Restriction Enzymes

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    ...protect themselves from foreign DNA. The other component of these systems, methyltransferases, methylate...

16. How to Keep a Lab Notebook for Bioinformatic Analyses

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    ...your methods section about the edit that was temporarily used. Just be sure that the original code remains...

17. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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    ...versions of this out there. Addgene: Why was it important to you to make these plasmids available to other...

18. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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    ... of endogenous genes and it consists of three components: 1. A nucleolytically inactive Cas9-VP64 fusion...

19. Will You Be My Mentor? Finding and Asking for Mentoring Support

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    ...mentors from your current organization but also important to build relationships outside your organization...

20. Transferable Skills Guide: Public Speaking

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    ...Averaging ten panels each. (For this story, it is important to know that the student presenting right before...