We narrowed to 904 results for: nes

1. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

   Type

   Blog Post

   ...was then screened through successive rounds of mutagenesis for the brightest teal fluorescent proteins that...scientists from Florida State University, they used a mutagenesis approach and found that specific amino acid changes...

2. New and Upcoming Viral Vectors - Spring 2019

   Type

   Blog Post

   ...with different properties (for example, overall brightness or on/off kinetics) that makes them suitable ...pAAV.hSyn-FLEX.iGABASnFR) has good membrane localization and brightness. The F102G variant (pAAV.hSyn-FLEX.iGABASnFR....

3. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

   Type

   Blog Post

   ... and genomic DNA in a variety of mammalian cell lines, with an efficiency similar to that of Cas9. Like...certain guide sequences - using 5 guides/gene for 10 genes, they did not observe differences in cleavage efficiency...

4. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

   Type

   Blog Post

   ...patients suffering from Parkinson's, chronic pain, deafness, or other conditions. Addgene: You've contributed...require less power. Those are two of the biggest ones. And as a third example, it would be helpful to ...

5. Predicting Adverse Reactions to Monoclonal Antibody Drugs

   Type

   Blog Post

   ...performed using traditional 2D assays with immune cell lines or peripheral blood mononuclear cells (PBMC) in .... P., Herskovitz, J., Goletz, T. J., Zhou, L., Moxness, M., Flynn, G. C., Narhi, L. O., & Jawa, V. (2016...

6. Trading Spaces - Visiting Scientist Edition

   Type

   Blog Post

   ... know that science usually doesn’t follow our timelines! Prepare a research plan to set yourself up for...applying to a structured program, they likely have guidelines to consult on this topic.   Etiquette...

7. K. phaffii: Rising to the Occasion

   Type

   Blog Post

   ...experimental model. In fact, approximately 25% of yeast genes have a human counterpart, enabling study of human-relevant...the strain tractable for peroxisomal studies on biogenesis, degradation, and proliferation. Genome manipulation...

8. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

   Type

   Blog Post

   ... power of collaboration, which despite the competitiveness of cutting-edge research, is still one of the...that research is done better with others than by oneself that prompted us to create Crowdfight.   ...

9. Networking Follow-up: I Met a Person. Now What?

   Type

   Blog Post

   ...my guess is that people will not be exchanging business cards for awhile or ever so your LinkedIn profile...She supported me and answered my questions and honestly, she was the only person who really understood...

10. 25 Work From Home Tips for Scientists

    Type

    Blog Post

    ... help you prepare for sleep. At Addgene, our mindfulness group is sticking with their Headspace practice...19. You can also give back by supporting local businesses (ex: purchasing a gift card to use later or ordering...

11. Cultivating Community Science at BosLab

    Type

    Blog Post

    ... with audiovisual media, software engineering, business consulting, mathematics, and more. That’s right...BosLab. Once he optimizes plant cell culture and transgenesis he wants to share his knowledge with the community...

12. Visualizing Genomic Loci with CRISPR-Sirius

    Type

    Blog Post

    ...the gRNA target site. In addition to increased brightness, they also found that the signal to noise ratio... observing low-copy genomic loci or single copy genes. If you want to visualize genomic loci brightly,...

13. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type

    Blog Post

    ...different types of tubes: the ones we use to store lentivirus and the ones we use to store AAV. Our results...

14. What Good Citizenship Can Do for Reproducibility in Science

    Type

    Blog Post

    ...going forward. This is not just a question of being honest and straightforward, it’s an important part of ... various different versions of checklists and guidelines to help authors understand what they are expected...

15. 5 Great Apps for Lab Life

    Type

    Blog Post

    ...smartphone to help me with my research in terms of preparedness, organization, and sharing data. Here are five...Stacey! Sean D. Stacey is a Ph.D. student at East Tennessee State University. He works with gene expression...

16. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type

    Blog Post

    ...disrupt target gene sequences (thus inactivating genes), while breaks repaired by homology directed repair... which sequence replacement holds some kind of fitness advantage but error-prone repair of the gene would...

17. Components of CRISPR/Cas9

    Type

    Blog Post

    ...Variant of Cas9 PAM Sequence Streptococcus pyogenes (SP); SpCas9 3' NGG SpCas9 D1135E variant...sites with the aid of two RNAs: the crRNA which defines the genomic target for Cas9, and the tracrRNA which...

18. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type

    Blog Post

    ... shift requires deliberate preparation and a willingness to embrace new responsibilities. After my second...between technical and managerial roles, and I've witnessed several colleagues do this while changing industries...

19. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type

    Blog Post

    ...the different populations. MFI is the average brightness from all cells that are positive for the marker...Prathima has a research background in protein mutagenesis and cancer immunotherapies. She is currently ...

20. GCE4All: Making Genetic Code Expansion Accessible

    Type

    Blog Post

    ...focus on development, along with the inherent trickiness of using and optimizing GCE, led to a large toolbox...essentially those needed to make site-directed mutagenesis, many researchers new to GCE aren’t able to successfully...