...their lab, in their experiments, to enable virtual learning during the pandemic. Feedback like that lets us... easier access to export-controlled items by streamlining permissions and paperwork. Through updating ...across the globe?” By asking this question, and by listening to the answers from our community, we’ll be able...

...Construction of solid matrix-antibody-antigen complexes containing simian immunodeficiency virus p27 using tag-specific... revealing antigenic differences between human, canine and simian isolates. J Gen Virol., 68 (Pt 11), ...transformed with Cas9 on an integrative plasmid containing tetracycline repressor TetR and resistance selection...

... Flow data is messy, so learning how to gate takes a lot longer than learning how to read a gating strategy...often easier to identify different populations by imagining (or drawing) a quadrant over the dot plot. Created...

...antibodies. The most famous ones are influenza hemagglutinin (HA), myelocytomatosis viral oncogene (myc),...Looger’s lab have developed new molecular tags combining the advantages of both (Viswanathan, 2015). For... in better resolution images. Conclusion By combining advantages of FPs and epitope tags, smFPs appear...

...activation experiments often require quite a bit of tuning before you know your system is working as expected...the best uses for Cas9 activators is in genetic screening. gRNAs targeting every gene in the human genome...Villalta JE, Chen Y, Whitehead EH, Guimaraes C, Panning B, Ploegh HL, Bassik MC, Qi LS, Kampmann M, Weissman...

...highly technical and this has a natural, albeit unintentional, tendency to work against transparency. This...will be in danger of having to deliver on well-meaning but impractical requirements imposed by publishers...Toronto, Canada where he studies protein-serine/threonine kinases implicated in human diseases. His spare...

...factors, therefore promoters play a large role in determining where and when your gene of interest will be ... UAS General expression mRNA Yeast promoter containing Gal4 binding sites, commonly used in Drosophila...increases expression of the repressor 10x, thus tightening regulation of lac promoter. Good for modulating...

...world manifesting as a full-fledged pandemic. Containing the spread of this virus has become an utmost...earlier epicenters of the outbreak ultimately flattening the curve, it has become evident that widespread...beads. Fluorescently labelled dCas9 complexes containing sgRNA that have no mismatches bind to the immobilised...

... E., & Gomes, A. V., “Stain-Free total protein staining is a superior loading control to β-actin for Western...more accurate as a loading control. Thanks for joining us in this exploration of western blot normalization... Gomes, A. V. (2013). Stain-Free total protein staining is a superior loading control to β-actin for Western...

...two vectors are part of the larger pMVP/pMAGIC cloning system, which contains Cas9 fusions for several...Arlotto ME, et al (2018) Creation of versatile cloning platforms for transgene expression and dCas9-based...CRISPR–Cas9 epigenome editing enables high-throughput screening for functional regulatory elements in the human...

...(1:24 - 29:31), there are a variety of ways of defining scientific reproducibility. For a study to be ...even simple procedures like pouring a plate or running a gel have intricacies that aren’t always mentioned...the biological sciences. I’d highly recommend listening to the panel discussion to learn a bit more about...

...”. One of my professors was excellent at phone cloning with labs around the world and had specific strategies...absorption by tissues. To avoid absorbance of heme-containing proteins in whole animals, light at or above ... is composed of Venus fluorescent protein, the Troponin C Ca++ binding domain, and NanoLuc® Luciferase...

...integrins3 Hepatocyte growth factor receptor (HGFR)3 Laminin receptor (LR)3 CD93 AAV5 2,3-N-linked sialic...growth factor receptor (PDGFR) α and β3 AAV8 Laminin receptor (LR)3 AAV receptor2   AAV9 Terminal...Terminal N-linked galactose3 AAV receptor2 Laminin receptor (LR)3 Putative integrin3 1Wu, Miller...

...2], [3]. Simply put, the piggyBac™ transposon containing a selection marker is included in a standard ...template to facilitate the selection of cells containing your desired edit. The selection is marker is...transposase enzyme [4]. By using a donor plasmid containing a negative selection marker such as thymidine...

...the second year in a row, agar art is one of the winning decorations for Deck The Lab. These festive, living...Kingdom. Kravitz Lab 2019 Holiday Door is up and running! @LexKravitz pic.twitter.com/J5bjHQo565 — Jess ...

...CRISPR, Addgene gave to me: tips and tricks for designing gRNAs for CRISPR editing. On the fourth day of...Addgene gave to me: anti-CRISPR proteins!  On the ninth day of CRISPR, Addgene gave to me: RNA editing with...

... to basic lab etiquette that will keep the lab running like a well-oiled machine. Lab tip #1: Glove trick...and press! Lab tip #2: Tube flick trick When combining reagents in the lab, it is important to mix them...

... us to ship rabies plasmids and viral vectors containing genetic elements from rabies plasmids to international...allows Addgene to ship plasmids or viral vectors containing sequences from rabies and all other lyssa genus...

...Hassabis and John Jumper! The Baker Lab focuses on designing entirely new proteins, improving our understanding...least that we’ve seen so far!). They started by designing a novel topology, then iteratively generated 3D...

...blogger Ava Shipman explains how the Wu lab is screening over 3,000 antibodies for use in mice and human...really incredible science - I hope you've enjoyed learning about it (and maybe even picked up a new technique...