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We narrowed to 958 results for: tes

Showing: 621 - 640 of 958 results

  1. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    ...year with a #DeckTheLab themed nativity scene, courtesy of our house pets.. šŸ˜ pic.twitter.com/qDylF8TdHH... social media throughout the year for more fun contests! Ā ...

  2. Plasmid Grant to Aid Cervical Cancer Screening in Ghana

    Type
    Blog Post
    ...screening in Ghana. While pap smears and visual tests are commonly used in Ghana, there is little screening... screening for HR HPV alongside the cytological tests currently in use. Gladys will use plasmids containing...

  3. Editor's Choice, October 2016

    Type
    Blog Post
    ...Building Community.ā€ This post leads readers to our latest podcast all about graduate student life and how...myself and two of my previous graduate school classmates, Ben Vincent and Niroshi Senaratne. When we recorded...

  6. Protocol - pLKO.1 ā€“ TRC Cloning Vector

    Type
    Protocol
    ...be worn at all times. Use plastic pipettes in place of glass pipettes or needles. Liquid waste should be...shRNA oligos are cloned into the AgeI and EcoRI sites in place of the stuffer. The AgeI site is destroyed... 10x NEB buffer 2 35 Ī¼L ddH 2 O Incubate for 4 minutes at 95Ā°C in a PCR machine or in a beaker of boiling...PCR machine, incubate the sample at 70Ā°C for 10 minutes then slowly cool to room temperature over the period...following manufacturerā€™s protocol. Plate on LB agar plates containing 100 Ī¼g/mL ampicillin or carbenicillin...swirling or gently flicking the tube. Incubate for 5 minutes at room temperature. e. Add 80 Ī¼L of FuGENEĀ® master...gently flicking the tube. f. Incubate for 20-30 minutes at room temperature. g. Retrieve HEK-293T cells...

  7. What's New in CRISPR - May 2019

    Type
    Blog Post
    ...demonstrate efficient editing in adult mice and in zygotes. Read the paper in Molecular Cell Find the Nme2Cas9... was developed by Rebecca Shapiroā€™s lab. After testing dCas9 fusions with transcriptional repressors, ...

  9. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    ...2015 was... lentiCRISPR V2 Ā  LentiCRISPR v2 originates from Feng Zhangā€™s lab at the Broad InstituteĀ ...secretion, and identifying lysine methyltransferase substrates. Weā€™re incredibly happy that lentiCRISPR v2 is...

  11. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    ...be repurposed for double strand breaks at target sites. Read the paper in Nature Communications Find...is a single step gRNA multiplexing system that generates vectors containing high numbers of gRNAs in one...

  15. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    ...transfers. The UBMTA was drafted by the National Institutes of Health (NIH) and adopted by the Association... Processing: When you place an order, Addgene generates the MTA and emails it straight to you, or we can...

  16. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    ...being plated on agar with ampicillin selection.Ā  Notes and tipsĀ  Weā€™ve found that our competent cells ...contamination.Ā  Also conduct a positive control (test a plasmid that transforms well in other competent...

  17. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...DNA for 2 minutes at 72Ā°C. Repeat steps 8-10 for 25-30 cycles. Final Extension for 5 minutes at 72Ā°C. ...oligo primers. This molecular biology technique creates several micrograms of target DNA from just a few.... Basic PCR Program Initial Denaturation for 2 minutes at 94Ā°C: This initiation step heats the double ...Repeat steps 2-4 25-30 times. Final Extension for 5 minutes at 72Ā°C: A final extension to fill-in any protruding...melting temperature (Tm). Set extension step at 1-2 minutes per kilobase of product depending on whether you...time and temperatures. Initial Denaturation for 2 minutes at 94Ā°C. Denature for 30 seconds at 94Ā°C. Anneal...denaturation of the DNA, particularly for GC-rich templates. What does each ingredient specifically do? Template...

  18. Editor's Choice, September 2016

    Type
    Blog Post
    ...Michael Koeris to learn how his company uses phage to test for Listeria in food products). I picked this post...Plasmids at a Low Price: Kits from Addgene Savvy Advocates Needed to Navigate a Scientific Enterprise in ...
Showing: 621 - 640 of 958 results