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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 955 results for: tes

Showing: 621 - 640 of 955 results

  2. What's New in CRISPR - May 2019

    Type
    Blog Post
    ...demonstrate efficient editing in adult mice and in zygotes. Read the paper in Molecular Cell Find the Nme2Cas9... was developed by Rebecca Shapiro’s lab. After testing dCas9 fusions with transcriptional repressors, ...

  3. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    ...2015 was... lentiCRISPR V2   LentiCRISPR v2 originates from Feng Zhang’s lab at the Broad Institute ...secretion, and identifying lysine methyltransferase substrates. We’re incredibly happy that lentiCRISPR v2 is...

  5. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    ...being plated on agar with ampicillin selection.  Notes and tips  We’ve found that our competent cells ...contamination.  Also conduct a positive control (test a plasmid that transforms well in other competent...

  6. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    ...be repurposed for double strand breaks at target sites. Read the paper in Nature Communications Find...is a single step gRNA multiplexing system that generates vectors containing high numbers of gRNAs in one...

  10. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    ...transfers. The UBMTA was drafted by the National Institutes of Health (NIH) and adopted by the Association... Processing: When you place an order, Addgene generates the MTA and emails it straight to you, or we can...

  11. Editor's Choice, September 2016

    Type
    Blog Post
    ...Michael Koeris to learn how his company uses phage to test for Listeria in food products). I picked this post...Plasmids at a Low Price: Kits from Addgene Savvy Advocates Needed to Navigate a Scientific Enterprise in ...

  14. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ...isolation of successful transformants. Addgene distributes several ready-to-use, modular plasmids, combining...combining fluorescent tags, epitope tags, protease sites, and selection markers. These are especially useful...

  15. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...be worn at all times. Use plastic pipettes in place of glass pipettes or needles. Liquid waste should be...shRNA oligos are cloned into the AgeI and EcoRI sites in place of the stuffer. The AgeI site is destroyed... 10x NEB buffer 2 35 μL ddH 2 O Incubate for 4 minutes at 95°C in a PCR machine or in a beaker of boiling...PCR machine, incubate the sample at 70°C for 10 minutes then slowly cool to room temperature over the period...following manufacturer’s protocol. Plate on LB agar plates containing 100 μg/mL ampicillin or carbenicillin...swirling or gently flicking the tube. Incubate for 5 minutes at room temperature. e. Add 80 μL of FuGENE® master...gently flicking the tube. f. Incubate for 20-30 minutes at room temperature. g. Retrieve HEK-293T cells...

  17. What's New in CRISPR - March 2020

    Type
    Blog Post
    ...pyogenes system does not work well in Caulobacter). To test their CRISPRi system, the Laub lab repressed ctrA...that the levels of ctrA mRNA decreased after 20 minutes in xylose (which controls expression of dCas9)....

  19. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ...gives a presentation on what they do and how it relates to other teams at the company. This provides new...old) employees with information on how Addgene operates as a team and shows how they fit into the system...

  20. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    ... to its modular structure. Two multiple cloning sites (MCS's) flank the M24 promoter, allowing it to be... customizing gene expression. Sahoo et al. have tested the system with genes up to 4 kb in length and ...
Showing: 621 - 640 of 955 results