New Podcast Segment: Hot Plasmids
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Blog Post
... some headphones to learn more about the latest and greatest plasmids through this podcast segment.
Below...
We narrowed to 964 results for: tes
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New Viral Vectors - Summer 2024
TypeBlog Post...repository Name Serotype Category Depositor Notes pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE...forget to use the link below to keep up with the latest in Addgene's viral vector services and resources... -
Addgene’s Newest Way to Speed Your Research: The Cloning Grade DNA Service
TypeBlog Post...Plasmids offered as cloning grade DNA As we begin testing out this service, we’re starting with 210 plasmids...next generation sequencing and spectrophotometry to test purity (260/280 nm and 260/230 nm ratios) and measure... -
New Viral Vectors - Spring 2025
TypeBlog Post...repository Name Serotype Category Depositor Notes pAAV-CAG-emiRFP670 AAV1 Control Kiryl Piatkevich...forget to use the link below to keep up with the latest in Addgene's viral vector services and resources... -
Congratulations, Deck The Lab winners!
TypeBlog Post...year with a #DeckTheLab themed nativity scene, courtesy of our house pets.. 😁 pic.twitter.com/qDylF8TdHH... social media throughout the year for more fun contests! ... -
Plasmid Grant to Aid Cervical Cancer Screening in Ghana
TypeBlog Post...screening in Ghana. While pap smears and visual tests are commonly used in Ghana, there is little screening... screening for HR HPV alongside the cytological tests currently in use. Gladys will use plasmids containing... -
Editor's Choice, October 2016
TypeBlog Post...Building Community.” This post leads readers to our latest podcast all about graduate student life and how...myself and two of my previous graduate school classmates, Ben Vincent and Niroshi Senaratne. When we recorded... -
Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding Labs
TypeBlog Post...as CRISPR-based gene repression tools to find and test genes that may play a role in the development of...polymerases produced using plasmids from Addgene to test for the presence of pathogens in environmental samples... -
Introducing an All-in-One CRISPR/Cas9 Vector System for Multiplex Genome Engineering
TypeBlog Post...Golden Gate cloning method to ligate expression cassettes of the gRNAs into a single vector in tandem. They...nickase vectors work successfully to target multiple sites in cultured human cells. While it has not yet been... -
What is Polymerase Chain Reaction (PCR)
TypeProtocol...DNA for 2 minutes at 72°C. Repeat steps 8-10 for 25-30 cycles. Final Extension for 5 minutes at 72°C. ...oligo primers. This molecular biology technique creates several micrograms of target DNA from just a few.... Basic PCR Program Initial Denaturation for 2 minutes at 94°C: This initiation step heats the double ...Repeat steps 2-4 25-30 times. Final Extension for 5 minutes at 72°C: A final extension to fill-in any protruding...melting temperature (Tm). Set extension step at 1-2 minutes per kilobase of product depending on whether you...time and temperatures. Initial Denaturation for 2 minutes at 94°C. Denature for 30 seconds at 94°C. Anneal...denaturation of the DNA, particularly for GC-rich templates. What does each ingredient specifically do? Template... -
Plasmid Preps: Different Purity, Different Quantities, Different Uses
TypeBlog Post...are used to insert recombinant DNA from prokaryotes or eukaryotes into a synthetic vector that can have ... -
What's New in CRISPR - May 2019
TypeBlog Post...demonstrate efficient editing in adult mice and in zygotes. Read the paper in Molecular Cell Find the Nme2Cas9... was developed by Rebecca Shapiro’s lab. After testing dCas9 fusions with transcriptional repressors, ... -
Your Top Requested Plasmid in 2015!
TypeBlog Post...2015 was... lentiCRISPR V2 LentiCRISPR v2 originates from Feng Zhang’s lab at the Broad Institute ...secretion, and identifying lysine methyltransferase substrates. We’re incredibly happy that lentiCRISPR v2 is... -
6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results
TypeBlog Post... This blog was originally published on BitesizeBio here. As part of my job ensuring plasmid quality at...Verify Plasmids Acknowledgements: Thank you BitesizeBio for originally publishing this and allowing us... -
Save Time and Money by Making Your Own Competent Cells
TypeBlog Post...being plated on agar with ampicillin selection. Notes and tips We’ve found that our competent cells ...contamination. Also conduct a positive control (test a plasmid that transforms well in other competent... -
Q&A With Julie Cicalese, the Newest Member of the Addgene Board of Directors
TypeBlog Post...compensation committee. The compensation committee creates a compensation philosophy that helps guide employee...spend time caring for my mother. For fun, I enjoy pilates, jewelry making, reading, sailing, and gardening... -
What's New in CRISPR - Spring 2019
TypeBlog Post...be repurposed for double strand breaks at target sites. Read the paper in Nature Communications Find...is a single step gRNA multiplexing system that generates vectors containing high numbers of gRNAs in one... -
CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping
TypeBlog Post...And could it be a new tool for genome editing? To test the potential of Cas14, the Doudna lab cloned the...of dsDNA and RNA in trans. Fluorescent signal indicates the presence of this viral sequence in the assay... -
Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton
TypeBlog Post...nature of the Center for Regenerative Medicine accelerates research. Chapter 5: Research currently under... defines Open Science and how he practices and promotes open science in the lab - sharing reagents, cell... -
Why Do I Need an MTA Anyway?
TypeBlog Post...transfers. The UBMTA was drafted by the National Institutes of Health (NIH) and adopted by the Association... Processing: When you place an order, Addgene generates the MTA and emails it straight to you, or we can...