We narrowed to 772 results for: LIS

1. Summer SciComm Series: Modes of Communication

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   Blog Post

   ...answered the above questions, you can create your short list of options and then pick whichever is most accessible...

2. Plasmids 101: Choosing an Antibiotic Resistance Gene

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   Blog Post

   ... toxic to dividing cells. The other antibiotics listed below affect continuous cellular processes (e.g...

3. DNA Purification Without a Kit

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   Blog Post

   ...adapted from the 1979 Patterson Protocol. When published, the Patterson Protocol was shown to purify DNA...

4. Droplet Digital PCR for AAV Quantitation

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   ... of parameters influencing droplet rain in the Listeria monocytogenes prfA assay-reduction of ambiguous...

5. Plasmids 101: Colony PCR

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   ...restriction enzyme digest; however colony PCR can accomplish the same thing in less time and for less money...

6. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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   ... the viral mRNAs and minigenome. This can be accomplished in multiple ways: transfecting with an additional...

7. Tips for Screening with Yeast Two Hybrid Systems

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   Blog Post

   ...to yeast transformation protocols also helped establish the yeast two-hybrid (Y2H) system as a robust ...

8. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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   Blog Post

   ...grant situation. So, young researchers need to publish good publications in a short period of time and...

9. Synthetic Biology - Browse Plasmids

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   Collection

   ...A list of Addgene plasmids for use in synthetic biology applications, or from synthetic biology labs....Standards SynBio Blog Genome Engineering Browse the list of available plasmids related to synthetic biology...are plasmids you would like to see added to this list, please email [email protected] . Synthetic Biology...

10. Plasmids 101: Codon usage bias

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    Blog Post

    ...consider when designing a full gene. A non-exhaustive list includes: Codon abundance relative to cognate tRNA...

11. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

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    ...safe-targeting guides as a control will result in a smaller list of true positive hits than when using non-targeting...

12. Neuronal labeling with Spaghetti Monster

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    Blog Post

    ...appear to be very effective probes for finely visualising sub-cellular structures or low-abundance proteins...

13. Analyzing CRISPR Editing Results with ICE from Synthego

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    Blog Post

    ... a graphical representation of the results and a list of the analyzed samples (see below).   If the sample...

14. Deciphering the Mysteries of Behavior with Viral Vectors

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    ...Central PMCID: PMC6333924. Howerton, Alexis R., Alison V. Roland, and Tracy L. Bale. "Dorsal raphe neuroinflammation...

15. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

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    ...within neuronal networks in living animals. To accomplish such cell specific manipulations in vivo, FLEx...

16. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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    Blog Post

    ...applications of CRISPR. Since this post was originally published, we've also seen the new development of Cas13b...

17. Twenty Years of Sharing: Addgene's Viral Vector Service

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    Blog Post

    ...work — in their system. The data is curated and published with a DOI, allowing others to reference and cite...

18. Hot Plasmids - August 2020

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    Blog Post

    ...mTurquoise2, mNeonGreen, and mScarlet-I for labeling a list of specific structures within cells. For example...

19. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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    ...have been used successfully in experiments and published in peer reviewed journals. If you need to generate...

20. Bioinformatics at Addgene

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    Blog Post

    ...Creating a bioinformatics workflow in Nextflow Establishing a complete pipeline however, required more than...