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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,010 results for: RING

Showing: 641 - 660 of 1010 results

  1. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ... the promiscuous cleavage and degradation of neighboring ssRNA and ssDNA by Cas13 and Cas12a, respectively....org/10.1038/s41587-020-0513-4 Chen JS, Ma E, Harrington LB, Da Costa M, Tian X, Palefsky JM, Doudna JA...Essletzbichler P, Dy AJ, Joung J, Verdine V, Donghia N, Daringer NM, Freije CA, Myhrvold C, Bhattacharyya RP, Livny...

  2. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...proteins (FP) have also been important in mapping, monitoring, and manipulating neuronal circuits. Epitope ...tags, low FP expression may be insufficient for monitoring a weakly expressed POI. Spaghetti Monster - A...fluorescent protein structure and color variants." Cold Spring Harbor Protocols 2009.12 (2009): pdb-top63. PubMed...

  3. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...first non-Cas9 CRISPR protein adapted for genome engineering was Cas12a (formerly Cpf1), a nuclease that generates...comment below. Note: Jennifer Tsang and Mary Gearing contributed to the update of this post. References... genome engineering...

  4. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...subpopulations of cells, you’ll soon find yourself encountering flow data in the literature. Data reported from...of flow cytometry is to use increasingly more stringent markers to identify your specific cell population...are used to separate single cells from doublets, ignoring anything not within the gate of the first plot...

  5. The Challenges of Cell Culture

    Type
    Blog Post
    ...majority of cell line problems come from people sharing cells: the black market of cell biology. Engaging...cell culture, microbiology, or virology without acquiring materials from this bank. I am often asked, “What...cells to be used and STR profiling to be conducted during projects to ensure the results are reliable. With...

  6. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    ...successful inhibition of hippocampal neurons. Initial monitoring Second, this may sound rudimentary, but before...exciting—and unanticipated—road of discovery. Monitoring behavioral output The appropriate behavioral ...science writing. Derek is also a pianist and an aspiring composer. View his blog drsimonsaysscience.org...

  7. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...The first time I heard about FRET during a journal club, my guitarist brain automatically thought about... recently and is the most rigorous method for measuring FRET. FLIM measures the fluorescence decay time...MW. Fluorescent proteins for FRET microscopy: monitoring protein interactions in living cells. Bioessays...

  8. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ...sequence in the way that they like, the design and engineering of fusion proteins is relatively straightforward...assessing oligomerization in cells. In addition to measuring stickiness, several researchers have also collected..., Marieke, et al. "A FRET-based biosensor for measuring Gα13 activation in single cells." PloS one 13.3...

  9. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...  Communication (or lack thereof): Information sharing gets more challenging the bigger an organization... can help, and if and when you find yourself transferring this skill to another job, it will be important...conflicts is not inevitable. When you know you are bringing this asset to your job, you can become a valuable...

  10. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...Immunofluorescence (IF), is an immunoassay that brings to light the cellular world. The technique allows...poorly in a given context, let your community know! Sharing your experience, positive or negative, will help...Immunofluorescence Staining. In: Methods in Molecular Biology. Springer New York, pp 299–311. https://doi.org/10.1007/...

  11. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...need to test for this in your system if you’re measuring expression over a period of weeks (in vitro) or...to turn on, or turn off, gene expression by administering an inducing agent like doxycycline (Favre et...cells you want to deliver to, and how you’ll be measuring your experimental output. You’ll also want to ...

  12. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...Hippocampus labeled with anti-Kv2.1 (magenta), anti-AnkyrinG (green), and Hoechst nuclear stain (cyan). Panels...expect the target to be. You can test this by preparing a sample without the primary antibody, or with...Yong (Ed.), Biobanking (Vol. 1897, pp. 299–311). Springer New York. https://doi.org/10.1007/978-1-4939-8935...

  13. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...make lentiviral vectors fantastic options for delivering shRNA, CRISPR/Cas9 components, and fluorescent...lines but reach their limits when it comes to transferring DNA into primary cells or non-dividing cells...virus. Even though this system was efficient at transferring the gene of interest to the appropriate cells...

  15. Introducing...Addgene Quick Tips!

    Type
    Blog Post
    ...pipetting! We’ll release a new tip every two weeks during the school year. Our first quick tip, “Your Bench...  Want your quick tips even quicker? We’ll be sharing our favorite parts of every quick tip video on ...

  16. Addgene's 20th Anniversary Party

    Type
    Blog Post
    ...collection of memorabilia, even made an appearance during co-founder and CSO Melina Fan's words of welcome...you all so much for an amazing twenty years of sharing science!       ...

  17. Congrats to the 2019 Deck The Lab Winners

    Type
    Blog Post
    ... What a fun video of Mario, dressed as Santa, delivering gifts to the Mushroom Kingdom. Kravitz Lab 2019...snowflakes, ornaments, lights, and took a group photo wearing holiday sweaters and Santa hats. There was even...

  18. Introducing Addgene's Microbiology Pages

    Type
    Blog Post
    ...Bacterial Expression Systems. Check out our Genome Engineering Overview and our CRISPR guide to find out how...how CRISPR is being used for bacterial genome engineering. To make it easier for you to find plasmids ...

  19. Addgene’s New Way to Help You Share Science

    Type
    Blog Post
    ...plasmid-based technologies. Commentary about scientific sharing and the open-access movement. At Addgene, we believe...the conversation! Do you have a question about culturing iPS cells? Confused by which system to use for...
Showing: 641 - 660 of 1010 results