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We narrowed to 994 results for: Des

Showing: 641 - 660 of 994 results

  1. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ...some point. Usually it comes up when a researcher decides on a new project and begins by trying to reproduce...they really want to do is get the paper off their desk and move on. Nevertheless, editors can and do play...

  2. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ...best serotype for their particular study, which includes comparing the transduction patterns of different...fluorescence of the sensor. This “contrast” variant provides improved detection for tracking large populations...

  3. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ... chromosome-specific loci in the genome and the guides are relatively unstable.   Figure 1: CRISPRainbow... the gRNA tetraloop and went through subsequent design iterations to increase stability and minimize misfolding...

  4. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...if you end up concentrating your AAV above your desired titer and want to dilute it, err on the side of...less buffer than you would to achieve the exact desired concentration to avoid over-diluting, and make ...

  5. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ... determine if proteins are targeted to their destination co- or post-translationally. TRICK: Visualizing... usually translated in a specific compartment. Despite these limitations, these techniques represent powerful...

  7. Addgene Depositors Get More Citations

    Type
    Blog Post
    ... Science. For each publication, Web of Science provides basic bibliographic information and uses its vast...citations due to the journal of publication. Table 1 provides an example calculation of the weights. Table 1...

  8. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ...form concatemers in later steps. This new NEB kit includes a step that both fragments the DNA and prevents...of the workshop, NEB gave a brief presentation describing the technology. Then we split into pairs to actually...

  10. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ...speakers from top minds in the stem cell field have described the Yamanaka paper as "life changing". Dr. Yamanaka...affordable. 4. Stem cell scientists are generous Besides some of the scientists I've already mentioned, ...

  11. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...mammalian plasmid components, it may be useful to describe the means of introducing genetic material (such...the plasmid in the same way. Selection methods, described below, are typically employed when creating stable...

  13. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...familiar with the basics of controls in experimental design, you’ll need a few controls specific to flow as...controls like FMO or isotype controls, which we’ll describe a bit later. Single Colors Single color controls...

  14. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...promoter should flank this component as well. On both sides of this promoter-gene combo are two long terminal...Laboratory Press; 1997. Principles of Retroviral Vector Design. Available from: https://www.ncbi.nlm.nih.gov/books...

  15. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...through a seminal paper from Peter Schultz’s lab describing a technique that allowed for intentional incorporation...reagents accessible to your lab. The solution GCE provides almost endless possibilities for researchers, ...

  16. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...biology – they can deliver materials to specific destinations, haul a variety of cargo, and even handle a ...delivery of the actual virus to which immunity is desired. Instead, components of the virus are now delivered...

  17. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...target cells and organisms, and when that cargo is designed to make you sick…well, you feel it. But the same...host genome or temporarily express whatever it encodes. This ability to infect cells with a high efficiency...

  18. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...1x1012 vg/mouse is more standard) and reflects a desire for very high MOI on top of very high coverage....researchers have used the flexibility of AAV genome design in remarkably creative ways. The continued development...

  19. Antibodies 101: Isotypes

    Type
    Blog Post
    ...molecules are recruited by the antibody to help destroy and remove a pathogen. Different isotypes also ...well as the genes that encode those proteins are designated by the lower-case Greek letters μ, δ, γ, ε, and...
Showing: 641 - 660 of 994 results