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We narrowed to 772 results for: Dos

Showing: 641 - 660 of 772 results

  3. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...replication-competent lentivirus (RCL) in your target cells.  Q4: How do the packaging systems differ between 2nd- and 3rd-generation...

  4. CRISPR Guide

    Type
    Guide
    ...to G (or T to C) change. Adenosine DNA deaminases do not exist in nature, but have been created by directed...have been developed for inhibition using dCas9. How Do You Use a CRISPR Library? CRISPR libraries from Addgene...alterations, such as small mutations or inversions, than do large deletions generated by Cas9 systems. Cas3 must..., Cogan, J. Z., Replogle, J. M., Adriaens, C., Ramadoss, G. N., Shi, Q., Hung, K. L., Samelson, A. J.,...

  5. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ...transformation . Tips and FAQ Do controls When doing ligations you should ALWAYS do a vector alone + ligase ...you know has only been thawed once before. Always do controls. See Tips and FAQ below for details. Try...situations where the 3:1 ratio is not working or when doing more complicated cloning. While 3:1 will get you...

  6. Optogenetics Guide

    Type
    Guide
    ...excitation or optogenetic inhibition. First things first: do you want to turn ON or turn OFF neurons in your experiment...Examples: iChloC, SwiChRca, Phobos, Aurora Browse Channelrhodospin plasmids . Halorhodopsins Halorhodopsins are...

  7. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ... become valuable tools for your research! If they do (or if they don't!), please consider sharing your...

  8. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ..., Freund E, Ricci MS, Carpenter JF, Lengsfeld CS. Do not drop: mechanical shock in vials causes cavitation....27776. Epub 2021 May 4. PMID: 33811659. Pace CN, Vajdos F, Fee L, Grimsley G, Gray T. How to measure and...

  9. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...ddPCR for AAV quantitation blog post.  We generally do not recommend using ELISA-based titering assays, ...determination of the median tissue culture infective dose (TCID50) (Zen et al., 2004).  To choose the optimal...

  11. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...was actually engineered to target the CNS. How well do these AAVs transduce the CNS and PNS? Both plasmids...from. Additionally, AAV.PHP.eB was used at a lower dose than it’s parent AAV.PHP.B: 1x1011 vs 1x1012 viral...blue (RGB) fluorescent protein (4a-c). When high doses of this viral cocktail are injected, by chance lots...the two green neurons near the arrows. When lower doses of virus are used in order to decrease the number...AAV.PHP.eB capsid. Both viruses were IV injected at a dose of 1x1011 vg per mouse and the level of transduction...AAV.PHP.S capsid. Both viruses were IV injected at a dose of 1x1012 vg per mouse and the level of transduction...-off transactivator (tTA), is injected at a high dose to maximize the number of cells that are labeled...

  15. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...systemic delivery of high doses of vectors are needed. Additionally, AAVs do not integrate in the host...

  17. Bioinformatics at Addgene

    Type
    Blog Post
    ...high-quality data for our DNA-based reagents. Not only do we extract as much information as possible from depositing...

  18. DNA Purification Without a Kit

    Type
    Blog Post
    ... spin columns you need to complete your research. Do you know of other nucleic acid purification methods...
Showing: 641 - 660 of 772 results