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Mobile-CRISPRi is a set of modular CRISPRi tools that can be used in diverse bacteria from various environments. It has the advantage of stable genomic integration and is easily transferred via conjugation.

Learn how rabies virus can be used to trace neuronal connections. AAV available at Addgene can help you with the process!

Learn about xCas9, an engineering Cas9 variant with broad PAM flexibility and high on-target specificity.

SapTrap improves CRISPR genomic tagging in C. elegans by offering scientists an easy one tube assembly reaction to generate high-throughput targeting vectors.

Get tips on how to monitor your protein of interest inside cells - co-localization, complex formation, and more.

Guest blogger Pooran Dewari presents results of an NgAgo survey he gave to some of the first NgAgo users. Find out how NgAgo compares to Cas9 so far.

Not getting enough DNA from your plasmid preps? This post outlines variables like endA and low copy number that could affect DNA yields and suggests steps to super-charge your plasmid preps.

Green fluorescent protein (GFP) and its derivatives can be used experimentally as fusion tags, biosensors, reporters, and more.

Addgene answers questions about lentiviral plasmids, including 2nd vs 3rd generation transfer vectors, packaging plasmids, biosafety concerns, and more.

Protocol - How to Ligate Plasmid DNA

Check out the latest Hot Plasmids, viral vectors, and antibodies in the Addgene repository!

Addgene's antibody lab member Kate describes each step in recombinant antibody production process.

Ready to produce rAAV in your own lab? This 101 post can help!

PRIDICT 2.0 is an updated software that can help you design gRNAs for PRIME editing. Read more in our guest post!

Data-driven design of Golden Gate Assembly overhangs greatly increases the number of fragments that can be used in cloning.

Pooled libraries are a powerful tool, but they can difficult to prepare! Learn the tips and tricks for taking your pooled libraries from plasmids to screening.

A recent study shows ancestral bias in CRISPR guide libraries, leading to skewed cancer dependency results, particularly in cell lines with African ancestry.

Addgene uses bioinformations to streamline our sequencing and QC workflow. Learn how we built the process in this blog.

Find three ways to purify genomic DNA, plasmid DNA, or PCR products without a commercial kit. Also, find a method for regenerating silica columns for reuse.

Transient plant leaf transformation can save you time... months in fact. Learn more about this method and get some tips and tricks for designing your transient plant leaf transformation experiment.