We narrowed to 750 results for: Dos

...microbes to visualize host/pathogen interactions.   Do you have a favorite way to use GFP?  Comment and ...

...replication-competent lentivirus (RCL) in your target cells.  Q4: How do the packaging systems differ between 2nd- and 3rd-generation...

... 2025. Catalog items are updated more frequently. Do you have suggestions for other plasmids that should...

... 2025. Catalog items are updated more frequently. Do you have suggestions for other plasmids that should...

...how to design primers for cloning purposes. What do I do if my PCR isn't working? Try adding 1µl of 25mM...36.8 μL Sterile dH 2 O (variable) Note: If you are doing multiple PCRs, save time by creating a “master mix...added to the master mix. Forward and Reverse Primers DO NOT get added to a master mix. Place reaction tubes...reverse primers to the thinned walled PCR tubes. Note: Do this before adding the master mix so you know that...according to the length of the target sequence. FAQ How do I design primers? See our reference page on how to...The ideal setup for this troubleshooting step is to do one reaction with each, and one reaction with both...templates. What does each ingredient specifically do? Template DNA: Contains the portion of DNA that we...

... become valuable tools for your research! If they do (or if they don't!), please consider sharing your...

..., Freund E, Ricci MS, Carpenter JF, Lengsfeld CS. Do not drop: mechanical shock in vials causes cavitation...

...ddPCR for AAV quantitation blog post.  We generally do not recommend using ELISA-based titering assays, ...

...evaluate tens of thousands of designs in parallel. To do this, we performed high-throughput ‘self-targeting...

... GetSet Need a set of overhangs, stat? GetSet can do that for you, complete with predicted fidelity. You...

... pooled libraries are typically DNA preps, but we do distribute several of our most popular CRISPR pooled...

...high-quality data for our DNA-based reagents. Not only do we extract as much information as possible from depositing...

... spin columns you need to complete your research. Do you know of other nucleic acid purification methods...

...in the biosynthetic pathway. Why plant scientists do transient leaf transformations The development of...

...Hoshino and Fujii, 2009). Other forms of enChIP, that do not use dCas9, require Zinc-finger or TAL proteins...

...your research, be sure to consider how its features do and don’t support reproducibility as not all ELNs...

...Agrobacterium strains see Weigel and Glazebrook, 2002. Tips: Do a colony PCR on your agrobacterium: Getting a transformant...

...single molecules - something researchers often want to do at neuronal synapses for instance. Also these tags...

...high doses of vectors are needed. Additionally, AAVs do not integrate in the host genome but still lead to...

...your sample names and guide sequences, and ICE will do the rest. There are no parameters that need optimizing...