... the interactive photo graffiti wall. I had a lot of fun playing the prototype of an interactive game ...about her grandfather’s humble beginnings as an operator of a printing press. Just as her grandfather used...the newspaper, Addgene uses E. coli bacteria to generate copies of plasmids. The community of scientists...

...opportunities, protocols, publications, and more to accelerate the pace of research into COVID-19. While this...opportunities, and community response initiatives. Accelerate the translational impact of research Universities...important for creating research synergies and accelerating the translational impact of research activities...Alliance at the CCRP2. Other groups have focused on iterating on methods for coronavirus research, sharing resources...

...from the standard UAS/GAL4 system is toxic to proliferating cells. A Cre-estrogen receptor ligand binding...about new recombinases suitable for this system. Gerald Rubin’s lab sought to make complex genome modifications...specific recombination site for each enzyme, were generated to test recombination efficiency. When expressed...PubMed. Reduction of Cre recombinase toxicity in proliferating Drosophila cells by estrogen-dependent activity...

...and Dr. Paul Stoffels from J&J. The panel was moderated by a non-scientist from Bloomberg news. Given ...who shared first authorship on their seminal, cooperative paper in Science.  Interestingly, this project...finding they have been able to use CRISPR/Cas9 to generate mice with genes knocked out in the insulin signaling..., open academic exploration in this area will accelerate the launch of clinical applications. During ...

... brand new Europe office located in London and operated in collaboration with LGC Standards. And now you... VII. During my years in research, I developed several new plasmids, including: plasmids to study the ...and shared by others to develop an anti-tumour therapy using antibodies targeting dendritic cells. These...sharing those ideas with others. This love of interacting with people and talking about science makes her...

...blot and immunofluorescence. GST  Glutathione S-Transferase (GST) is a 26 kDa tag which can be isolated with...Purification of His fusion proteins from E. coli is generally more straightforward and of a higher purity than...washing. Nonetheless, this tag can be purified with several different resin types and is a fairly small tag...and other qualities of each tag class, they are generally best suited for their primary application. Ready...

...cell line collections websites and catalogues Literature searches utilizing Google Scholar alerts and ...feature to know if your lines may be especially vulnerable can be very informative. Spoiler alert: HeLa ...information on the known features and relevant literature, or to decide which line is best for your experiments...Josef Reischig.  Contributing to Cellosaurus Generated a new cell? Or finding errors in the available...

...escape reflex. These traits, coupled with short generation times and high fecundity, make zebrafish ideal...lights (Baier et al., 2009).  Zebrafish behaviors Several behavioral assays can be done on zebrafish at 5...neurons that trigger the CPG (central pattern generator) (Wyart et al., 2009). The CPG is a neural circuit...circuit involved in locomotion that generates periodic motor commands for rhythmic movements like spontaneous...

...behaviors. Scientists continue to engineer opsins to operate at increasingly faster kinetics. The opsin ChETA...body. Administering the drug once can result in several hours of neuronal excitation or inhibition. This...sustained activation period is beneficial for use in therapeutic or behavioral applications where drawn out effects... by Designer Drugs): Chemogenetic Tools with Therapeutic Utility. Annual Review of Pharmacology and Toxicology...

...of time, especially at room temperature or at 4°C. Storing DNA: temperature and longevity You should consider...molecular biology like restriction enzymes and polymerases. In the situation where you need to use plasmid...Plasmid DNA can be stable at 4°C or even room temperature for a short period, and there are indications...

...receptor ACVR1. ACVR1 triggers appropriate bone regeneration in healthy patients. However, the R206H mutation...including Activin A. A commercially developed therapeutic antibody that binds Activin A and interferes ...interferes with inappropriate activin-ACVR1 interactions prevents FOP bone growth in mouse models. As this biologic...experience is in both vaccine subunit design and therapeutic biologic design (ADCs) along with pre-clinical...

...the the University of Cambridge and within the general public. The society organises talks by prominent...graduate students, postdocs, faculty members, and the general public. Chemical Society in University College ...studying to earn a PhD. The society encourages interactions between undergraduates and PhD students so that...develop a range of talks for both students and the general public, career events, and networking meetups. ...

...included in organizational decision-making. It is imperative that employees are thanked for their work by ...but it is worth it. This requires a focus on deliverables. It does not mean a manager must allow his team...enough of a jerk to make the rest of the team miserable.   A good manager keeps these factors in mind...decisions, but that should not be the common mode of operating.  Managing scientists I should add a few special...

... concept that these clustered repeat sequences operated as a safeguard against bacteriophages did not ... bacterial genome. Cas genes, which code for polymerases, nucleases, and helicases, were also disrupted...proteins then express and process the CRISPR loci to generate RNA, which guide a Cas nuclease to the specified...learned to circumvent the CRISPR/Cas defense by generating a single point mutation in the PAM sequence, ...

...publications—the pressure to produce is the same You get to interact with scientists just like you What is different...structures are common Small integrated teams, little hierarchy Real diversity and acceptance can be an issue...requirements for hiring more common Likelier to hire on general talent and not specific skills <- Can move between...where you want to go will help you focus on the transferable skills that you will need to get there. Additional...

...study which can be accessed here at bioRxiv. AAV2 interacts with the packaging cell's cell membrane When packaging...blocked following sucrose treatment. Moreover, several reports have shown that cells treated with sucrose...    Despite observing a positive effect on our overall yield, we were unable to demonstrate that these...-associated virus manufacturing." Human gene therapy21.10 (2010): 1251-1257. PMID: 20649475. PubMed Central...

...developed in David Liu’s lab in 2019, has added considerably more precision and flexibility to the CRISPR... repair favoring the edited strand. The first iteration of prime editor simply fused the wild-type Moloney...pairs for prime editing. Since PAM sites occur on average every ~8 base pairs on either DNA strand, many ...by double strand breaks has been widely used to generate precise changes. However, the efficiency of Cas9...system PE2, which had prime editing efficiencies on average 2.3- to 5.1-fold (though up to 45-fold) higher ...

...cell survival or proliferation, an additional challenge is that only partial or ephemeral knockdown will...from the manufacturer and/or in the published literature. Note that the antibody being published doesn... used in combination with other approaches. If several cell lines express the target at different levels...contains information about the labeling pattern of several cell lines using these two antibodies. This is ...

...visualizing genomic loci in living cells. Previous iterations of genomic loci visualization tools using CRISPR...PP7, or boxB sequences inserted into the sgRNA generates blue, green, or red colors for labeling. A combination... PP7, or boxB sequences in the same sgRNA will generate additional colors. Image from Ma et al., 2016 ... tetraloop and went through subsequent design iterations to increase stability and minimize misfolding...

...behind Gram’s crystal violet stain and the ion interactions that cause the stain retention in gram positive...When the negatively charged iodine is added, it interacts with the CV+, creating a complex within the peptidoglycan...trapping the large dye complex created by the interaction of CV+ and I- in the thick multilayered membrane...can be used in medical settings to determine the general type of bacteria present in an infection. This ...