We narrowed to 671 results for: FIE

..., and 9 in two different brain regions.  Image modified from Aschauer et al., 2013, Figure 1. Used under...

...sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with...

... annealed to target DNA (genomic DNA or a PCR amplified fragment) and treated with a high fidelity thermostable...

...modification. Whole-genome sequencing of cells modified by CRISPR indicates that the consequences of off-target...

... technologies have brought a renaissance in the field of health sciences, energy, and agriculture, its...

...within the brain, researchers inject genetically modified viruses encoding microbial rhodopsins into specific...

...genome browser and Primer3 to design primers that amplified a 500-600 bp product. The primers I designed (...

...March, three climate scientists and an economist testified before the House Science Committee about climate...

... and then passing the cells through an electric field to deflect them into appropriate containers. The...

...Next, you learned about how fluorophores can be classified into different levels of brightness and their...

...using BsaI, and promoter-gRNA fragments are PCR amplified for cloning into one of three Zeamays codon-optimized...

...plasmid” may be suitable for these purposes with modified protocols. *Note that this blog post only covers...

... several generations of optimization before the field begins to converge on a set of standard tools. Additionally...

...Bravo, J. P. K., Liu, M.-S., Hibshman, G. N., Dangerfield, T. L., Jung, K., McCool, R. S., Johnson, K. ...

...specificity or reduce the duration of Cas9 activity.  The field of optogenetics is renowned for enabling precise...

... Running a standard curve Uses many wells; purified protein not always available Calculate relative...

...and must be amplified before use (Figure 8C), following the amplification protocol specified by the depositing... eliminates foreign DNA, which scientists have modified for a wide range of genome engineering techniques...nucleotide spacer that defines the genomic target to be modified. You can therefore change the genomic target of...is the most popular Cas endonuclease, with many modified versions engineered to increase specificity and...Cas enzyme, such as Cas9 or Cas12a. These are identified in their names — AcrII proteins inhibit Cas9 ...sequence. HDR efficiency is generally low (< 10% of modified alleles). Selecting a target cut site as close... another within a target locus of about 8 bp, specified by a gRNA. There are two main types of base edits...

... many to isolate one specific DNA sequence. The field of recombinant DNA technology has overcome this ... DNA backbone. These tools are essential to the field of recombinant DNA, in which many identical DNA ...stable — Plasmids are stable long-term, either as purified DNA or within bacterial cells that have been preserved... for the ability of the plasmid to be copied (amplified) by bacteria. Antibiotic Resistance Gene Allows... propagated, selected for, and their integrity verified prior to use in an experiment. Plasmid Type Description...Luciferase Plasmids Viral Plasmids These plasmids are modified viral genomes that efficiently deliver genetic...Molecular Cloning Many common plasmids used in the field of recombinant DNA technology have been optimized...

...60 serotypes have been identified within human adenoviruses, which are classified into seven species (A...Adenoviral Vectors Wild-type adenoviruses have been modified by researchers to create recombinant adenoviral...of interest is cloned into the shuttle plasmid, verified, and linearized with the restriction enzyme PmeI...plasmids. Recombinant adenoviral plasmids are then verified for size and proper restriction digest patterns...patterns of recombination have not occurred. Once verified, the recombinant plasmid is linearized with PacI...expression, rAdV vectors are used in many research fields, especially when needing transgenes to express ...., Kinzler, K. W., & Vogelstein, B. (1998). A simplified system for generating recombinant adenoviruses...

...biomedical fields. Gene Therapy One of the major applications of rAAV vectors is in the field of gene therapy...Recombinant AAV Vectors Wild-type AAVs have been modified by researchers to create recombinant adeno-associated...produced in HEK293T cells are then extracted and purified for their use as viral vectors to deliver genes...and primate serotypes of AAV have thus far been identified, and it is likely that this number will continue...Recombinant adeno-associated viral (rAAV) vector Modified form of AAV used to deliver genetic material into...