We narrowed to 672 results for: FIE

1. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

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   Blog Post

   ... that it requires a high-concentration, column-purified DNA template.   Many thanks to Channabasavaiah...

2. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

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   Blog Post

   ...researchers to share data thereby enabling you to do modified analyses on published data if you need to. Similarly...

3. Viral Vectors 101: AAV Variables That Matter

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   Blog Post

   ..., and 9 in two different brain regions.  Image modified from Aschauer et al., 2013, Figure 1. Used under...

4. Antibodies 101: The Basics of Western Blotting

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   Blog Post

   ...sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with...

5. How to Design Your gRNA for CRISPR Genome Editing

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   Blog Post

   ...modification. Whole-genome sequencing of cells modified by CRISPR indicates that the consequences of off-target...

6. When Fidelity Matters: A frank discussion about ligase fidelity

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   Blog Post

   ... annealed to target DNA (genomic DNA or a PCR amplified fragment) and treated with a high fidelity thermostable...

7. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

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   Blog Post

   ...within the brain, researchers inject genetically modified viruses encoding microbial rhodopsins into specific...

8. A History of Genome Engineering in Popular Culture

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   Blog Post

   ... technologies have brought a renaissance in the field of health sciences, energy, and agriculture, its...

9. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

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   Blog Post

   ...genome browser and Primer3 to design primers that amplified a 500-600 bp product. The primers I designed (...

10. With an Eye Towards the Future, We Look Back at the March for Science

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    Blog Post

    ...March, three climate scientists and an economist testified before the House Science Committee about climate...

11. Antibodies 101: Designing Your First Flow Panel

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    Blog Post

    ...Next, you learned about how fluorophores can be classified into different levels of brightness and their...

12. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

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    Blog Post

    ... and then passing the cells through an electric field to deflect them into appropriate containers. The...

13. Selecting Your Plasmid Purification Kit

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    Blog Post

    ...plasmid” may be suitable for these purposes with modified protocols. *Note that this blog post only covers...

14. CRISPR 101: Cytosine Transversion Editors

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    Blog Post

    ... several generations of optimization before the field begins to converge on a set of standard tools. Additionally...

15. A Needle in a Base-Stack: Cas9 Structural Biology

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    Blog Post

    ...Bravo, J. P. K., Liu, M.-S., Hibshman, G. N., Dangerfield, T. L., Jung, K., McCool, R. S., Johnson, K. ...

16. CRISPR 101: Multiplex Expression of gRNAs

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    Blog Post

    ...using BsaI, and promoter-gRNA fragments are PCR amplified for cloning into one of three Zeamays codon-optimized...

17. Optogenetics + CRISPR, Using Light to Control Genome Editing

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    Blog Post

    ...specificity or reduce the duration of Cas9 activity.  The field of optogenetics is renowned for enabling precise...

18. Technical Design of a Western Blot

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    Blog Post

    ... Running a standard curve Uses many wells; purified protein not always available Calculate relative...

19. CRISPR Guide

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    Guide

    ...and must be amplified before use (Figure 8C), following the amplification protocol specified by the depositing... eliminates foreign DNA, which scientists have modified for a wide range of genome engineering techniques...nucleotide spacer that defines the genomic target to be modified. You can therefore change the genomic target of...is the most popular Cas endonuclease, with many modified versions engineered to increase specificity and...Cas enzyme, such as Cas9 or Cas12a. These are identified in their names — AcrII proteins inhibit Cas9 ...sequence. HDR efficiency is generally low (< 10% of modified alleles). Selecting a target cut site as close... another within a target locus of about 8 bp, specified by a gRNA. There are two main types of base edits...

20. Molecular Biology Reference

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    Guide

    ...sequence. This obstacle has been overcome by the field of recombinant DNA technology, which enables the... to a plasmid. These tools are essential to the field of recombinant DNA, in which many identical DNA ...Cloning Plasmids that are used most commonly in the field of recombinant DNA technology have been optimized... for the ability of the plasmid to be copied (amplified) by bacteria, which is an important characteristic...Stable - Plasmids are stable long-term either as purified DNA or within bacterial cells that have been preserved... promoter. Viral Plasmids - These plasmids are modified viral genomes that are used to efficiently deliver...generally propagated, selected for, and the integrity verified prior to use in an experiment. E. coli strains...