Skip to main content

We narrowed to 877 results for: HA

Showing: 641 - 660 of 877 results
  1. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...eBook, How to Get a Faculty Position. This edition has substantial expansions on the chalk talks and Zoom...I also incorporated example documents. This part has generated a lot of positive feedback. Many of these...
  2. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...applicable in DSB-induced genome engineering, it has biological importance for the repair of breaks that...directed repair and genome engineering CRISPR-Cas9 has revolutionized the genome engineering world and made...
  3. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...target is known to be absent naturally or in which it has been knocked-out/knocked-down. “No primary”, pre-... reliable in the future.  We hope that this post has shed some light on immunofluorescence. The technique...
  4. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...Cytotoxicity can also be cell type dependent: if a cell has few receptors for a specific envelope protein, it...lentiviral vector to be transported retrograde and also has higher neuronal transduction efficiency and a better...
  5. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...editing systems in bacteria. Our pCas/pTargetF system has been one of the most popular genomic editing systems...Yang lab.   In conclusion, the MUCICAT system has the following advantages: Fast and site-specific...
  6. An Introduction to Adenovirus

    Type
    Blog Post
    ... virulence (Tollefson et al., 1996) – cell death has been reported when using vectors, and immune inactivation...the infection to sometimes result in cell death, has led to interest in using adenoviral vectors for vaccine...
  7. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ...high for both plasmids, so each plasmid “thinks” it has a higher copy number than it actually does, and neither...large, low copy virulence plasmids. This strategy has been successful in displacing, or “curing” the virulence...
  8. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...mycoplasma contamination. Mycoplasma contamination has been shown to arise from a variety of sources such...line for mycoplasma and discard it if the culture has become overly confluent or too old. Ensuring that...
  9. Addgene's Website Update

    Type
    Blog Post
    ...soon as the homepage opens. You’ll notice it now has two options: “Search Repository” and “Deposit Materials...
  10. CRISPR Library Amplification

    Type
    Protocol
    ... evenly with a sterile spreader until all liquid has been absorbed by the agar. This usually takes 1-2...
  11. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...is important to determine how much phosphorserine has actually been incorporated into the full-length protein...protein. Up to 80% p-Ser incorporation into E17TAG GFP has been achieved with the Rinehart system (6); however...
Showing: 641 - 660 of 877 results