We narrowed to 768 results for: LIS

...purification; the whole cloning process is so simplistic once you have your parts. That was the motivation...

...alignment tools available. Try these resources for lists of alternatives to BLAST: ExPASy - http://www.expasy.org...

...pH changes (e.g. pHluorin, pHTomato). Keep this list handy as you plan your next experiment or to hand...

... selected and ranked based on the previously established sgRNA rules and  potential on- and off-target...

... toxic to dividing cells. The other antibiotics listed below affect continuous cellular processes (e.g...

...genome engineering in bacteria, alongside more established tools such as Cas9-recombineering, CRISPRa/i,...

...embed_player" overrideable=False, type='scriptV4', hide_playlist=True, viral_sharing=False, embed_button=False...

...answered the above questions, you can create your short list of options and then pick whichever is most accessible...

...adapted from the 1979 Patterson Protocol. When published, the Patterson Protocol was shown to purify DNA...

... of parameters influencing droplet rain in the Listeria monocytogenes prfA assay-reduction of ambiguous...

...restriction enzyme digest; however colony PCR can accomplish the same thing in less time and for less money...

... the viral mRNAs and minigenome. This can be accomplished in multiple ways: transfecting with an additional...

...to yeast transformation protocols also helped establish the yeast two-hybrid (Y2H) system as a robust ...

...grant situation. So, young researchers need to publish good publications in a short period of time and...

...it isn’t a good fit for all experiments. And the list of antibiotic options doesn’t end there. Make sure...

...work — in their system. The data is curated and published with a DOI, allowing others to reference and cite...

...within neuronal networks in living animals. To accomplish such cell specific manipulations in vivo, FLEx...

...applications of CRISPR. Since this post was originally published, we've also seen the new development of Cas13b...

...consider when designing a full gene. A non-exhaustive list includes: Codon abundance relative to cognate tRNA...

...safe-targeting guides as a control will result in a smaller list of true positive hits than when using non-targeting...