We narrowed to 765 results for: Mos

1. Plasmids 101: Mammalian Vectors

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   Blog Post

   ...similarly to that in bacteria and a table of the most commonly used selection markers are listed below...

2. Cloning

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   Guide

   ...The following guide will highlight several of the most popular cloning methods used to create recombinant...method, and for many reasons, remains one of the most popular today. Restriction enzymes, which are naturally...

3. A Novice's Perspective on Neuroscience - SfN2017

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   Blog Post

   ...looking into the details of SfN17, I expected that almost all of the attendees were going to be molecular...

4. Improved Plasmid Maps Powered by SnapGene

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   Blog Post

   ...and Primers - The new maps have the identities of most features labeled directly on them making them much...

5. Addgene’s Viral Service - Why Virus? Why Now?

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   Blog Post

   ...asked ourselves, “How can we do this in a way that most helps scientists and allows us to maintain Addgene...

6. Early Career Researcher Toolbox: Social Media for Scientists

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   Blog Post

   ...are using the platform, and tips for getting the most out of the site. I especially like the pointers ...

7. New and Upcoming Viral Vectors - May 2020

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   Blog Post

   ...beginning of April. Now, we've turned some of the most requested plasmids into lentiviral preps to speed...

8. Antibodies 101: Monoclonal Antibodies

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   Blog Post

   ...is used to identify the hybridomas producing the most specific antibodies. Finally, the selected hybridoma...

9. CasPEDIA: A Functional Classification of Cas Enzymes

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   Blog Post

   ...of each entry. For example, SpyCas9a (one of the most frequently used Cas enzymes) cleaves both strands...

10. Viral Vectors 101: The AAV Retrograde Serotype

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    Blog Post

    ...relatively low retrograde transport efficiency in most serotypes. Some viruses (e.g., rabies) naturally...

11. From Lab Bench to Leadership: Transitioning into Management as a PhD

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    Blog Post

    ...One of the most pivotal decisions many PhDs face when transitioning from academia to industry is whether...

12. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

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    Blog Post

    ...Golden Gate assembly, may also be beneficial. SLIC is most often compared to Gibson assembly, a cloning method...

13. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type

    Blog Post

    ..., cells that did not receive the GFP protein are most likely to be clustered at signals below 103, while...

14. How Addgene Creates Content

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    Blog Post

    ...guides are heavily used, our graphic designer made most of the Antibody Guide figures. This requires a fair...

15. Plasmids 101: Screens vs. Selections

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    Blog Post

    ...and the loss of those factors is assessed. The two most common methods of manipulation are genetic knockout...

16. GPCRs: How Do They Work and How Do We Study Them?

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    Blog Post

    ...propensity to associate with certain subtypes, although most are able to signal through more than one subtype...

17. Developing a cold tolerant E. coli using specialized chaperones

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    Blog Post

    ...mesophilic hosts at 0°C? That won’t work out either - most mesophilic systems would either die out or grow ...

18. A Deep Dive into BioBuilder

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    Blog Post

    ...worldwide. The BioBuilder curriculum is now taught in almost every state in the U.S. and in more than 30 countries...

19. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type

    Blog Post

    ..., a programming language favored by biologists. Almost as soon as I began, I realized that I needed a ...

20. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type

    Blog Post

    ...known to have good antibodies. The results look almost identical. Addgene: What advantages does your system...