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We narrowed to 1,023 results for: NTS

Showing: 641 - 660 of 1023 results

  1. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    ... the fluorescent toolbox Modern neuroscience experiments often rely on multiplexing, where multiple fluorescent...green, yellow, and/or red sensors in multicolor experiments. With OCaMP, researchers can image multiple brain...F0 (the measure of signal change during calcium events), Greater brightness, Less signal distortion from...above. The number of action potential (AP) spiking events are indicated below the trace; asterisk indicates...

  2. Clear and Accessible Writing

    Type
    Blog Post
    ...wait!  Done? Excellent! We asked our workshop participants to do the same thing, and even though only about... equal to the buoyant force of the solution. Contaminants of lower densities are captured at the 17%, ...syringe."Feel free to share your alt text in the comments! We learned a lot from listening to others' alt... Five Steps to Writing Grant Proposals to Grad Students Writing Scientific Manuscripts Tips for Improving...

  3. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ... how can you use these amazing tools in your experiments? Let’s unpack the myriad number of emerging viral...more! Libraries allow you to test thousands of elements simultaneously in your system instead of just ... virus to which immunity is desired. Instead, components of the virus are now delivered in other forms...features make them an ideal delivery system for human patients. Multiple viral-based gene therapies have already...

  4. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...activity is crucial for successful CRISPR editing experiments, there was a real need in the scientific community... in 2017 to enrich and identify desired CRISPR mutants (Wen et al., 2018). SRIRACCHA uses the Piggybac...activity and sgRNA efficiency as well as enrich for mutants through the presence of a fluorescent reporter.... best sgRNA to ensure success in your CRISPR experiments. But how? There have been several systems to ...

  5. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...80,000+ reagents and 700+ educational resources — including blog posts, protocols, guides, and collections...Addgenies involved). The new homepage and navigational elements help users who prefer to browse and discover items...implementation, we laid the foundations for many improvements to come. Ultimately, we would like our search... will give our new search engine a try to find reagents and educational material. Let us know what works...

  6. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ... and Lentiviral preps. This new Viral Service represents Addgene’s largest new initiative since we started...self-supporting service so that Addgene is not dependent on grants. Aiming to operate under a similar model for our...in 2018, our Product Team) continues to make improvements to our website and inventory management systems... were waiting. I look forward to sharing new developments on this project and on all the interesting things...

  7. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ...to a lacZΔM15-mutant bacterial cell in trans complements the mutation allowing for a functional enzyme...when expressed, is lethal to the cell. Cloning fragments are inserted into an MCS in the center of this... susceptible to or dependent on certain media components, and are rescued by genes supplied on the transformed...Expression Systems Find Empty Backbones for Your Experiments    ...

  8. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    ...these people you meet will review your papers or grants in the future, and don’t be surprised to know that...personally Scientists in your field will know about grants and projects that might help your work Tenure can... people. At large conferences, look for smaller events or meet-ups to connect with others more easily....conference Make sure you have a business card – even students and postdocs. There are many inexpensive/free ...

  9. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...reviewer’s comments without explaining why. If it looks like you have ignored their comments, they will...plasmids (like your Addgene plasmid #), and other reagents. F1000Research, among many other journals, has...researchers have enough information to replicate your experiments, making research more reproducible and your results...

  10. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...Scientists around the world have been making major improvements to CRISPR technology since its initial applications..., heterodimerizing domain to each of the Cas9 fragments, the authors created a photoactive Cas9 tool, ...photoactivatable repressor (dCas9). The activity of all variants was reversible and repeatable. Since these initial...in turn transcribes N-Cas9. The N- and C-Cas9 fragments then dimerize to form a functional protein due...Engineering of temperature- and light-switchable Cas9 variants. Nucleic Acids Research 44: . https://doi.org/...

  11. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...microbiome to deliver a genetic payload to the recipients using the IncP⍺-family-RP4 conjugation system...they only identified seven genera in in vitro experiments, possibly because in vitro conditions did not...The vector retains the oriT for delivery into recipients by conjugation. The labs screened for the host-range...without fixation or preparation, and deliver CRISPR components for gene disruption or CRISPRi. While designed...

  12. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...and planned out your key experiments. Unsurprisingly, many of these experiments require antibodies. You...antibody may be validated for IHC, not all IHC experiments are the same. Differences in sample processing...antibody could meet your needs.    Multiplex experiments Finally, consider whether you will be using other...

  13. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...approach or indirect approach, depending on the requirements of the specific assay. For the latter three,...indicated when the protein of interest is found in low amounts or if the conjugated signaling molecule has a weak...interest is often one of the most challenging components of any immunoimaging assay. Secondary antibodies...primary antibodies. The introduction of antibody fragments, such as nanobodies, has introduced new options...

  14. Addgene: The First Twenty Years

    Type
    Blog Post
    ...really appreciate the work you do to make sharing reagents and protocols so easy!” — Nina Jain, Harvard Medical...process by aligning institutions with standardized agreements and electronic signatures to dramatically speed...nominated for it, it just reflects how useful the reagents we created are for the community. More than 100...were useful for researchers wanting high quality reagents, and we realized we could use the same principles...

  15. All in a Twist: dsRNA

    Type
    Blog Post
    ...of dsRNA Transposable elements such as long/short interspersed nuclear elements can generate dsRNA by ...bidirectional transcription or as inverted repeats. These elements are almost always epigenetically silenced, but... are important considerations when using these reagents. RNA:DNA hybrids Persistent RNA:DNA hybrids that...

  16. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...growth instructions (temperature, strain, media supplements), suggested sequencing primers, or mammalian ... Each sequencing service will have different requirements for volume and concentration, and not all providers..., you can use DNA-editing software to perform alignments of multiple sequences to visually check for homology...Tell us your favorite sequencing tricks in the comments section below.  Additional Resources on the Addgene...

  17. Working with Nuclear Receptors

    Type
    Blog Post
    ...datasets and reagents in the field. Additional information can be found in the Datasets and Reagents sections... third phase of funding, the NURSA Consortium represents a unique funding model in nuclear receptor research...and acquiring them in a timely manner. Addgene represents an excellent solution to the problem of sharing...

  18. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    ...clusters without accumulation in subcellular compartments such as nuclear speckles. Furthermore, CRY2clust... distinguish real and artifactual postsynaptic currents (PSCs) this work shows that the artifactual PSCs...TagRFP-T fluorescence intensity at different time points, the authors tracked protein activity while also... assays. ShadowG is darker than other dark YFP mutants, it reduces the risk of spectral contamination ...

  19. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ... survive in competitive environments — like by living where no one else wants to. Some organisms manage...their host organisms to thrive in competitive environments. The genes encoding these functions form the...cryptic plasmid is among the most numerous genetic elements in the human gut. Cell, 187(5), 1206-1222.e16....

  20. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    ...labeling of neurons for tracing experiments.  Neuronal tracing experiments: While synthetic promoters with...the retina, it was more likely to have borrowed elements from a natural promoter that was active in the...labeling would be useful for neuronal tracing experiments where lower levels of labeling makes it easier...
Showing: 641 - 660 of 1023 results