We narrowed to 665 results for: SIM

...Degenerate primers Using degenerate primers is similar to error-prone PCR. Degenerate primers are a group...

...extended to multiplexed probe sets that allow the simultaneous interrogation of multiple potential SNP sites...

...brightness and improved maturation time compared to similar orange fluorescent proteins. In their work, the...

...of the phase of my life that had me listening to Simon & Garfunkel’s “I Am A Rock” on repeat in my bedroom...

...mismatches. To use this tool to detect a BRAF gene edit, simply PCR-amplify the BRAF locus from the edited cells...

...system once recombinant clones are generated.  A simple way to do this is to express lambda red genes from...

...search for the perfect way to light up your science. Simon Moe is an Associate Product Marketing Manager at...

...Wood, M. G., Learish, R., Ohana, R. F., Urh, M., Simpson, D., Mendez, J., Zimmerman, K., Otto, P., Vidugiris...

...a peer-reviewed journal. To start this process, simply enter your preprint title or a general description...

... the HNH and RuvC domains, which are named for similar nuclease folds found in other proteins. The C-terminal...

...analogous Wilcoxon tests have all been shown to give similar results, so you only need to pick your favorite...

...introduce cleavage of the target site with efficiency similar to using a single chimeric gRNA. At the same time...

... every genomic target. Due to its comparative simplicity and adaptability, CRISPR rapidly became the most... change the genomic target of the Cas enzyme by simply changing the target sequence present in the gRNA...The requirements for a CRISPR knockout assay are simple. You’ll need a Cas enzyme and a gRNA specific to...AcrVA3.1, which inhibits both Cas12a and Cas3. Similarly, some Acr proteins are specific to a particular...editors are based on cytosine base editors and similarly work by first converting cytidine to uridine. ...repress gene expression in mice and human cells. The simplest dCas9-based activators and repressors consist ...imaging has numerous advantages, including the simplicity of gRNA design, programmability for different...

...a single recombination reaction, drastically simplifying the process and reducing the amount of time required...be surrounded by specific recombination sites — similar to restriction enzyme cloning. To do this, your...experimental goal. Although Gateway cloning is a simple and efficient cloning method, Gateway vectors and...Oligonucleotide Stitching Yeast-mediated cloning is very similar in principle to Gibson cloning, but instead of ...the powerful recombination abilities of yeast. By simply transforming into yeast two (or more) fragments...

... acronyms, so if you're new to viral vectors or simply need a refresher, we've included a glossary at ... — hence the use of “retro”. Retroviruses have simpler genomes, containing only the necessary packaging...embryonic kidney 293T (HEK293T) cells (Figure 3A), similar to lentiviral packaging methods . The three plasmids...Addgene’s protocol is for lentiviral vectors, but a similar method can be applied when using gamma-retroviral...integration of the intended therapeutic. While similar to lentiviral vectors, they offer the benefit of...

...genes that contribute to a phenotype. At their simplest level, pooled libraries are single preparations...negatively affect data reproducibility. Figure 1: Simplified flow chart for amplifying and using a pooled ... conduct a pooled library screen. Figure 2: A simplified outline for using lentiviral gRNA libraries in...parallel sequencing of millions of DNA fragments simultaneously in a single reaction. As discussed above, access...

...assays that have multiple proteins to detect simultaneously. Conjugation The process of attaching signaling...conjugate or looking for antibodies validated for a similar assay type) and search again. After filtering, ..., directly compare the product information for similar antibodies, looking for ones that have identical...price, shipping availability, ease of purchase, or similar factors. Secondary antibodies Secondary antibodies...

... transcribed by the RNA polymerase. Figure 1: Simplified promoter region during transcription. Created...for transcription initiation. The -35 position, simply titled the -35 element, typically consists of the...BioRender.com. Operons Although bacterial transcription is simpler than eukaryotic transcription, bacteria still have...

...muscarinic-receptor based DREADDs. Compound 21 has similar potency as CNO, while DCZ is more potent than either..., T. L., Urban, D. J., Lee, H. M., Sciaky, N., Simmons, A., Nonneman, R. J., Huang, X. P., Hufeisen, S... PMID: 26889809 Slaviero, A. N., Gorantla, N., Simkins, J., Crespo, E. L., Ikefuama, E. C., Tree, M. O...

... acronyms, so if you're new to viral vectors or simply need a refresher, we've included a glossary at ...due to their well-established methods, relative simplicity of production, and higher titers. However, the...., Kinzler, K. W., & Vogelstein, B. (1998). A simplified system for generating recombinant adenoviruses...