Addgene

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Tips for Screening with Yeast Two Hybrid Systems

Type

Blog Post

...yeast! Many false negatives stem from or are exacerbated by expression of target proteins in heterologous...
Cpf1: A New Tool for CRISPR Genome Editing

Type

Blog Post

...Cas9’s, but with the direct repeat preceding the spacer rather than following it. The Cpf1 crRNA is also...
A Career in Grants: How To Become a Grant Professional

Type

Blog Post

...different issues, and our program didn’t stay in one place within the city infrastructure. My career path changed...
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

Type

Blog Post

...'ve found that research institutes in different places have very different cultures. In Basel, I felt ...
Your Lentiviral Plasmid FAQs Answered

Type

Blog Post

...expression of not only the transgene but also that of adjacent cellular genes. Q7: Which genes are deleted or...
Negotiating Work and Life: How to Find the Joy

Type

Blog Post

...“in the zone” and everything seems to fall into place. When the balance tips, we need to look to for support...
Overcoming the AAV Size Limitation for CRISPR Delivery

Type

Blog Post

... REPAIR (RNA Editing for Programmable A to I Replacement), system is also small enough to deliver with...
New Tools Enable CRISPRa for Neuroscience Applications

Type

Blog Post

...researchers inserted human CD8, a gene that encodes a surface protein for which there are good flow cytometric...
Designing Your Chalk Talk for the Academic Job Interview

Type

Blog Post

...talk?" So what exactly is a chalk talk? On the surface, a chalk talk is quite simple. It's you, a dry ...
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

Type

Blog Post

...all genes involved in the production of a cell surface glycan that’s required for ricin uptake. The ricin...
Transferable Skills Guide: External Collaborations

Type

Blog Post

...social media is a connector and virtual meeting place: You can follow interesting people on twitter and...
Plasmids 101: Shuttle Vectors

Type

Blog Post

...functional in the organism your experiment takes place in. In many cases, you won’t need to worry about...
Twenty Years of Sharing: Expanding Accessibility

Type

Blog Post

...Beijing Zhongyuan 2012 China Summit Pharmaceuticals International 2013 Japan Leehyo Bioscience...
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

Type

Blog Post

... 21243013. PubMed Central PMCID: PMC3193421. 4. Hacein-Bey-Abina, S., et al., LMO2-associated clonal T...
An Inside Look into Addgene's Dev Team

Type

Blog Post

...might be calling something a sample and in other places, we might be calling it an aliquot. This can lead...
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

Type

Blog Post

...Successful cloning occurs when the ccdB gene is replaced by the gene of interest. As a result, cells that...
Mesothelioma - Causes, Symptoms, and Treatment

Type

Blog Post

...Addgene's Cancer Collection Pages Learn How the ClonTracer Library Can be Used to Study Cancer Drug Resistance...
Antibiotic Resistance: An Old Solution but a New Problem

Type

Blog Post

...is cleaning and disinfecting frequently touched surfaces like tabletops, toys, gym equipment, etc. Virtually...
Changing Labor Laws Bring Increased Postdoc Wages

Type

Blog Post

... yet announced what they are doing, however, may face greater challenges. Current salary information that...
Lentiviral Guide

Type

Guide

...protein to interact with receptors at the host cell surface. The VSV-G envelope protein is commonly used in...

Showing: 641 - 660 of 720 results

Results per page:

Tips for Screening with Yeast Two Hybrid Systems

Cpf1: A New Tool for CRISPR Genome Editing

A Career in Grants: How To Become a Grant Professional

Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

Your Lentiviral Plasmid FAQs Answered

Negotiating Work and Life: How to Find the Joy

Overcoming the AAV Size Limitation for CRISPR Delivery

New Tools Enable CRISPRa for Neuroscience Applications

Designing Your Chalk Talk for the Academic Job Interview

Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

Transferable Skills Guide: External Collaborations

Plasmids 101: Shuttle Vectors

Twenty Years of Sharing: Expanding Accessibility

pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

An Inside Look into Addgene's Dev Team

Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

Mesothelioma - Causes, Symptoms, and Treatment

Antibiotic Resistance: An Old Solution but a New Problem

Changing Labor Laws Bring Increased Postdoc Wages

Lentiviral Guide

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