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We narrowed to 949 results for: ache

Showing: 641 - 660 of 949 results

  1. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ...specific components that make up a plasmid and why each is important. Our “Plasmids 101” series designed...replication within a plasmid by recruiting replication machinery proteins Antibiotic Resistance Gene Allows for...

  2. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    ...could provide a streamlined, one-size-fits-most approach. evoCAST is an important step toward that kind...prime editing with a site-specific recombinase to achieve insertion of long sequences. In their experiments...

  3. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ... array is transcribed and processed into crRNAs, each including one repeat and one spacer. In bacteria...protospacers. Repeats: Bacterial DNA that appears between each spacer in the CRISPR array, distinguishing the foreign...

  4. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    ...This post was co-authored by Susanna Stroik and Rachel Leeson. Here at Addgene, we like to help share ...Bairoch from the Swiss Institute of Bioinformatics reached out to us to tell us about a resource called Cellosaurus...

  5. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ...plasmids in a number of different experimental approaches. They first assessed the activation of different...Slocum ST, Che T, Gavin AC, McCorvy JD, Roth BL, Strachan RT (2020) TRUPATH, an open-source biosensor platform...

  9. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    ...step cloning, and overlap extension PCR. Although each type of cloning has its advantages, most scientists..., Meghan Monroy. Meghan Monroy is currently an Outreach Data Curation Intern at Addgene. She is also a...

  10. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    ...Wiles gave us a fascinating and fun look at her outreach work with fluorescent microbes. Check out her ...!). As we dive more into science education and outreach ourselves, we can a learn a lot from these ASM...

  11. Management for Scientists: Giving Feedback

    Type
    Blog Post
    ...stand clearly and routinely. They are clear with each employee about what they do well and where they ...reading try The Discipline of Teams by Jon R. Katzenbach and Douglas K. Smith or the book on which this...

  13. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    ... Your PI should be the kind of mentor that will teach you how to: interpret data, solve research problems...to ensure the PI will be a good role model and teacher. Do you admire her management style? Does he have...

  15. Gibson Assembly Protocol

    Type
    Protocol
    ... SSB protein to improve accuracy and efficiency. Each enzyme has a specific and unique function for the...Complementary DNA fragments can subsequently anneal to each other. Phusion High-Fidelity DNA Polymerase - incorporates...

  16. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    ...for science’s sake More opportunity to train and teach students Collaborations may be less “complicated...concentrated to business hours (mostly), but can often achieve more structured time management conducive to work...

  18. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...began in high school. “I had a great chemistry teacher in 10th grade and then fell in love with science...HMS) where he leads a lab of 25 people. He also teaches at HMS and has been involved in a variety of commercialization...

  19. Antibodies 101: Epitope Availability

    Type
    Blog Post
    ...been extracted from a cell, instead of when it is attached to a cell membrane.  Picking the Right Antibody... Rachel Leeson...

  20. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ...Chemogenetics Bryan Roth New serotype pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato AAV8 Molecular tool Massimo Scanziani...and resources!   This blog post was written by Rachel Leeson and Brian O'Neill.  More resources on the...
Showing: 641 - 660 of 949 results