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...spearheaded by Cynthia Fuhrmann at University of Massachusetts Medical School. It has been supported by a Steering...effective practices and disseminating career training approaches in a way that can be implemented widely in the...

...We perform over 100 transformations each week at Addgene, so we need to be cost and time efficient. We...for at least a year when stored at -80oC.   For each new batch of competent cells, don’t forget to do...

...to be an artist/designer. But there were a few teachers and 'guidance counselors' along the way who thought...steps and set aside time during the week to tackle each one.  Get the toughest steps out of the way early...

...points to collaboration and delegating as keys achieving success. Chapter 9: Science by the Pint - We learn...knowledge and continuity - two lead organizers on each program, one junior and one senior.  To learn more...

...Albert G. Richards, a teacher of dental radiography, had focused his x-ray machine not on teeth, but on...

...in person. Joanne Kamens, Melina Fan, and Ken Fan each spoke about what Addgene means to them. Ken mentioned...The Addgene lab often works as a very well-oiled machine and this post was no exception - the rest of the...

...Vecteezy.com Helps set concrete goals and helps you reach your goals Listens well and communicates clearly...without ever being demeaning Does not micromanage (teaches you to fish, doesn’t fish for you) Helps identify...

...expected titers, a customer support specialist will reach out to you during the request stage, before you ...  Rachel Leeson...

...they would be disregarded - everyone was very approachable.     Fig. 1: Examples of DNA sequencing...was done through the “Addgene 101” series, where each team gives a presentation on what they do and how...

...property rights and handling your constructs once they reach you. As with all of our resources, we will continually... our website; towards the bottom right corner of each page there’s a blue icon displaying the word “Help...

...variety.” In most cases, backbones are available to attach the gene of interest to either the N-or the C-terminus...backbone and making fusions, see this blog post from Joachim Goedhart: Fluorescent Proteins 101: GFP Fusion ...

...inquiries. The office is now focused on scientific outreach and community engagement. Information about contacting...Just a few weeks ago I became the newest Addgene Outreach Scientist - the first to be located in London ...

...could provide a streamlined, one-size-fits-most approach. evoCAST is an important step toward that kind...prime editing with a site-specific recombinase to achieve insertion of long sequences. In their experiments...

... array is transcribed and processed into crRNAs, each including one repeat and one spacer. In bacteria...protospacers. Repeats: Bacterial DNA that appears between each spacer in the CRISPR array, distinguishing the foreign...

...This post was co-authored by Susanna Stroik and Rachel Leeson. Here at Addgene, we like to help share ...Bairoch from the Swiss Institute of Bioinformatics reached out to us to tell us about a resource called Cellosaurus...

...specific components that make up a plasmid and why each is important. Our “Plasmids 101” series designed...replication within a plasmid by recruiting replication machinery proteins Antibiotic Resistance Gene Allows for...

...the research community. Questions? Feel free to reach out to us on Twitter, Facebook, LinkedIn, email,...Michelle, Morgan, Natashia, Nicole, Olivia, Pamela, Racheal, Ramsey, Randall, Ray, Rebecca, Renee, Shannon,...

...a higher signal-to-noise ratio with a lower photobleaching rate. Given the power of SunTag, they attempted... ratio Less chance of phototoxicity Reduced photobleaching Simplified, long-term single-molecule tracking...

... luciferases to track bacteria in real time, but each of these reporters has its drawbacks. Acoustic reporter...pre-collapse and post-collapse of arg2 and then arg1, each ARG’s distinct ultrasound fingerprint can be detected...

...making it accessible to students.” Making science approachable is often the most challenging part of the job...make sure the courses encompass what they want to teach.” Besides working with students and faculty, Caitlin...