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...in person. Joanne Kamens, Melina Fan, and Ken Fan each spoke about what Addgene means to them. Ken mentioned...The Addgene lab often works as a very well-oiled machine and this post was no exception - the rest of the...

...Vecteezy.com Helps set concrete goals and helps you reach your goals Listens well and communicates clearly...without ever being demeaning Does not micromanage (teaches you to fish, doesn’t fish for you) Helps identify...

...they would be disregarded - everyone was very approachable.     Fig. 1: Examples of DNA sequencing...was done through the “Addgene 101” series, where each team gives a presentation on what they do and how...

...expected titers, a customer support specialist will reach out to you during the request stage, before you ...  Rachel Leeson...

...property rights and handling your constructs once they reach you. As with all of our resources, we will continually... our website; towards the bottom right corner of each page there’s a blue icon displaying the word “Help...

...variety.” In most cases, backbones are available to attach the gene of interest to either the N-or the C-terminus...backbone and making fusions, see this blog post from Joachim Goedhart: Fluorescent Proteins 101: GFP Fusion ...

...inquiries. The office is now focused on scientific outreach and community engagement. Information about contacting...Just a few weeks ago I became the newest Addgene Outreach Scientist - the first to be located in London ...

... when a good friend, who was a science writer, reached out to me. She was leaving a writing position and...and defining a couple of key terms is a better approach. Also, appealing images and colors draw people...

...thermophiles, bacteria must be grown at lower temps. This approach only works for facultative thermophiles (high ... occurs? This is different than the engineered approach used to make eSpCas9 and SpCas9-HF1. By mining...

...expression of isolated bacterial proteins can be achieved using a bacterial expression system such as the... Susanna Bachle...

...the research community. Questions? Feel free to reach out to us on Twitter, Facebook, LinkedIn, email,...Michelle, Morgan, Natashia, Nicole, Olivia, Pamela, Racheal, Ramsey, Randall, Ray, Rebecca, Renee, Shannon,...

...a higher signal-to-noise ratio with a lower photobleaching rate. Given the power of SunTag, they attempted... ratio Less chance of phototoxicity Reduced photobleaching Simplified, long-term single-molecule tracking...

... luciferases to track bacteria in real time, but each of these reporters has its drawbacks. Acoustic reporter...pre-collapse and post-collapse of arg2 and then arg1, each ARG’s distinct ultrasound fingerprint can be detected...

...plasmids in a number of different experimental approaches. They first assessed the activation of different...Slocum ST, Che T, Gavin AC, McCorvy JD, Roth BL, Strachan RT (2020) TRUPATH, an open-source biosensor platform...

...focus on the packaging plasmid and take a look at each of its parts. Key elements of the AAV packaging ... domain. Following replication, Rep78/68 stays attached to the AAV genome and docks with the genome onto...

...specific components that make up a plasmid and why each is important. Our “Plasmids 101” series designed...replication within a plasmid by recruiting replication machinery proteins Antibiotic Resistance Gene Allows for...

...could provide a streamlined, one-size-fits-most approach. evoCAST is an important step toward that kind...prime editing with a site-specific recombinase to achieve insertion of long sequences. In their experiments...

...This post was co-authored by Susanna Stroik and Rachel Leeson. Here at Addgene, we like to help share ...Bairoch from the Swiss Institute of Bioinformatics reached out to us to tell us about a resource called Cellosaurus...

... array is transcribed and processed into crRNAs, each including one repeat and one spacer. In bacteria...protospacers. Repeats: Bacterial DNA that appears between each spacer in the CRISPR array, distinguishing the foreign...

...making it accessible to students.” Making science approachable is often the most challenging part of the job...make sure the courses encompass what they want to teach.” Besides working with students and faculty, Caitlin...