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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 921 results for: ire;

Showing: 641 - 660 of 921 results

  1. Antibodies 101: Isotypes

    Type
    Blog Post
    ...response, the variable region of an antibody is paired with any of the other isotype heavy chain constant...deleted.     Figure 2: Isotype switching requires DNA recombination of the antibody gene locus. ...

  2. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...new ligands for unknown GPCRs. You can find the entire PRESTO-TANGO kit at Addgene! TRUPATH: Bryan Roth...chemogenetics receptors, GPCRs were first mutated via site-directed mutagenesis to bind non natural ligands. These...

  8. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...ITRs serve as the origin of replication and are required for replication, packaging, and vector persistence...usually an issue as they are thought to be self-repaired during the AAV replication process (Chen et al...

  9. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...clarified, especially with regard to licensing models. Direct sharing of work from universities and research ...Deepak Krishnamurthy, Ethan Li, Pranav Vyas, Nibha Akireddy, Chew Chai, Manu Prakash, "Squid: Simplifying ...

  14. Summer SciComm: Preprints

    Type
    Blog Post
    ...scientists can receive recognition for their efforts, inspire future research, and increase their impact. Open...’ comments, and author responses can be shared directly alongside the preprint while the work is still...

  15. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...available for a large number of targets, and can require specific conjugates. And, of course, once you have...either re-develop or re-optimize your assay for an entirely new affinity reagent.    Fig. 1: A comparison...

  16. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...and the lab, you may not be able to carry out the entire problem solving process on your own, but you can...skillset is a part of your toolkit is important. Paired with your other skills, both technical and transferable...

  18. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...targeting efficiency of only 27.9% and homology-directed repair (HDR) taking place in 41.2% of edited cells...NHEJ rather than HDR. Figure 1: Homology-directed repair in targeted zygotes proceeds from the maternal...

  20. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...of ccdB, leading to positive identification of desired clones.   ...Becomes a powerful cloning tool... efficiently, as those that do not contain the desired insert should not grow.   Figure 2: Image...
Showing: 641 - 660 of 921 results