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We narrowed to 971 results for: lars

Showing: 641 - 660 of 971 results

  1. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ...could code the best operations software possible. Similarly, when the developers needed to know information...relationship-building and influence. You’ll need a large deal of influence and strong relationship-building...Technologies for the North America region. She is particularly interested in DNA sequencing tech, and the intersection...

  2. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ..., gRNA and pol III terminator. These relatively large cassettes (considering the gRNA itself is ~100 bases...advantageous to instead use a preexisting, conserved cellular machinery to process gRNAs. Hijacking tRNA processing...frequency of both indels at each locus, as well as large deletions. They detected fragment deletion with ...

  3. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    ...national recognition and the more people you know, the larger your profile in the community Job hunting outside...thing to consider is the size of the conference. Large conferences have many opportunities but can be overwhelming... overwhelming as far as meeting new people. At large conferences, look for smaller events or meet-ups ...

  4. The time and cost required to make a plasmid

    Type
    Blog Post
    ... repositories like Addgene provides large-scale savings. How large? Using Doench’s calculations and the...purchases can raise the cost anywhere from a few dollars to over a hundred. Of course, these were all 2018...

  5. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...bandwidth.  Jargon Learning, using, and retaining new vocabulary takes time and effort (otherwise it would be ...achieve!) Each field has its own framework: a particular way of organizing and connecting information ...experiences. If you’re hearing multiple people with similar misconceptions, it’s time to rework your strategy...

  6. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...Support Team here at Addgene, so troubleshooting molecular biology experiments was in the job description...fuzzy or ill-defined. In this article, I try to clarify what people mean when they ask about “problem solving...result of resolving the issue. Depending on the particular problem, you might even be expected to write ...

  7. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type
    Blog Post
    ...microscope showed us that, in monkey neurons, the large majority of the gold particles were in the cytoplasm...after it has been administered. Although we found a larger proportion on the membrane in mice neurons, most...the same cell, but not necessarily at the same subcellular compartment as the opsin or chemogenetic receptor...

  8. RNA Extraction Without A Kit

    Type
    Blog Post
    ...Check out our molecular biology protocols Find protocol videos on our website Read our Molecular Biology Guide...many samples you may have, access to one or more large, expensive ultracentrifuge.  Figure 1: An ...

  9. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ...packaging capacity of 4-5 kb limits the delivery of larger transgenes, such as base editors which have a gene...efficiency, the split editors transduced at levels similar to that of a single AAV expressing GFP. The lab...cyanobacteria Article contributed by Andrew Hempstead The Modular Cloning (MoClo) system allows synthetic biologists...

  10. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...worry about getting support from depositors. We regularly hear how much they see the value in depositing...) and even on 60 Minutes. We are blessed with a large community of scientists who support us by depositing... I checked we were ranked as the 21,806th most popular website in the world. I know, right? Upgrades to...

  11. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    ...or other synthetic biology circuits. What is a molecular biologist to do? Let’s take a look at how plasmids...expression from the constitutive promoter did not. Similar levels of expression between a plasmid and the ...stabilized promoter, deoxychromoviridans titers were similar regardless of where the genes were expressed. What...

  12. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    ...CRISPR guide RNAs (gRNAs) for genome editing in large-scale has led to the rapid generation of genome-...combinatorially modify the genome, screen for a particular phenotype, and quickly profile the resultant ...Sciences of the University of Hong Kong. He is particularly interested in developing and applying new technologies...

  13. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    ...., 2020). Some Acr proteins are specific to a particular Cas protein while others can inhibit CRISPR enzymes...can increase the chance of off-target editing particularly after the majority of on-target editing has ...Cas9 Toxicity in Human Hematopoietic Stem Cells. Molecular Therapy - Methods & Clinical Development 9:390...

  14. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    ...sequence ranging from a single nucleotide change to large insertions like the addition of a fluorophore or...components. However, this sometimes requires intracellular injections into the cytoplasm or pronucleus ... albino mice ranging from 11% to 100%, a level similar to other approaches.  Nanoblades can also be used...

  15. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    .... Sticky-end mediated gene transfer would be particularly helpful for targeting non-dividing cells, which... are 3.7 kb and 3.9 kb in length, respectively, larger than SaCas9 but smaller than SpCas9. These nucleases...et al. suggest that AsCpf1’s off-target rate is similar to that of high fidelity Cas9s eSpCas9 and SpCas9...

  16. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...point mutation or functional mutation in vivo: Similar to a conditional rescue experiment with a wildtype...sense (on) orientation in the presence of Cre. A similar strategy can be employed for "Cre-off" (i.e. your...for monitoring Cre-mediated recombination at the cellular level in the mouse. 2003 PubMed PMID: 12665802...

  17. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ... parallel, this approach should be considered. Similar to the p24 assay, titering via measurement of viral...serial dilutions of a viral prep. This method is particularly useful when vectors carrying fluorescent markers...In-Depth Fluoresence Titering Protocol Another popular approach utilizes the antibiotic resistance genes...

  18. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ... smaller than the vector) a 3 insert : 1 vector molar ratio will work just fine. We recommend around 100ng...proportionally. 1μL of ligase should be sufficient for larger ligation reactions. Because ligase buffer contains...vector Cut vector + Background due to vector re-circularization - most useful for phosphatase treated vector...vectors, this ratio is really meant to refer to the molarity of DNA ends available for ligation. Simply put...

  19. Gibson Assembly Protocol

    Type
    Protocol
    ... like... Cloning Methods Empty Backbone Guide Molecular Biology Protocols Summary In 2009 Dr. Daniel Gibson...effective method for the assembly of plasmids, and molecular biologists now use this method extensively. Why...be best when the DNA fragments are present in equimolar concentrations. The Gibson Assembly master mix...the important regions of your final plasmid, particularly the seams between the assembled parts. Tips ...

  20. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...see if any other fluorescent proteins display a similar effect (many do). Fusing EGFP to the self-labeling... tools, they’re optimistic that MagLOV can be similarly adaptable into future magnetogenetic tools.  Embracing...passive impact,” he says, noting that his team regularly shares troubleshooting tips and works with other...
Showing: 641 - 660 of 971 results