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We narrowed to 979 results for: lars

Showing: 641 - 660 of 979 results

  1. Hot Plasmids - May 2022

    Type
    Blog Post
    ...offset due to the nanobody-GFP interaction). The modularity of the system provides researchers better flexibility...comparable to commonly used CRISPR/Cas9 methods for large inserts. However, this system has a much lower error... a fluorescent protein coupled to a specific subcellular localization peptide. By combining three different...

  2. Antibodies 101: ChIP

    Type
    Blog Post
    ...for either tissues or cells. The process is very similar for both, though tissue samples do require an extra...thinking, well, these are mostly fairly standard molecular and cell biology techniques. What’s all the fuss...www.wannapik.com/   Even though ChIP requires a larger upfront investment than many other antibody applications...

  3. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...Fig. 1B). Tracers are typically delivered to a particular location and they either trace downstream from..., you can map what regions are signaling to a particular site while also activating or inhibiting gene...these vectors can exhibit spurious expression, particularly at the injection site (Fischer, KB. 2019). The...

  4. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...unsure what your population will look like for a particular marker, you can use the reference plots provided...isotype controls An isotype sample is prepared very similarly to a FMO sample: you make a sample from a combined...and then add in your isotype control for that particular marker. This enables you to identify any non-...

  5. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type
    Blog Post
    ...and not just the subset of those that return a particular result. Anything else that occurs during the ...are known. The author submits the research plan (similar to a preregistration, but usually with a fully ...Promotion Guidelines; preregistration to increase clarity in study design and analysis; removing publication...

  6. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ... almost all of the attendees were going to be molecular biologists. This was not at all the case. The ...neurobiology (and really in all of biology) require large data sets. Whether these data sets include videos... make the virus for you. Neuroscientists are particularly interested in using AAVs because they provide...

  7. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...used to investigate the effects of activating particular signaling pathways in specific cell types (typically...typically neurons). While all of these viruses are popular, pAAV-hSyn-DIO-hM4D(Gi)-mCherry contains a double...animals, where cre expression is restricted to a particular cell type. The human synapsin promoter further...

  8. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ..., gRNA and pol III terminator. These relatively large cassettes (considering the gRNA itself is ~100 bases...advantageous to instead use a preexisting, conserved cellular machinery to process gRNAs. Hijacking tRNA processing...frequency of both indels at each locus, as well as large deletions. They detected fragment deletion with ...

  9. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    ...national recognition and the more people you know, the larger your profile in the community Job hunting outside...thing to consider is the size of the conference. Large conferences have many opportunities but can be overwhelming... overwhelming as far as meeting new people. At large conferences, look for smaller events or meet-ups ...

  10. RNA Extraction Without A Kit

    Type
    Blog Post
    ...Check out our molecular biology protocols Find protocol videos on our website Read our Molecular Biology Guide...many samples you may have, access to one or more large, expensive ultracentrifuge.  Figure 1: An ...

  11. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...bandwidth.  Jargon Learning, using, and retaining new vocabulary takes time and effort (otherwise it would be ...achieve!) Each field has its own framework: a particular way of organizing and connecting information ...experiences. If you’re hearing multiple people with similar misconceptions, it’s time to rework your strategy...

  12. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...Support Team here at Addgene, so troubleshooting molecular biology experiments was in the job description...fuzzy or ill-defined. In this article, I try to clarify what people mean when they ask about “problem solving...result of resolving the issue. Depending on the particular problem, you might even be expected to write ...

  13. The time and cost required to make a plasmid

    Type
    Blog Post
    ... repositories like Addgene provides large-scale savings. How large? Using Doench’s calculations and the...purchases can raise the cost anywhere from a few dollars to over a hundred. Of course, these were all 2018...

  14. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type
    Blog Post
    ...microscope showed us that, in monkey neurons, the large majority of the gold particles were in the cytoplasm...after it has been administered. Although we found a larger proportion on the membrane in mice neurons, most...the same cell, but not necessarily at the same subcellular compartment as the opsin or chemogenetic receptor...

  15. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ...packaging capacity of 4-5 kb limits the delivery of larger transgenes, such as base editors which have a gene...efficiency, the split editors transduced at levels similar to that of a single AAV expressing GFP. The lab...cyanobacteria Article contributed by Andrew Hempstead The Modular Cloning (MoClo) system allows synthetic biologists...

  16. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...worry about getting support from depositors. We regularly hear how much they see the value in depositing...) and even on 60 Minutes. We are blessed with a large community of scientists who support us by depositing... I checked we were ranked as the 21,806th most popular website in the world. I know, right? Upgrades to...

  17. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    ...or other synthetic biology circuits. What is a molecular biologist to do? Let’s take a look at how plasmids...expression from the constitutive promoter did not. Similar levels of expression between a plasmid and the ...stabilized promoter, deoxychromoviridans titers were similar regardless of where the genes were expressed. What...

  18. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    ...CRISPR guide RNAs (gRNAs) for genome editing in large-scale has led to the rapid generation of genome-...combinatorially modify the genome, screen for a particular phenotype, and quickly profile the resultant ...Sciences of the University of Hong Kong. He is particularly interested in developing and applying new technologies...

  19. Gibson Assembly Protocol

    Type
    Protocol
    ... like... Cloning Methods Empty Backbone Guide Molecular Biology Protocols Summary In 2009 Dr. Daniel Gibson...effective method for the assembly of plasmids, and molecular biologists now use this method extensively. Why...be best when the DNA fragments are present in equimolar concentrations. The Gibson Assembly master mix...the important regions of your final plasmid, particularly the seams between the assembled parts. Tips ...

  20. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    ...sequence ranging from a single nucleotide change to large insertions like the addition of a fluorophore or...components. However, this sometimes requires intracellular injections into the cytoplasm or pronucleus ... albino mice ranging from 11% to 100%, a level similar to other approaches.  Nanoblades can also be used...
Showing: 641 - 660 of 979 results