We narrowed to 869 results for: lic
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TypeBlog Post... the bacteria were injected, limiting their applicability. They also did not scatter ultrasound nonlinearly...promoter pBAD and a low-copy number origin of replication (p15A) worked best under a wide range of inducer...
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Using Video to Share Your Science: We Share Science
TypeBlog Post...be part of the answer. Problems with written publications Online tools like ResearchGate, GoogleScholar...Freedom: The Expansion of Data Sharing in Research Publications Resources at Addgene.org Check out our Protocol... -
Replacing Paper: Tips for Choosing an Electronic lab Notebook
TypeBlog Post...priorities in most cases include IP protection, publications and funding. If any of these three pillars crashes...working for a large institution or company, site licenses might be available as well. In most cases this... -
Transferable Skills Guide: Time Management
TypeBlog Post... progress. For this, I always make sure I have explicit goals in mind for the endpoints of a task or project...instance, if I don’t have draft blog post ready for publication for tomorrow, I’d better upload that post from... -
Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping
TypeBlog Post...proteins A year after BioID became available, the Alice Ting Lab demonstrated that engineered ascorbate ... consists of a humanized biotin ligase from A. aeolicus, which lacks the DNA-binding domain (233 amino... -
Multiple Plasmids at a Low Price: Kits from Addgene
TypeBlog Post...polycistronic E. coli expression constructs. Some applications of plasmids from this kit include the production...variety of functions via different fusions. Some applications of PUF protein fusions reported in the literature... -
How to Negotiate a Successful Lab Start Up Budget
TypeBlog Post... most start-up P.I.’s. It doesn’t have to be complicated, or difficult to create this list. Start BEFORE...discounts, collaborations, access to internal award applications, etc. If this is the institute that can help... -
Plasmids 101: Common Lab E. coli Strains
TypeBlog Post...that carried out the labor-intensive process of replicating your new plasmid: the Escherichia coli bacteria... Most Popular Plasmids 101 Posts: Origin of Replication The Promoter Region – Let's Go! Mammalian Vectors... -
Easi-CRISPR: Generating Knock-In and Conditional Mouse Models
TypeBlog Post...previously made mouse model. To avoid disrupting splice donor/acceptor sites, Miura et al. (2018) recommend...Used with permission under a Creative Commons 4.0 license. The research groups responsible for Easi-CRISPR... -
Technologies Enabled by NanoLuc® Luciferase
TypeBlog Post...et al (2). In this post, I’ll cover two great applications of the NLuc technology. The first couples NLuc...fluorescence. The paper reports a wide variety of applications of the Enhanced Nano-Lanterns including monitoring... -
Technique: Probe Phage Genomes for Host Binding Proteins
TypeBlog Post... DNA from host cell nucleases (4). To further complicate matters, even when sequenced, phage genomes tend...Franklin L., et al. "Revisiting phage therapy: new applications for old resources." Trends in microbiology 23.4... -
Finding Your Perfect Job After University
TypeBlog Post...recession. As each advertised job received many applications, employers were requesting that only scientists...from other individuals projects, this can cause conflicts and favoritism, as well as affect morale and cause... -
CRISPR 101: Off-Target Effects
TypeBlog Post...sequencing. While this may be unnecessary for many applications, it is the only comprehensive method available...you’ve tried everything listed here. For some applications, this is ok, but it still must be controlled... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog Post...but, in numerous studies, optogenetics is used in slice physiology in order to directly show that, for example...22430017. 2. Fenno L, et al. The development and application of optogenetics. Annual review of neuroscience... -
CRISPR 101: Multiplex Expression of gRNAs
TypeBlog Post...and gRNAs can be inserted using Gibson Assembly or SLIC cloning methods. A BsaI-based E. coli multiplexing... gRNAs can be assembled using Gibson Assembly or SLIC cloning. gRNAs are expressed from two Drosophila... -
Technical Design of a Western Blot
TypeBlog Post...original publication and ensure that it includes validation data relevant to your application — and double-check... -
New Western Blot Protocol Video!
TypeBlog Post... Western blot and share tips for this tricky application. You can check out all of our protocols ... -
Beginner's Guide to Viral Vectors
TypeBlog Post...our Lentivirus FAQ, and our viral service pages. Click on the Image Above to Download the Guide ... -
Suggest A Plasmid You'd Like to Find at Addgene
TypeBlog Post...suggestaplasmid, the name of the plasmid and a publication where we can find the plasmid. While we cannot... -
Addgene's 20th Anniversary Party
TypeBlog Post...meeting each other's friends and families, eating delicious food, or playing one of the giant games or indoor...