We narrowed to 976 results for: nin

...demonstrated in human cells They used the Golden Gate cloning method to ligate expression cassettes of the gRNAs...CRISPR Libraries Enable Genome-Scale, Knockout Screening ...

...that it’s never too early to start networking! Learning about careers at Addgene Afterwards, the students...career paths, Michelle Healy said “You are always learning and that means it is possible to switch your field...

... help save you from performing any unnecessary cloning - the list of "Associated Plasmids" from these ...associated with this "Citing Article" - such as cloning their gene of interest into a previously deposited...

...competent cells and using a little-known reagent for streamlining the transformation step. Read on to find protocols...purchasing competent cells. However, for most everyday cloning, homemade competent cells will work just fine.  ...

...not. In the lab, you could be managing a team, planning meetings, and finding the best way to organize...organize your time. At a conference, you could be honing your science communications skills and networking. ...

...They discovered a family of CRISPR-Cas systems containing cas1, cas2, cas4, and a new gene cas14. cas14... adjacent CRISPR array and intergenic regions containing the putative tracrRNA into a plasmid and expressed...

...expression, such as pLKO.1 puro and pLKO.1 – TRC cloning vector. Lentiviral Plasmids -  If you're familiar...they were added to the repository, including beta-catenin reporter M50 Super 8x TOPFlash, scramble shRNA ...

...for the 2nd year running, and you can learn more about this useful, DREADD-containing AAV here. But the...

...having trouble getting NgAgo genome editing up and running in their labs. Pooran surveyed a CRISPR google ... need to get back to emailing (hopefully non-vacationing) scientists. You can find all of our August posts...

...future, and hopefully include some of our favorite winning recipes. Hack Day lunches These days everyone ...project. These projects could be anything from cleaning out the freezers to writing new plasmid protocols...

...identified 1,049 perturbations in several genes spanning 33 cancer types. The types of mutations they identified...targeted therapeutic interventions for cancer. This screening platform is an incredible boost to the cancer ...

...labs that already have created a transgenic GFP-containing organism,” says Hanson, “using a gene encoding...microscopy enthusiasts! Addgene has empty backbones containing GFP variants used by Sattarzadeh et al., as well...

...check it out!). After months of strategizing, designing, developing, testing - and repeat - we’re proud... a make-over as well. While in the course of redesigning the homepage, and striving to tell our story ...

...large genes, smaller binary vectors with a larger cloning capacity would be very useful: enter pSIM24! To...customized due to its modular structure. Two multiple cloning sites (MCS's) flank the M24 promoter, allowing ...

...Tom Ellis, and George Church for answering our burning questions about how they manage and motivate their...Advice for Choosing a Research Project Finding and Joining Your Dream Lab Advice for Choosing a Research Project...

...showing the effectiveness of using TRUPATH as a screening technique. Lastly, the activity of both antagonists...plasmids are available as a collection, facilitating screening across the transducerome, to gain a more complete...

...serotypes that have thus far been identified: AAV1-11. Nine regions of variability in the cap gene which differ...334 . https://doi.org/10.1007/s40259-017-0234-5  Büning H, Srivastava A (2019) Capsid Modifications for...

...and annotated from more than 45 publications, beginning with the seminal research articles that demonstrated...Addgene blog Read this blog post about genome-wide screening using CRISPR Find all of our CRISPR pooled library...

...There’s a steep learning curve for Inkscape, but there are lots of resources for learning the software. ...

...whole animal models like Drosophila melanogaster, obtaining enough material for a ChIP-seq experiment can ..., the Henikoff lab published a preprint paper outlining improvements to the initial CUT&RUN protocol, ...