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TypeBlog Post...performing these experiments have a good baseline for comparison. Performing WISH using these plasmids can result...
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Pathways Over Time Plasmids Engage Students in Functional Genomics Research
TypeBlog Post...D.A., Shoemaker, D.D., Astromoff, A., Liang, H., Anderson, K., Andre, B., Bnangham, R., Benito, R., Boeke... -
Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools
TypeBlog Post...properties and can be used in quenching, FRET (Förster resonance energy transfer), and FLIM (fluorescence lifetime... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog Post...behavior. A fact to consider: Rodents can hear ultrasonic frequencies (> 20 kHz, produced from fluorescent... -
CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9
TypeBlog Post...difficult to functionally manipulate and study in comparison to protein-coding genes (Rinn and Chang 2012)... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog Post... in the lab but also causes outbreaks of food poisoning, the difference is virulence plasmids! This category... -
Plasmids 101: Modular Cloning
TypeBlog Post...Though rarer, you can find MoClo that relies on Gibson and other non-Golden Gate cloning methods. MoClo... -
CRISPR in the Clinic
TypeBlog Post...disease and cultured cells. For example, in 2013, Jinsong Li and colleagues chose to explore CRISPR’s therapeutic... -
Viral Vectors 101: Calcium Sensors
TypeBlog Post...susceptible to artifacts and pH interference, and comparison between experiments can be more difficult due... -
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells
TypeBlog Post...Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR (2017) Programmable base editing of ... -
Early Career Researcher Toolbox: Finding Relevant Papers
TypeBlog Post...Facebook or Instagram. Key features Setting up a personalized feed is simple. You can enter as many search... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog Post... Choose from: Gateway cloning, gene synthesis, Gibson cloning, ligation-independent cloning, restriction... -
Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux
TypeBlog Post... Marnie Gelbart, PhD - Director of Programs at Personal Genetics Education Project Lisa Coleman, PhD -... -
5 Tips for Troubleshooting Viral Transductions
TypeBlog Post...experience of witnessing…nothing. For some unclear reason, cells are not getting transduced. Here, I will... -
Tips for Titering Your Lentiviral Preps
TypeBlog Post...PMID: 19300443. 2. Geraerts, Martine, et al. "Comparison of lentiviral vector titration methods."BMC biotechnology... -
Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015
TypeBlog Post... CRISPR/cas9 and more are being reported. Zhou Songyang from Baylor College of Medicine and Sun Yat-sen... -
The Materials Science of Optogenetics Experiments
TypeBlog Post...course of an experiment. There are a number of reasons for this but the primary one is that some behavioral... -
Experimenting in an Open Source lab: from CRISPR to Cats
TypeBlog Post... offers members and the public cheap access to personal manufacturing tools – a 3D printer, a laser cutter... -
Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR
TypeBlog Post...Li M, Zhou F, Li K, Cao H, Ni M, Liu Y, Gu Z, Dickerson KE, Xie S, Hon GC, Xuan Z, Zhang MQ, Shao Z, Xu... -
Polymerase Chain Reaction Overview and Applications
TypeBlog Post...droplets is determined and then analyzed using Poisson statistics to determine the concentration of the...