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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,045 results for: ins;

Showing: 331 - 340 of 1045 results

  1. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    ...blog Read our fluorescent proteins blog posts Download the fluorescent proteins 101 eBook Read our plasmids...cascade of caspases, protease enzymes that cleave proteins. Executioner caspases are activated last in the...Zhang et al., 2019). How does FlipGFP work? GFP contains 11 β-strands and one central ɑ-helix that can ...10 (β10), and (3) β-strand 11 (β11). Each part contains components and residues that are necessary for...specific protease that is commonly used to cleave proteins. This protease reporter based on sfCherry however...in FlipGFP can be applied to other fluorescent proteins to create an array of multicolor reporters that...fluorescent protein fusions Browse the fluorescent proteins plasmid kits ...

  2. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

    Type
    Blog Post
    ...structural component of atherogenic lipoproteins, shuttles lipoproteins through the circulatory systems, ...LipoGlo: A reporter for measuring ApoB bound lipoproteins Article contributed by Leah Schwiesow A leading...cardiovascular disease is driven by atherogenic lipoproteins that accumulate within the vascular wall. ApoB...formation of atherosclerotic plaques. ApoB bound lipoproteins (ApoB-LPs) are indirectly measured through their... Steven Farber and colleagues at the Carnegie Institute for Science have developed the LipoGlo system ...created an endogenous ApoB-NanoLuc reporter by inserting the NanoLuc luciferase downstream of the apoB ...requires binding of bacterial enhancer binding proteins (bEBPs) at a sequence upstream of the promoter...

  3. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ...founder, Eric Perkins, is Addgene’s Director of Science Product Management and explains the origins of ABC: ... favorite books - both scientific and not - to inspire you to read a little more this October. Science..., examining relationships between microbes and insects, mammals, and plants, among others. I particularly...lost for an age) fight in the Napoleonic Wars and against something even more sinister. 1000 pages long. ...and with the events of the times."     - Aliyah Weinstein, Marketing and Communications Manager Addgene's... Aliyah Weinstein...

  4. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...pMinDisplay vector. GPI contains a C-terminal glycosylphostidylinositol anchor. SGZ contains a PDGFR transmembrane...update our iGluSnFR post! Here, we look at the origins of the system and explore advances that have happened...happened since our original 2018 blog post. The origins of iGluSnFR   iGluSnFR was developed in 2013 by...an increase in fluorescence intensity from the inserted GFP. After characterizing iGluSnFR in vitro, showing...localization through different C-terminal anchoring domains The original iGluSnFR in the pMinDisplay backbone...introduce iGluSnFR3 in two additional anchoring domains – GPI and SGZ. Fig: 4 Sequence schematics...Fluorescent Protein Biosensors Browse All Fluorescent Proteins Find AAV Tools References Marvin, Jonathan S....

  5. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...transposon to stably integrate a reporter gene that contains a puromycin resistance gene followed by the target...the desired genome edit:  A donor plasmid that contains a puromycin resistance gene linked to an in-frame... when transfecting your cells.  A plasmid that contains Cas9. The Madison lab also created an inducible... labor-intensive, require multiple fluorescent proteins, and aren’t that efficient. To overcome these ...named GEmCherry1, due to the “genomic fragment inserted into mCherry”. However this one mutated methionine...GEmCherry2 work? The premise is that the genomic region inserted into the N-terminus of mCherry can be targeted...repaired by NHEJ which depending on the nucleotides inserted or deleted, can result in an in-frame shift of...

  6. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type
    Blog Post
    ...053 lentiviral expression vector, which contains an sgRNA insert site and a reporter for tracing the engineered...fragment with one part of an established pair of proteins (iLID and SspB) that undergo light-induced dimerization...photosynthetic organisms use for buoyancy. These proteins allow dissolved gases to diffuse in and out but... lab discovered in 2014 that these gas vesicle proteins scatter sound waves and create ultrasound contrast...reporter plasmids, each containing two fluorescent proteins and four repeats of a miRNA target site. One fluorescent...CRISPR-Cas9 system CHIME Article contributed by Aliyah Weinstein  Listen to the CRISPR podcast segment Understanding...gene editing tool on the immune system, the group inserted sgRNAs targeting various innate and adaptive immune...

  7. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...While institutions that focus on creative problem solving like the Howard Hughes Medical Institute have...technology. Monoculture and chemical spraying, mainstays of modern agriculture, are now synonymous with...control over their interaction with it, we hope to inspire and open a new conversation about GMOs.  Challenge...involves two basic components: the pigments (anthocyanins), and the plant circadian clock. Petunias have...transformation technologies like those pioneered by Ewen Mullins of Ireland’s Teagasc.  Plant biotechnology is full...business enterprises, he can be found climbing mountains or playing with his two cats. Keira Havens grew...Biophysics and Biochemistry from the Illinois Institute of Technology in 2004, she accepted a commission...

  8. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...digest to cut out the inserts followed by a five-piece ligation with the four inserts and the master plasmid...mammalian or other cells. TALENs are engineered proteins that comprise a DNA binding domain and an endonuclease...technology allows you to create a pair of designer proteins that will introduce a double-strand break in the...FokI endonuclease domain as well as fluorescent proteins used to sort the cells after the plasmids have...question – cardiomyocytes, adipocytes or neurons, for instance – to see how those mutations influence the disease...particular disease state. You could use the kit to insert all of those variants into stem cells with the ...introducing a mutation somewhere else in the genome. It remains to be seen which system is cleaner in terms of ...

  9. RNA Extraction Without A Kit

    Type
    Blog Post
    ...enzymes that degrade RNA, are especially hardy proteins that are found in and on everything, including...solution and spun down the homogenate to remove the insoluble material. Then, the homogenate was loaded onto... hours to separate the intact RNA from DNA and proteins. Although very effective at isolating total RNA...RNA could be effectively separated from DNA and proteins by a simple extraction protocol with guanidinium...in a guanidinium thiocyanate solution. However, instead of RNA separation using cesium-chloride gradients...in the top, aqueous layer, while DNA and other proteins are retained in the interphase and bottom, organic...into one step. After homogenization in TRIzol®, insoluble material is removed via centrifugation and the...

  10. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...suite of plasmids (pGT) with various replicative origins that can be used for broad or narrow range of microbial...longitudinal studies of the gut microbiome, James Collins’s lab developed a paper-based method that can be...concentration within a sample. Using this system, the Collins lab was able to detect mRNA from 10 bacteria of...methods, the ability to detect mRNA transcripts of toxins and other transcripts of interest is a benefit ...with broad host-range (Meyer, 2009). The vector retains the oriT for delivery into recipients by conjugation...microbial population dynamics in the gut can provide insight into how the microbiome responds to infection, ... (DCDC), relies on the assembly of fluorescent proteins into one bright particle that segregates into ...
Showing: 331 - 340 of 1045 results