We narrowed to 772 results for: LIS
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TypeBlog Post...it isn’t a good fit for all experiments. And the list of antibiotic options doesn’t end there. Make sure...
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Plasmids 101: Plasmid Incompatibility
TypeBlog Post...2012) Bacterial plasmid partition machinery: a minimalist approach to survival. Current Opinion in Structural... -
An Inside Look into Addgene's Dev Team
TypeBlog Post...these libraries as ingredients to a recipe. It’s unrealistic to plant, grow, and harvest grains every time... -
Tips for CRISPR Gene Editing in Mice
TypeBlog Post... yourself for potential gRNA sites, or you can utilise several online tools that do it for you. One such... -
Important Considerations in Optogenetics Behavioral Experiments
TypeBlog Post...your own neuroscience sub-discipline that has published using the technique and ask if they would be willing... -
TALEN Expression Vectors
TypeCollection...Daniel Voytas Lab plasmids CRISPR plasmids This page lists plasmid vectors into which DNA fragments encoding... products are the same). All expression vectors listed below have the CMV promoter for mammalian cell ...at their amino-terminus. The expression vectors listed below can be purchased together with the Joung ... -
SARS-CoV-2 Pseudotyped Virus
TypeCollection...present in many circulating variants. The table below lists expression plasmids that are suitable for generating...measuring infectivity and neutralizing activity are listed below: pBOBI-FLuc - Lentiviral reporter vector ... for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail help... -
Penn Vector Core Partnership with Addgene
TypeCollection...partnership to provide AAV viral preps, includes listing of transfer status for viral preps....vectors for distribution via Addgene. For a full list of Addgene's viral vectors See Addgene's Current...some viral vectors have been discontinued. See the list of discontinued viral vectors . The following vectors...information. Plasmids are available for all the items listed below. Please let us know (Link opens in a new ... -
Antibodies 101: Beyond Surface Labeling
TypeBlog Post...system, as is commonly used in western blotting or ELISAs. A popular method for indirect labeling employs... -
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells
TypeBlog Post...HDR), a distinct cellular DNA repair pathway. Accomplishing this usually involves simultaneously introducing... -
Plasmids 101: Expressing shRNAs for RNA Interference
TypeBlog Post... G., Babina, M., Bertin, N., Burroughs, A. M., Carlisle, A. J., Daub, C. O., Detmar, M., Deviatiiarov,... -
Antibodies 101: Introduction to Immunofluorescence
TypeBlog Post...principles as other antibody-based assays, like an ELISA or Western Blotting, but it allows you to visualize... -
Filming Science Videos in the Age of Social Distancing
TypeBlog Post...audience? As Addgene’s Science Video Production Specialist, I’m constantly thinking about ways to improve... -
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons
TypeBlog Post....0, with the goal of overcoming the limitations listed above. First, they screened a variety of fluorescent... -
Adeno-associated virus (AAV) Plasmids
TypeCollection...Packaging Plasmids The following tables contain a list of packaging plasmids that are useful to produce...Mutations PI AAV Plasmids This table contains a general list of plasmids that encode the AAV genome (i.e., the... for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail help... -
TALEN Guide
TypeCollection...CRISPR Plasmids Zinc Finger Consortium Originally published in Addgene's December 2011 Newsletter Overview...Halle-Wittenberg and Adam Bogdanove at Iowa State University published the nucleotide recognition code of the TAL effectors... before the end of the year. Dr. Zhang ‘s lab published a paper on TAL effectors in Nature Biotechnology... -
Lentiviral Prep Service
TypeCollection... for in vivo use. Volume and minimum titers are listed on each item's material page. Actual titers are...targeting sequences. Browse our repository for a list of gRNA plasmids targeting specific genes. For more...fluorescence. Morris Control Lentivirus Browse our list of available viruses based on plasmids in our repository... -
Antibodies 101: Multiplex Immunofluorescence
TypeBlog Post... H., Dumitras, C., Gong, B., Nguyen, K., Van Der List, D., Murray, K. D., Engebrecht, J., & Trimmer, J... -
Plasmids 101: Dimers and Multimers
TypeBlog Post...forms of colicinogenic factor E-1 from Proteus mirabilis. Biochemistry. 1968 Oct;7(10):3513-20. doi: 10.1021... -
Generating Mouse Models Using CRISPR/Cas9
TypeBlog Post... genome. However, with only 3.4% of founders establishing germline transmission of the transgene, this...