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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 725 results for: ACE

Showing: 661 - 680 of 725 results

  3. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...experiments, there is hardly any flexibility in gRNA placement (e.g., mutating a specific amino acid or introducing...

  4. A Control for All Seasons

    Type
    Blog Post
    ...2018). In this control, the primary antibody is replaced with pre-immune serum from the animal the antibody...

  13. CRISPR Guide

    Type
    Guide
    ...The target is present immediately adjacent to a P rotospacer A djacent M otif (PAM) The PAM sequence (NGG...codons that make up a gene PAM P rotospacer A djacent M otif; Sequence adjacent to the target sequence that...referred to as CRISPR ( C lustered R egularly I nterspaced S hort P alindromic R epeat) technologies. Before...for Cas-binding and a user-defined ∼20-nucleotide spacer that defines the genomic target to be modified....through interactions between the gRNA scaffold and surface-exposed positively-charged grooves on Cas9. gRNA... an active, DNA-binding configuration, with the spacer region of the gRNA left free to interact with the...Cas9 will only cleave a given locus if the gRNA spacer sequence shares sufficient homology with the target...

  14. Antibody Guide

    Type
    Guide
    ...suspended in the original media or isolated and placed in a buffer. Antibody Structure Figure 1: Structure...of 4 IgA1; IgA2 IgD Coexpressed with IgM on the surface of most immature B cells; function is unknown Monomer...by a capture antibody (sandwich). Block unused surface-binding sites in the wells with a blocking protein...sandwich ELISA, a capture antibody, bound to a surface, is used to capture the protein of interest and...In IHC, tissue sections are assayed and the extracellular matrix remains intact. The signaling molecule...enzyme. In ICC, cells have most or all of their extracellular matrix removed. Again, the signaling molecule...cells. Cells, with labeled antibodies bound to surface receptors, are “flowed” through a flow cytometer...

  15. Adenovirus Guide

    Type
    Guide
    ...included between the ITRs is limited by the physical space available inside of the adenoviral capsid and directly...packaging cell line, which not only frees up more space for genetic cargo but also improves safety. In order...ITRs and the packaging signal, which is located adjacent to the left ITR, resulting in the possibility ...contain the viral gene E4, adding 2.7 kb of packaging space. However, these constructs must be transfected into...encapsidation signal located at the left arm of the genome adjacent to the left ITR that is required for viral genome...Icosahedral Having the shape of a polyhedron with 20 faces, each being an equilateral triangle. Immunogenicity...

  16. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...included between the ITRs is limited by the physical space available inside of the small AAV capsid and directly...information to a given region. This allows researchers to trace connections between neurons, map the brain, and ...Cells AAV Purification by Iodixanol Gradient Ultracentrifugation AAV Titration by ddPCR AAV Titration by qPCR... viral vectors for enhanced gene transfer . Pharmaceutical Research, 25 (3), 489–499. https://doi.org/...by stimulating elongation and stabilizing the displaced single-stranded DNA. VA in trans Adenoviral gene...Icosahedral Having the shape of a polyhedron with 20 faces, each being an equilateral triangle. Immunogenicity...

  17. Optogenetics Guide

    Type
    Guide
    ...better tissue penetrance, which may allow you to place the optic fiber outside of the brain, rendering ... activated by the light is controlled via the placement of the optic fiber. Using viral vectors for opsin... the toxin's local concentration near the cell surface, and enabling the ion channel to function. TULIPs...

  18. Molecular Biology Reference

    Type
    Guide
    ...mL 10 µg/mL *Note: Carbenicillin can be used in place of ampicillin. Preparing Antibiotics Create a stock...Adenine, Cytosine, Guanine and Uracil (U). Uracil replaces thymine in RNA molecules. Every 3 nucleotides ...

  19. Science Guides

    Type
    Guide
    ...Read More CRISPR Class 2 C lustered R egularly I nterspaced S hort P alindromic R epeat (CRISPR) systems,...
Showing: 661 - 680 of 725 results