We narrowed to 954 results for: App;
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TypeBlog Post... Antibodies: Distinguishing Characteristics, Applications, and Information Resources. ILAR Journal 46:... the Addgene blog Learn more about research applications for antibodies in Intro to Antibodies Read about...
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Antibodies 101: Flow Cytometry Controls
TypeBlog Post...ll need a few controls specific to flow as an application. These controls will allow you to distinguish...isotype control. Pro tip! Many companies will have appropriate isotype controls linked on their commercial antibody... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog Post...function relationships or, in more practical applications, allow a protein to be more effectively used...the creation of many new tools and potential applications. But this focus on development, along with the... -
Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog Post...not yet understood, one thing is clear: this application opens a lot of doors in neuroscience. The retrograde...retrograde serotype also has promising therapeutic applications; it can enhance therapeutic delivery across ... -
How Addgene Creates Content
TypeBlog Post...antibody and content team, we identified what applications should be included and what basic information...we usually make figures in BioRender or use appropriately licensed and cited figures from papers or image... -
GPCRs: How Do They Work and How Do We Study Them?
TypeBlog Post... the appropriate ligand to activate the G proteins. It is estimated that there are approximately 950 different... -
The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It
TypeBlog Post...researchers (or your own from years ago!) do you really appreciate how few details are provided in most published...important enough to be answered and the methods are appropriate to answer those questions, the article is given... -
REPLACR Mutagenesis: Replacing In Vitro Recombination Methods
TypeBlog Post...product is then digested and ligated into an appropriate vector. Gibson Assembly, GeneArt Cloning, and...assembly methods are preferred. No such restriction applies to deletions; using REPLACR-mutagenesis, Trehan... -
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers
TypeBlog Post...over or write to us to get them. Even though we’re happy to provide them, that never works on a large scale..., or words of advice for people who may want to apply this tagging system to their own work? Mendenhall... -
CRISPR 101: Non-Homologous End Joining
TypeBlog Post...Here, we’ll discuss NHEJ, and how it impacts what happens to Cas9-mediated DSBs in the genome. Non-homologous...also be engaged by variants of the canonical Cas9 approach. A pair of CRISPR guides that flank regions of... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog Post...design,” she said. “Before it was a little like tapping in the dark - a lot of trial and error.” The structural...analyzing the data. It should be straightforward to apply the new library for use in various cell types and... -
Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur
TypeBlog Post...to today, Sample6 has changed focus and names, swapped leadership, and grown to 25 members. What is...competitions. After much learning, the focus of the application was changed, but Sample6 still leverages the ... -
Plasmids 101: Yeast Vectors
TypeBlog Post...Instead, the size and A-T content of the DNA (apparently independent of a known specific sequence) dictate...non-physiological levels. Scientists have tried varied approaches to combat these issues. One method to reduce ... -
Lab Safety for Biosafety Levels One and Two
TypeProtocol...working in the lab. Do not eat, drink, chew gum, or apply makeup in the laboratory. Before you start your ...and uncluttered. Disinfect your workspace with appropriate materials. For example, you may use 70% ethanol...your lab supervisor what is available and the appropriate contact times for each agent. Make sure you have...autoclaved. Conclusion Although simple, following appropriate BSL-1 and BSL-2 protocol goes a long way. It ... -
It’s Bioelectric! An Exciting Interdisciplinary Field of Research
TypeBlog Post... for non-neural cells, ion-based communication happens at a slightly slower scale. All living cells ...After attending this meeting I had an increased appreciation for the role of ion channels and pumps in developmental... -
Troubleshooting Your Plasmid Cloning Experiment
TypeBlog Post...sequence has high GC or AT content, increase the overlapping region of the primer to 40-60 bp. It is always...these steps, you might want to reconsider your approach, and try a different cloning strategy. Once you've... -
Plasmid Tools for Microbiome Studies
TypeBlog Post...MAGIC plasmids here Paper-based synthetic biology approach to analyze gut microbiota and host biomarkers ...standards, the assay is semi-quantitative and can approximate mRNA concentration within a sample. Using this... -
Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute
TypeBlog Post...It’s at Fred Hutch.” And I hadn’t seen it. So I applied and I got it.” Science writing at Fred Hutchinson...internship similar to Susan’s NEI internship. Application information can be found here Find tips for using... -
Lighting Up Cell Signaling with Photoswitchable Kinases
TypeBlog Post...spatiotemporal resolution than pharmacological or genetic approaches, but only a few such methods exist and they only...kinases good for? There are a number of potential applications for photoswitchable kinases. Below are a few... -
Summer SciComm Series: Cognitive Load
TypeBlog Post...physicist, etc., is an immense task (it takes, ooh, approximately the length of your average PhD to achieve!) ...not communicating as clearly as you intended (it happens to us all!) and offer a variety of options to make...