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We narrowed to 971 results for: LARS

Showing: 661 - 680 of 971 results

  1. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...see if any other fluorescent proteins display a similar effect (many do). Fusing EGFP to the self-labeling... tools, they’re optimistic that MagLOV can be similarly adaptable into future magnetogenetic tools.  Embracing...passive impact,” he says, noting that his team regularly shares troubleshooting tips and works with other...

  2. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...drug to the market usually takes over a billion dollars and at least a decade of work. The number of steps... Where publication is expected, the process is similar to academia. The only difference is that your paper...could be laid off while you’re there. Of course, similarly, an academic advisor could also lose their funding...

  3. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...cultures with intact ITRs. I use a spreadsheet similar to this one to plan my AAV packaging PEI transfections... This helps avoid multiple freeze/thaws of your larger aliquots of virus. AAV titration by qPCR Overview...in vitro and in vivo use. Current protocols in molecular biology, Chapter 16, Unit 16.25. PubMed PMID: ...

  4. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ...yourself anymore. Personally I found this to be particularly challenging. As PhD students and postdocs we...there is not a clear deadline. Finally check-in regularly, make yourself available, and encourage questions...manager for advice if you’re not sure how to face a particular situation. But keep in mind that a good manager...

  5. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...figure below). The double-MMEJ strategy looks very similar to HDR, but it is accomplished using much smaller... of MMEJ. This double MMEJ strategy looks very similar to HDR, but it is completed using much shorter ...whole-plasmid integration if you’d like to integrate a larger amount of material into the genome. Open questions...

  6. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    ...Addgene, as a proxy for global activity in our particular scientific subfields.  Plasmid requests correlate...plasmid requests correspond to publications of popular papers. Figure 1: Plasmid requests by date...-examine our global data at a finer temporal granularity. For each paper, we observed that the demand ...

  7. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...beautifully compelling photos and videos, while molecular, micro, and cell biology are often more suited...microscopy) Visually compelling. Easy to convey a large number of details without overwhelming your audience...Difficult to do in-depth storytelling. Requires regular content production. Internet "trolls"   More...

  8. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...generating a frameshift mutation. The majority of cellular repair events will generate these types of mutations...hundred bps of sequence. However, if you have a larger knock-in (200 bp – 2 kb), a dsDNA donor will need...oligonucleotides. These donors traditionally have larger homology arms in the 500 bp to 1 kb range, however...

  9. An Introduction to Adenovirus

    Type
    Blog Post
    ...hexon), as well as other intracellularly-exposed proteins and downstream cellular events, all recruit strong...Sarvetnick, N., Sherman, L., & Verma, I. (1998). Cellular immune response to adenoviral vector infected ...

  10. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    ...journal articles. From paper to plasmid Authors regularly indicate that their plasmids are available from... very likely find a useful plasmid, but what’s popular? The coveted Blue Flame is more than an award for... Tags: Identify a specific marker protein Use: Similar to “Vector Type” you can filter by experimental...

  11. CRISPR Antimicrobials

    Type
    Blog Post
    ...vectors are replication-deficient. Thus, a very large number of phagemids would be needed for an in vivo...Diana P. Pires, and Timothy K. Lu. “Engineering Modular Viral Scaffolds for Targeted Bacterial Population...Methods for Bacterial Genome Engineering Treating Muscular Dystrophy with CRISPR Gene Editing Using CRISPR...

  12. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    ... being used. After learning of it's continued popularity, I decided this protocol would be a worthy first...Addgene has also cataloged a variety of useful molecular cloning protocols, including making LB agar plates...needs. At Addgene, we’ll continue to create new molecular biology educational resources, such as protocols...

  13. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...are not always positioned correctly to target a particular gene. Furthermore, a target sequence may have... desired target locus. The PAM sequence is of particular concern when trying to edit a gene using homology...cleavage events for the VQR and VRER variants is similar to wild-type SpCas9, indicating that the variants...

  14. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...known to have replicons with broad host ranges. Popular replicons from these groups include: RK2 (IncP)...vectors for gene expression in bacteria. Methods in Molecular Biology (Clifton, N.J.), 765, 327–343. https:/...endonucleases on the broad host-range plasmid RK2. Molecular & General Genetics: MGG, 152(3), 129–135. https...

  15. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...level of Gene X in cells treated with Plasmid A is similar to the expression level of Gene X in the untreated... controls are negative controls that produce a similar product, but do not target an endogenous gene in... variable and helps ensure the phenotype isn’t singular or associated with only one aliquot/preparation...

  16. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...functional screen of your liking. This method is similar to site-directed mutagenesis (SDM) PCR, but with...Random Mutagenesis by Error-Prone PCR”. Methods in Molecular Biology In Vitro Mutagenesis Protocols, pg. 103...Measuring the Activity of Protein Variants on a Large Scale Using Deep Mutational Scanning.” Nature Protocols...

  17. Tips for Getting a Faculty Position

    Type
    Blog Post
    ... matter how many times I say this, candidates regularly try to convince me that their search committee...whiskies, but I remember the days of modest postdoc salaries all too well. If there's a group to make money...search!   Erik Lee Snapp received his PhD in Molecular Microbiology and Immunology from Oregon Health...

  18. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ..., but I always seek out information about the particular audience and venue before tailoring each presentation...peer mentors to check your slides for typos and clarity in a big room.  Finally, don’t pass up opportunities...informal poll by asking for a show of hands on a particular question. I like to ask about how many people...

  19. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...efficiency differed from 48-100% between strains. In a similar experiment, CRISPR reduced the HIV-1 copy number...evidence that an HIV-1 cure may be within our reach. Similar studies have shown that CRISPR can be used to combat...Additional Resources on the Addgene Blog Treating Muscular Dystrophy with CRISPR Gene Editing Learn to Use...

  20. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ... smaller than the vector) a 3 insert : 1 vector molar ratio will work just fine. We recommend around 100ng...proportionally. 1μL of ligase should be sufficient for larger ligation reactions. Because ligase buffer contains...vector Cut vector + Background due to vector re-circularization - most useful for phosphatase treated vector...vectors, this ratio is really meant to refer to the molarity of DNA ends available for ligation. Simply put...
Showing: 661 - 680 of 971 results