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We narrowed to 978 results for: LARS

Showing: 661 - 680 of 978 results

  1. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...point mutation or functional mutation in vivo: Similar to a conditional rescue experiment with a wildtype...sense (on) orientation in the presence of Cre. A similar strategy can be employed for "Cre-off" (i.e. your...for monitoring Cre-mediated recombination at the cellular level in the mouse. 2003 PubMed PMID: 12665802...

  2. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ... parallel, this approach should be considered. Similar to the p24 assay, titering via measurement of viral...serial dilutions of a viral prep. This method is particularly useful when vectors carrying fluorescent markers...In-Depth Fluoresence Titering Protocol Another popular approach utilizes the antibiotic resistance genes...

  3. Gibson Assembly Protocol

    Type
    Protocol
    ... like... Cloning Methods Empty Backbone Guide Molecular Biology Protocols Summary In 2009 Dr. Daniel Gibson...effective method for the assembly of plasmids, and molecular biologists now use this method extensively. Why...be best when the DNA fragments are present in equimolar concentrations. The Gibson Assembly master mix...the important regions of your final plasmid, particularly the seams between the assembled parts. Tips ...

  4. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...see if any other fluorescent proteins display a similar effect (many do). Fusing EGFP to the self-labeling... tools, they’re optimistic that MagLOV can be similarly adaptable into future magnetogenetic tools.  Embracing...passive impact,” he says, noting that his team regularly shares troubleshooting tips and works with other...

  5. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...editor experimentalist, and especially shines once large-scale prime editing experiments come into play. ...Kissling, L., Marquart, K. F., Schmidheini, L., Solari, C., Balázs, Z., Krauthammer, M., & Schwank, G....DNA double-strand break repair pathway balance. Molecular Cell, 81(10), 2216-2230.e10. https://doi.org/10.1016...

  6. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...drug to the market usually takes over a billion dollars and at least a decade of work. The number of steps... Where publication is expected, the process is similar to academia. The only difference is that your paper...could be laid off while you’re there. Of course, similarly, an academic advisor could also lose their funding...

  7. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ...yourself anymore. Personally I found this to be particularly challenging. As PhD students and postdocs we...there is not a clear deadline. Finally check-in regularly, make yourself available, and encourage questions...manager for advice if you’re not sure how to face a particular situation. But keep in mind that a good manager...

  8. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...Cas9 technology has revolutionized the fields of molecular biology and bioengineering, as it has facilitated...gRNAs Importantly, the use of truncated guides has largely been demonstrated to result in decreased mismatch...technologies. To date his group has develop several popular CRISPR-based tools for altering DNA sequence or...

  9. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...cultures with intact ITRs. I use a spreadsheet similar to this one to plan my AAV packaging PEI transfections... This helps avoid multiple freeze/thaws of your larger aliquots of virus. AAV titration by qPCR Overview...in vitro and in vivo use. Current protocols in molecular biology, Chapter 16, Unit 16.25. PubMed PMID: ...

  10. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...moist and hopefully aerated with a thin layer of molecular biology-grade agarose containing physiological... advantage of this technique is the potentially large number of single clones obtained (on occasion we...Maryland Medical School in Baltimore. She is particularly interested in signaling peptide maturation in...

  11. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...figure below). The double-MMEJ strategy looks very similar to HDR, but it is accomplished using much smaller... of MMEJ. This double MMEJ strategy looks very similar to HDR, but it is completed using much shorter ...whole-plasmid integration if you’d like to integrate a larger amount of material into the genome. Open questions...

  12. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    ... being used. After learning of it's continued popularity, I decided this protocol would be a worthy first...Addgene has also cataloged a variety of useful molecular cloning protocols, including making LB agar plates...needs. At Addgene, we’ll continue to create new molecular biology educational resources, such as protocols...

  13. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ... smaller than the vector) a 3 insert : 1 vector molar ratio will work just fine. We recommend around 100ng...proportionally. 1μL of ligase should be sufficient for larger ligation reactions. Because ligase buffer contains...vector Cut vector + Background due to vector re-circularization - most useful for phosphatase treated vector...vectors, this ratio is really meant to refer to the molarity of DNA ends available for ligation. Simply put...

  14. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...beautifully compelling photos and videos, while molecular, micro, and cell biology are often more suited...microscopy) Visually compelling. Easy to convey a large number of details without overwhelming your audience...Difficult to do in-depth storytelling. Requires regular content production. Internet "trolls"   More...

  15. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type
    Blog Post
    ... Thermo Scientific NanoDrop is one of the most popular options, so most of this post is based on reading... from a NanoDrop. The principles will apply to similar instruments from other companies, like the Implen...reagents, making it more difficult to detect. A particularly strong absorber at 230 nm is guanidine thiocyanate...

  16. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    ...Addgene, as a proxy for global activity in our particular scientific subfields.  Plasmid requests correlate...plasmid requests correspond to publications of popular papers. Figure 1: Plasmid requests by date...-examine our global data at a finer temporal granularity. For each paper, we observed that the demand ...

  17. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    ...journal articles. From paper to plasmid Authors regularly indicate that their plasmids are available from... very likely find a useful plasmid, but what’s popular? The coveted Blue Flame is more than an award for... Tags: Identify a specific marker protein Use: Similar to “Vector Type” you can filter by experimental...

  18. An Introduction to Adenovirus

    Type
    Blog Post
    ...hexon), as well as other intracellularly-exposed proteins and downstream cellular events, all recruit strong...Sarvetnick, N., Sherman, L., & Verma, I. (1998). Cellular immune response to adenoviral vector infected ...

  19. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...generating a frameshift mutation. The majority of cellular repair events will generate these types of mutations...hundred bps of sequence. However, if you have a larger knock-in (200 bp – 2 kb), a dsDNA donor will need...oligonucleotides. These donors traditionally have larger homology arms in the 500 bp to 1 kb range, however...

  20. CRISPR Antimicrobials

    Type
    Blog Post
    ...vectors are replication-deficient. Thus, a very large number of phagemids would be needed for an in vivo...Diana P. Pires, and Timothy K. Lu. “Engineering Modular Viral Scaffolds for Targeted Bacterial Population...Methods for Bacterial Genome Engineering Treating Muscular Dystrophy with CRISPR Gene Editing Using CRISPR...
Showing: 661 - 680 of 978 results