We narrowed to 765 results for: Mos
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TypeBlog Post...methods Although blue-white screening is probably the most widespread way to select for plasmids containing...
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How to Make Friends and Meet People at a Scientific Conference
TypeBlog Post...personal connections What can you do to get the most from the time and money you are spending on a conference... -
3 Challenges in Plant Synthetic Biology
TypeBlog Post...hope to inspire and open a new conversation about GMOs. Challenge #2: Technical obstacles to plant synthetic... -
Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur
TypeBlog Post...and potential conflicts that waste money and time. Most importantly, “the company does not become real until... -
Dos & Don'ts When Publishing a Scientific Manuscript
TypeBlog Post...manuscript is accepted for publication and is, in most cases, blind (meaning you don’t know who the reviewers... -
Will You Be My Mentor? Finding and Asking for Mentoring Support
TypeBlog Post...I work with and people from outside my work for almost 20 years. It is good to be a bit random because... -
Plan Your Experiment
TypeGuide... to choose which site is likely to result in the most efficient on-target cleavage. The gRNA target sequence...nature of NHEJ. In HDR gene editing experiments, most mutated alleles will not contain the desired edit... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog Post...CRISPR-Cas9 to function on its target. They name their most potent Acr-LOV fusion protein inhibitor CASANOVA... -
It’s Bioelectric! An Exciting Interdisciplinary Field of Research
TypeBlog Post... Developmental Biology meeting in Boston. The atmosphere was electric! After attending this meeting I ... -
Viral Vectors 101: An Introduction to AAV
TypeBlog Post... AAV2/8 has a type 2 ITR and a type 8 serotype. Almost all vectors contain type 2 ITRs, which comes from... -
Transferable Skills Guide: Problem Solving
TypeBlog Post...with several hypotheses on the cause. To test the most likely cause, you implement an experiment designed... -
Troubleshooting Your Plasmid Cloning Experiment
TypeBlog Post...based on the cloning strategy you followed. While most of the troubleshooting regarding this step has to... -
RANbodies: Reporter Nanobody Fusions
TypeBlog Post...stripped. HRP-RANbodies are, however, compatible with most multi-labeling immunohistochemical protocols where... -
Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute
TypeBlog Post...write anything good again.’ ” For Susan, one of the most satisfying parts of the job is having completed ... -
Visualizing Protein Turnover In Situ
TypeBlog Post...proteins in situ immunocytochemistry is perhaps the most common approach. With a good and specific antibody... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog Post...Endoplasmic Reticulum (ER). If homodimerization occurs (most likely in an antiparallel configuration), typical... -
Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages
TypeBlog Post...consensus of academic stakeholders. However, while most institutions are indeed going ahead with their planned... -
Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP
TypeBlog Post... nanobodies, the project really took off. Unlike most antibodies, nanobodies, which occur naturally in... -
Plasmids 101: Cre-lox
TypeBlog Post... in the field of mouse transgenics. A few of the most common uses are listed below. Cre-dependent gene... -
Working with Nuclear Receptors
TypeBlog Post...response programs. NRs also encompass one of the most successful targets for drugs currently available...